Abstract
Aim: To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. Methods: A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy: normal control group (25 patients) and gastric cancer group (29 patients). Both groups were further divided into H pylori (+) and H pylori (-) subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique (HelicoBlot 2.0, Genelabs Diagnostics, Singapore). Results: The positive rate of the immunoblotting test was as high as 88.9% in the H pylori (-) gastric cancer group and only 14.3% in the H pylori (-) normal control group with a statistically significant difference. Conclusion: The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 7029-7032 |
| Number of pages | 4 |
| Journal | World Journal of Gastroenterology |
| Volume | 12 |
| Issue number | 43 |
| Publication status | Published - Nov 21 2006 |
Fingerprint
Keywords
- Enzyme-linked immunosorbent assay
- Gastric cancer
- H pylori
- Immunoblotting
- Western blot
ASJC Scopus subject areas
- Gastroenterology
Cite this
Evaluation of the role of H pylori infection in pathogenesis of gastric cancer by immunoblot assay. / Yang, Kuo Ching; Chu, Alexander; Liao, Chao Sheng; Lin, Yu Min; Wang, Gen Min.
In: World Journal of Gastroenterology, Vol. 12, No. 43, 21.11.2006, p. 7029-7032.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of the role of H pylori infection in pathogenesis of gastric cancer by immunoblot assay
AU - Yang, Kuo Ching
AU - Chu, Alexander
AU - Liao, Chao Sheng
AU - Lin, Yu Min
AU - Wang, Gen Min
PY - 2006/11/21
Y1 - 2006/11/21
N2 - Aim: To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. Methods: A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy: normal control group (25 patients) and gastric cancer group (29 patients). Both groups were further divided into H pylori (+) and H pylori (-) subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique (HelicoBlot 2.0, Genelabs Diagnostics, Singapore). Results: The positive rate of the immunoblotting test was as high as 88.9% in the H pylori (-) gastric cancer group and only 14.3% in the H pylori (-) normal control group with a statistically significant difference. Conclusion: The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.
AB - Aim: To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. Methods: A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy: normal control group (25 patients) and gastric cancer group (29 patients). Both groups were further divided into H pylori (+) and H pylori (-) subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique (HelicoBlot 2.0, Genelabs Diagnostics, Singapore). Results: The positive rate of the immunoblotting test was as high as 88.9% in the H pylori (-) gastric cancer group and only 14.3% in the H pylori (-) normal control group with a statistically significant difference. Conclusion: The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.
KW - Enzyme-linked immunosorbent assay
KW - Gastric cancer
KW - H pylori
KW - Immunoblotting
KW - Western blot
UR - http://www.scopus.com/inward/record.url?scp=33751532920&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751532920&partnerID=8YFLogxK
M3 - Article
C2 - 17109500
AN - SCOPUS:33751532920
VL - 12
SP - 7029
EP - 7032
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
SN - 1007-9327
IS - 43
ER -