Evaluation of the In Vivo Therapeutic Effects of Radix Paeoniae Rubra Ethanol Extract with the Hypoglycemic Activities Measured from Multiple Cell-Based Assays

Chia Chuan Chang, Wei Yuan, Yun Lian Lin, Ren Shyan Liu, Yi Chen Juan, Wan Hua Sun, Huey Jen Tsay, Hsiu Chen Huang, Yu Ching Lee, Hui Kang Liu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background. Radix Paeoniae Rubra (Chi Shao) contains several phytochemicals with hypoglycemic actions. Current research aims to explore potential insulinotropic effects and long-term therapeutic efficacy of such herb against type 2 diabetes. Methods. Composition analysis for the ethanol extract (PRExt) was executed by high performance liquid chromatography. Polyphenol-enriched fraction was characterized by high pressure size exclusion chromatography. Multiple cell platforms were employed to evaluate hypoglycemic bioactivities. In animal experiments, blood glucose, the homeostasis model assessment (HOMA)-index assessment, glucose tolerance test, and in vivo glucose uptake were all measured. Additional effects of PRExt on obesity and hepatic steatosis were evaluated by serum and histological analysis. Results. PRExt provides multiple hypoglycemic effects including the enhancement of glucose-mediated insulin secretion. Pentagalloylglucose and polyphenol-enriched fraction are two insulinotropic constituents. Moreover, PRExt intraperitoneal injection causes acute hypoglycemic effects on fasted db/db mice. Oral administration of PRExt (200 mg/kg b.w.) gradually reduces blood glucose in db/db mice to the level similar to that in C57J/B6 mice after 30 days. The improvement of glucose intolerance, HOMA-index, and in vivo glucose uptake is evident in addition to the weight loss effect and attenuation of hepatic steatosis. Conclusion. PRExt is an effective antidiabetic herbal extract with multiple hypoglycemic bioactivities.

Original languageEnglish
Article number3262790
JournalEvidence-based Complementary and Alternative Medicine
Volume2016
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Paeonia
Therapeutic Uses
Hypoglycemic Agents
Ethanol
Polyphenols
Glucose
Blood Glucose
Homeostasis
Glucose Intolerance
Liver
Phytochemicals
Glucose Tolerance Test
Intraperitoneal Injections
Type 2 Diabetes Mellitus
Gel Chromatography
Oral Administration
Weight Loss
Obesity
High Pressure Liquid Chromatography
Insulin

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Evaluation of the In Vivo Therapeutic Effects of Radix Paeoniae Rubra Ethanol Extract with the Hypoglycemic Activities Measured from Multiple Cell-Based Assays. / Chang, Chia Chuan; Yuan, Wei; Lin, Yun Lian; Liu, Ren Shyan; Juan, Yi Chen; Sun, Wan Hua; Tsay, Huey Jen; Huang, Hsiu Chen; Lee, Yu Ching; Liu, Hui Kang.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2016, 3262790, 01.01.2016.

Research output: Contribution to journalArticle

Chang, Chia Chuan ; Yuan, Wei ; Lin, Yun Lian ; Liu, Ren Shyan ; Juan, Yi Chen ; Sun, Wan Hua ; Tsay, Huey Jen ; Huang, Hsiu Chen ; Lee, Yu Ching ; Liu, Hui Kang. / Evaluation of the In Vivo Therapeutic Effects of Radix Paeoniae Rubra Ethanol Extract with the Hypoglycemic Activities Measured from Multiple Cell-Based Assays. In: Evidence-based Complementary and Alternative Medicine. 2016 ; Vol. 2016.
@article{751a4bfebac8403a963d82de4355b2c2,
title = "Evaluation of the In Vivo Therapeutic Effects of Radix Paeoniae Rubra Ethanol Extract with the Hypoglycemic Activities Measured from Multiple Cell-Based Assays",
abstract = "Background. Radix Paeoniae Rubra (Chi Shao) contains several phytochemicals with hypoglycemic actions. Current research aims to explore potential insulinotropic effects and long-term therapeutic efficacy of such herb against type 2 diabetes. Methods. Composition analysis for the ethanol extract (PRExt) was executed by high performance liquid chromatography. Polyphenol-enriched fraction was characterized by high pressure size exclusion chromatography. Multiple cell platforms were employed to evaluate hypoglycemic bioactivities. In animal experiments, blood glucose, the homeostasis model assessment (HOMA)-index assessment, glucose tolerance test, and in vivo glucose uptake were all measured. Additional effects of PRExt on obesity and hepatic steatosis were evaluated by serum and histological analysis. Results. PRExt provides multiple hypoglycemic effects including the enhancement of glucose-mediated insulin secretion. Pentagalloylglucose and polyphenol-enriched fraction are two insulinotropic constituents. Moreover, PRExt intraperitoneal injection causes acute hypoglycemic effects on fasted db/db mice. Oral administration of PRExt (200 mg/kg b.w.) gradually reduces blood glucose in db/db mice to the level similar to that in C57J/B6 mice after 30 days. The improvement of glucose intolerance, HOMA-index, and in vivo glucose uptake is evident in addition to the weight loss effect and attenuation of hepatic steatosis. Conclusion. PRExt is an effective antidiabetic herbal extract with multiple hypoglycemic bioactivities.",
author = "Chang, {Chia Chuan} and Wei Yuan and Lin, {Yun Lian} and Liu, {Ren Shyan} and Juan, {Yi Chen} and Sun, {Wan Hua} and Tsay, {Huey Jen} and Huang, {Hsiu Chen} and Lee, {Yu Ching} and Liu, {Hui Kang}",
year = "2016",
month = "1",
day = "1",
doi = "10.1155/2016/3262790",
language = "English",
volume = "2016",
journal = "Evidence-based Complementary and Alternative Medicine",
issn = "1741-427X",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Evaluation of the In Vivo Therapeutic Effects of Radix Paeoniae Rubra Ethanol Extract with the Hypoglycemic Activities Measured from Multiple Cell-Based Assays

AU - Chang, Chia Chuan

AU - Yuan, Wei

AU - Lin, Yun Lian

AU - Liu, Ren Shyan

AU - Juan, Yi Chen

AU - Sun, Wan Hua

AU - Tsay, Huey Jen

AU - Huang, Hsiu Chen

AU - Lee, Yu Ching

AU - Liu, Hui Kang

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Background. Radix Paeoniae Rubra (Chi Shao) contains several phytochemicals with hypoglycemic actions. Current research aims to explore potential insulinotropic effects and long-term therapeutic efficacy of such herb against type 2 diabetes. Methods. Composition analysis for the ethanol extract (PRExt) was executed by high performance liquid chromatography. Polyphenol-enriched fraction was characterized by high pressure size exclusion chromatography. Multiple cell platforms were employed to evaluate hypoglycemic bioactivities. In animal experiments, blood glucose, the homeostasis model assessment (HOMA)-index assessment, glucose tolerance test, and in vivo glucose uptake were all measured. Additional effects of PRExt on obesity and hepatic steatosis were evaluated by serum and histological analysis. Results. PRExt provides multiple hypoglycemic effects including the enhancement of glucose-mediated insulin secretion. Pentagalloylglucose and polyphenol-enriched fraction are two insulinotropic constituents. Moreover, PRExt intraperitoneal injection causes acute hypoglycemic effects on fasted db/db mice. Oral administration of PRExt (200 mg/kg b.w.) gradually reduces blood glucose in db/db mice to the level similar to that in C57J/B6 mice after 30 days. The improvement of glucose intolerance, HOMA-index, and in vivo glucose uptake is evident in addition to the weight loss effect and attenuation of hepatic steatosis. Conclusion. PRExt is an effective antidiabetic herbal extract with multiple hypoglycemic bioactivities.

AB - Background. Radix Paeoniae Rubra (Chi Shao) contains several phytochemicals with hypoglycemic actions. Current research aims to explore potential insulinotropic effects and long-term therapeutic efficacy of such herb against type 2 diabetes. Methods. Composition analysis for the ethanol extract (PRExt) was executed by high performance liquid chromatography. Polyphenol-enriched fraction was characterized by high pressure size exclusion chromatography. Multiple cell platforms were employed to evaluate hypoglycemic bioactivities. In animal experiments, blood glucose, the homeostasis model assessment (HOMA)-index assessment, glucose tolerance test, and in vivo glucose uptake were all measured. Additional effects of PRExt on obesity and hepatic steatosis were evaluated by serum and histological analysis. Results. PRExt provides multiple hypoglycemic effects including the enhancement of glucose-mediated insulin secretion. Pentagalloylglucose and polyphenol-enriched fraction are two insulinotropic constituents. Moreover, PRExt intraperitoneal injection causes acute hypoglycemic effects on fasted db/db mice. Oral administration of PRExt (200 mg/kg b.w.) gradually reduces blood glucose in db/db mice to the level similar to that in C57J/B6 mice after 30 days. The improvement of glucose intolerance, HOMA-index, and in vivo glucose uptake is evident in addition to the weight loss effect and attenuation of hepatic steatosis. Conclusion. PRExt is an effective antidiabetic herbal extract with multiple hypoglycemic bioactivities.

UR - http://www.scopus.com/inward/record.url?scp=85006164670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85006164670&partnerID=8YFLogxK

U2 - 10.1155/2016/3262790

DO - 10.1155/2016/3262790

M3 - Article

VL - 2016

JO - Evidence-based Complementary and Alternative Medicine

JF - Evidence-based Complementary and Alternative Medicine

SN - 1741-427X

M1 - 3262790

ER -