Abstract
Aim: To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. Methods: A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy: normal control group (25 patients) and gastric cancer group (29 patients). Both groups were further divided into H pylori (+) and H pylori (-) subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique (HelicoBlot 2.0, Genelabs Diagnostics, Singapore). Results: The positive rate of the immunoblotting test was as high as 88.9% in the H pylori (-) gastric cancer group and only 14.3% in the H pylori (-) normal control group with a statistically significant difference. Conclusion: The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 7029-7032 |
| Number of pages | 4 |
| Journal | World Journal of Gastroenterology |
| Volume | 12 |
| Issue number | 43 |
| Publication status | Published - Nov 21 2006 |
Keywords
- Enzyme-linked immunosorbent assay
- Gastric cancer
- H pylori
- Immunoblotting
- Western blot
ASJC Scopus subject areas
- Gastroenterology