Evaluation of the potential hypoglycemic and beta-cell protective constituents isolated from corni fructus to tackle insulin-dependent diabetes mellitus

Mei Hsiang Lin, Hui Kang Liu, Wei Jan Huang, Chu Chun Huang, Tzu Hua Wu, Feng-Lin Hsu

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44 Citations (Scopus)

Abstract

Corni fructus is the fruit of Cornus officinalis Sieb. et Zucc. and has attracted much interest due to its traditional applications and active fraction that reportedly possesses antidiabetic effects. In this study, we isolated 12 compounds from Corni fructus including three flavonoids, two iridoid glycosides, three phenolic compounds, and two triterpenoids, together with cornuside (11) and 2-butoxybutanedioic acid (12). Chemical structures were identified by 1H, 13C NMR, DEPT, COSY, HSQC, and HMBC spectral analyses. Furthermore, the glucose uptake efficiency, messenger (m)RNA expression of phosphoenolpyruvate carboxykinase (PEPCK), and prevention of cytokine-mediated cytotoxicity in the presence of test agents were evaluated. While CH and CB significantly increased glucose uptake from muscle, compounds 3 and 8, each at 50 μM, significantly suppressed PEPCK mRNA expression. Finally, compound 5, at 50 and 100 μM, effectively attenuated β-cell death. In conclusion, those compounds could contribute to the antihyperglycemic and β-cell-protective actions of Corni fructus against diabetes mellitus.

Original languageEnglish
Pages (from-to)7743-7751
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number14
DOIs
Publication statusPublished - Jul 27 2011

Fingerprint

Cornus officinalis
Cornus
Phosphoenolpyruvate
insulin-dependent diabetes mellitus
Medical problems
Type 1 Diabetes Mellitus
Hypoglycemic Agents
Iridoid Glycosides
Insulin
Glucose
Cell death
Cytotoxicity
Fruits
Flavonoids
Muscle
Nuclear magnetic resonance
cells
RNA
hypoglycemic agents
Cytokines

Keywords

  • beta cells
  • Corni fructus
  • cytokine
  • Diabetes mellitus
  • glucose uptake
  • Liuwei Dihuang Wan
  • phosphoenolpyruvate carboxykinase (PEPCK)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

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abstract = "Corni fructus is the fruit of Cornus officinalis Sieb. et Zucc. and has attracted much interest due to its traditional applications and active fraction that reportedly possesses antidiabetic effects. In this study, we isolated 12 compounds from Corni fructus including three flavonoids, two iridoid glycosides, three phenolic compounds, and two triterpenoids, together with cornuside (11) and 2-butoxybutanedioic acid (12). Chemical structures were identified by 1H, 13C NMR, DEPT, COSY, HSQC, and HMBC spectral analyses. Furthermore, the glucose uptake efficiency, messenger (m)RNA expression of phosphoenolpyruvate carboxykinase (PEPCK), and prevention of cytokine-mediated cytotoxicity in the presence of test agents were evaluated. While CH and CB significantly increased glucose uptake from muscle, compounds 3 and 8, each at 50 μM, significantly suppressed PEPCK mRNA expression. Finally, compound 5, at 50 and 100 μM, effectively attenuated β-cell death. In conclusion, those compounds could contribute to the antihyperglycemic and β-cell-protective actions of Corni fructus against diabetes mellitus.",
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AU - Liu, Hui Kang

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AU - Huang, Chu Chun

AU - Wu, Tzu Hua

AU - Hsu, Feng-Lin

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