Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma

Hsueh Wei Chang, Ching Yu Yen, Chung Ho Chen, Jun Hsu Tsai, Jen Yang Tang, Yung Ting Chang, Yu Hsun Kao, Yen Yun Wang, Shyng Shiou F. Yuan, Sheng Yang Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Integrin signaling may modulate several different functions involved in cell migration, invasion, proliferation and motility, and is a potential candidate biomarker for oral cancer. In the present study, a total of four integrin genes were evaluated as potential biomarkers of oral squamous cell carcinoma (OSCC). Gene expression was determined using the reverse transcription-quantitative polymerase chain reaction in 55 OSCC and 55 matched normal oral tissues. The performance of individual and combined biomarkers was analyzed by receiver operating characteristic (ROC) analysis based on the relative mRNA expression (OSCC vs. matched oral tissue from the tumor-free margin), which was calculated using the ΔΔCq value (ΔCq of OSCC-ΔCq of oral tissue from the tumor-free margin of the same patient). In the individual ROC analysis, the areas under the ROC curve (AUCs) of relative mRNA expression (ΔΔCq) of integrin subunit α3 (ITGA3), integrin subunit α5 (ITGA5), integrin subunit β1 (ITGB1) and integrin subunit β6 (ITGB6) in all tumor locations were 0.724, 0.698, 0.640 and 0.657, respectively. For locations 2 (tongue/mouth part) and 3 (edentulous ridge), their individual AUC values were 0.840, 0.765, 0.725 and 0.763, respectively. In the cumulative ROC analysis, ITGA3, ITGA5 and ITGB1 genes exhibited the highest combined AUC values (0.809 and 0.871 for all locations and locations 2 and 3 combined, respectively) compared with other biomarker combinations. In conclusion, the results of the present study identified that higher mRNA expressions of ITGA3, ITGA5, ITGB1 and ITGB6 genes are suitable for OSCC diagnosis biomarkers. Cumulative ROC analysis indicated an improved overall performance compared with the best individual integrin biomarker of OSCC.

Original languageEnglish
Pages (from-to)4773-4781
Number of pages9
JournalOncology Letters
Volume16
Issue number4
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

Tumor Biomarkers
Integrins
Squamous Cell Carcinoma
Messenger RNA
ROC Curve
Biomarkers
Area Under Curve
Cell Movement
Genes
Mouth Neoplasms
Tongue
Reverse Transcription
Mouth
Gene Expression
Polymerase Chain Reaction

Keywords

  • Integrin
  • Oral cancer
  • Receiver-operating characteristic curves
  • Tumor biomarker

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma. / Chang, Hsueh Wei; Yen, Ching Yu; Chen, Chung Ho; Tsai, Jun Hsu; Tang, Jen Yang; Chang, Yung Ting; Kao, Yu Hsun; Wang, Yen Yun; Yuan, Shyng Shiou F.; Lee, Sheng Yang.

In: Oncology Letters, Vol. 16, No. 4, 01.10.2018, p. 4773-4781.

Research output: Contribution to journalArticle

Chang, Hsueh Wei ; Yen, Ching Yu ; Chen, Chung Ho ; Tsai, Jun Hsu ; Tang, Jen Yang ; Chang, Yung Ting ; Kao, Yu Hsun ; Wang, Yen Yun ; Yuan, Shyng Shiou F. ; Lee, Sheng Yang. / Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma. In: Oncology Letters. 2018 ; Vol. 16, No. 4. pp. 4773-4781.
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abstract = "Integrin signaling may modulate several different functions involved in cell migration, invasion, proliferation and motility, and is a potential candidate biomarker for oral cancer. In the present study, a total of four integrin genes were evaluated as potential biomarkers of oral squamous cell carcinoma (OSCC). Gene expression was determined using the reverse transcription-quantitative polymerase chain reaction in 55 OSCC and 55 matched normal oral tissues. The performance of individual and combined biomarkers was analyzed by receiver operating characteristic (ROC) analysis based on the relative mRNA expression (OSCC vs. matched oral tissue from the tumor-free margin), which was calculated using the ΔΔCq value (ΔCq of OSCC-ΔCq of oral tissue from the tumor-free margin of the same patient). In the individual ROC analysis, the areas under the ROC curve (AUCs) of relative mRNA expression (ΔΔCq) of integrin subunit α3 (ITGA3), integrin subunit α5 (ITGA5), integrin subunit β1 (ITGB1) and integrin subunit β6 (ITGB6) in all tumor locations were 0.724, 0.698, 0.640 and 0.657, respectively. For locations 2 (tongue/mouth part) and 3 (edentulous ridge), their individual AUC values were 0.840, 0.765, 0.725 and 0.763, respectively. In the cumulative ROC analysis, ITGA3, ITGA5 and ITGB1 genes exhibited the highest combined AUC values (0.809 and 0.871 for all locations and locations 2 and 3 combined, respectively) compared with other biomarker combinations. In conclusion, the results of the present study identified that higher mRNA expressions of ITGA3, ITGA5, ITGB1 and ITGB6 genes are suitable for OSCC diagnosis biomarkers. Cumulative ROC analysis indicated an improved overall performance compared with the best individual integrin biomarker of OSCC.",
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