Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro

Yin Hua Shih, Dan Jae Lin, Kuo Wei Chang, Shih Min Hsia, Shun Yao Ko, Shyh Yuan Lee, Shui Sang Hsue, Tong Hong Wang, Yi Ling Chen, Tzong Ming Shieh

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Hinokitiol displays potent antimicrobial activity. It has been used in toothpaste and oral-care gel to improve the oral lichen planus and reduce halitosis. The aim of this study was to evaluate the antimicrobial activity of 3 different dental root canal sealers with hinokitiol (sealers+H) and their physical and biological effects. AH Plus (epoxy amine resin-based, AH), Apexit Plus (calcium-hydroxide-based, AP), and Canals (zinc-oxide-eugenol-based, CA), were used in this study. The original AH and CA exhibited strong anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity, but AP did not. The setting time, working time, flowability, film thickness, and solubility of each sealer +0.2%H complied with ISO 6876:2001. CA+0.2%H exhibited high cytotoxicity, but the others sealers+0.2%H did not. Because hinokitiol combined with Zn2+ in CA creates a synergistic effect. The physical tests of AP+0.5%-1%H complied with ISO 6876:2001, improved antimicrobial activity, inhibited inflammation genes cyclooxygenase-2 (COX-2) and hypoxia-inducible factor-1α (HIF-1α) mRNA in MG-63 cells and human gingival fibroblasts (HGF), and down-regulated lysyl oxidase (LOX) mRNA of HGF. In summary, AH and CA demonstrated strong antimicrobial activity, but AP did not. Application of hinokitiol increases AH anti-MRSA activity should less than 0.2% for keep well flowability. AP+0.5%-1% hinokitiol exhibited strong physical, antibacterial, and antiinflammation potentials, and inhibited S. aureus abscess formation. Applying an appreciable proportion of hinokitiol to epoxy-amine-resin-based and calcium-hydroxide-based root canal sealers is warranted, but the enhanced cytotoxicity and synergistic effect must be considered.

Original languageEnglish
Article numbere94941
JournalPLoS One
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 10 2014

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Tooth Root
Dental Pulp Cavity
teeth
anti-infective agents
inflammation
Inflammation
calcium hydroxide
amines
epoxides
resins
Epoxy Resins
fibroblasts
Calcium Hydroxide
cytotoxicity
mouth
Methicillin
halitosis
Transcription Factor AP-1
Fibroblasts
Cytotoxicity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro. / Shih, Yin Hua; Lin, Dan Jae; Chang, Kuo Wei; Hsia, Shih Min; Ko, Shun Yao; Lee, Shyh Yuan; Hsue, Shui Sang; Wang, Tong Hong; Chen, Yi Ling; Shieh, Tzong Ming.

In: PLoS One, Vol. 9, No. 6, e94941, 10.06.2014.

Research output: Contribution to journalArticle

Shih, Yin Hua ; Lin, Dan Jae ; Chang, Kuo Wei ; Hsia, Shih Min ; Ko, Shun Yao ; Lee, Shyh Yuan ; Hsue, Shui Sang ; Wang, Tong Hong ; Chen, Yi Ling ; Shieh, Tzong Ming. / Evaluation physical characteristics and comparison antimicrobial and anti-inflammation potentials of dental root canal sealers containing hinokitiol in vitro. In: PLoS One. 2014 ; Vol. 9, No. 6.
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abstract = "Hinokitiol displays potent antimicrobial activity. It has been used in toothpaste and oral-care gel to improve the oral lichen planus and reduce halitosis. The aim of this study was to evaluate the antimicrobial activity of 3 different dental root canal sealers with hinokitiol (sealers+H) and their physical and biological effects. AH Plus (epoxy amine resin-based, AH), Apexit Plus (calcium-hydroxide-based, AP), and Canals (zinc-oxide-eugenol-based, CA), were used in this study. The original AH and CA exhibited strong anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity, but AP did not. The setting time, working time, flowability, film thickness, and solubility of each sealer +0.2{\%}H complied with ISO 6876:2001. CA+0.2{\%}H exhibited high cytotoxicity, but the others sealers+0.2{\%}H did not. Because hinokitiol combined with Zn2+ in CA creates a synergistic effect. The physical tests of AP+0.5{\%}-1{\%}H complied with ISO 6876:2001, improved antimicrobial activity, inhibited inflammation genes cyclooxygenase-2 (COX-2) and hypoxia-inducible factor-1α (HIF-1α) mRNA in MG-63 cells and human gingival fibroblasts (HGF), and down-regulated lysyl oxidase (LOX) mRNA of HGF. In summary, AH and CA demonstrated strong antimicrobial activity, but AP did not. Application of hinokitiol increases AH anti-MRSA activity should less than 0.2{\%} for keep well flowability. AP+0.5{\%}-1{\%} hinokitiol exhibited strong physical, antibacterial, and antiinflammation potentials, and inhibited S. aureus abscess formation. Applying an appreciable proportion of hinokitiol to epoxy-amine-resin-based and calcium-hydroxide-based root canal sealers is warranted, but the enhanced cytotoxicity and synergistic effect must be considered.",
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AU - Hsia, Shih Min

AU - Ko, Shun Yao

AU - Lee, Shyh Yuan

AU - Hsue, Shui Sang

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AU - Chen, Yi Ling

AU - Shieh, Tzong Ming

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