Evaluation of the increase in permeability of the blood-brain barrier during tumor progression after pulsed focused ultrasound

Feng Yi Yang, Hsin Ell Wang, Guan Liang Lin, Hui Hsien Lin, Tai-Tong Wong

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Purpose: The purpose of this study was to evaluate the permeability of the blood-brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio. Materials and methods: F98 glioma-bearing Fischer 344 rats were injected intravenously with Evans blue with or without blood-tumor barrier disruption induced by transcranial pulsed high-intensity focused ultrasound. Sonication was applied at a frequency of 1 MHz with a 5% duty cycle and a repetition frequency of 1 Hz. The permeability of the blood-brain barrier was assessed by the extravasation of Evans blue. Contrast-enhanced magnetic resonance images were used to monitor the gadolinium deposition path associated with transcranial pulsed high-intensity focused ultrasound, and the influencing size and location was also investigated. In addition, whole brain histological analysis was performed. The results were compared by two-tailed unpaired t-test. Results: The accumulation of Evans blue in brains and the tumor:ipsilateral brain permeability ratio of Evans blue were significantly increased after pulsed high-intensity focused ultrasound exposure. Evans blue injection followed by sonication showed an increase in the tumor:ipsilateral brain ratio of the target tumors (9.14:1) of about 2.23-fold compared with the control tumors (x4.09) on day 6 after tumor implantation. Magnetic resonance images showed that pulsed high-intensity focused ultrasound locally enhances the permeability of the blood-tumor barrier in the glioma-bearing rats. Conclusion: This method could allow enhanced synergistic effects with respect to other brain tumor treatment regimens.

Original languageEnglish
Pages (from-to)723-730
Number of pages8
JournalInternational Journal of Nanomedicine
Volume7
DOIs
Publication statusPublished - Dec 5 2012
Externally publishedYes

Keywords

  • Blood-brain barrier
  • Brain tumor
  • Focused ultrasound
  • Glioma
  • Permeability

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

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