Evaluation of the frequencies of chromosomal aberrations in a population exposed to prolonged low dose-rate 60Co γ-irradiation

Wanhua A. Hsieh, Chyiyao Ni, Jeng Jong Hwang, Jye Siung Fang, Shuan Pei Lin, Yiping A. Lin, T. W. Huang, Wushou P. Chang

Research output: Contribution to journalArticle

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Abstract

Purpose: Chromosomal aberration analysis in peripheral blood lymphocytes was performed to evaluate late cytogenetic effects of long-term low dose-rate γ-irradiation exposure among students and residents exposed in radiocontaminated buildings. Materials and methods: Blood samples were taken from 1913 subjects (age 17.8±13.6, mean±SD) 5-8 years after their relocation from radioactive environments as well as from 176 non-exposed subjects (age 29.6±11.9) from the local community. Their lymphocytes were cultured for 48 h and metaphase spreads were prepared. A total of 208 900 metaphases were analysed for different types of chromosomal aberrations. Results: Relatively higher frequencies of translocations (2.1 × 10-3), rings (0.6 × 10-3) and dicentrics (0.6 × 10-3) were noted in the exposed population as compared with the non-exposed reference populations. Moreover, 356 (78.6%) of the 453 inversions were found on 14q11.2q32 in the exposed population. Among 392 well-demonstrated translocations, 167 (42.6%) and 175 (44.6%) occurred in chromosomes 7 and 14, respectively, while 139 (35.5%) occurred as t(7;14). In particular, the aberrations t(7;14)(p13;q11.2), t(7;14)(p15;q11.2) and t(7;14)(q36;q11.2) were the most prevalent, occurring with frequencies of 19 (13.7%), 20 (14.4%) and 27 (19.4%), respectively. In these, 3205 breakpoints were documented, with chromosomes 7, 9 and 14 shown to carry significantly higher frequencies of breakpoints than expected (χ2-test, p

Original languageEnglish
Pages (from-to)625-633
Number of pages9
JournalInternational Journal of Radiation Biology
Volume78
Issue number7
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

chromosome aberrations
at-risk population
Aberrations
Chromosome Aberrations
Dosimetry
Chromosomes, Human, Pair 14
aberration
Chromosomes, Human, Pair 7
Lymphocytes
irradiation
lymphocytes
chromosomes
Irradiation
Metaphase
Chromosomes
metaphase
dosage
blood
evaluation
Blood

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Evaluation of the frequencies of chromosomal aberrations in a population exposed to prolonged low dose-rate 60Co γ-irradiation. / Hsieh, Wanhua A.; Ni, Chyiyao; Hwang, Jeng Jong; Fang, Jye Siung; Lin, Shuan Pei; Lin, Yiping A.; Huang, T. W.; Chang, Wushou P.

In: International Journal of Radiation Biology, Vol. 78, No. 7, 2002, p. 625-633.

Research output: Contribution to journalArticle

Hsieh, Wanhua A. ; Ni, Chyiyao ; Hwang, Jeng Jong ; Fang, Jye Siung ; Lin, Shuan Pei ; Lin, Yiping A. ; Huang, T. W. ; Chang, Wushou P. / Evaluation of the frequencies of chromosomal aberrations in a population exposed to prolonged low dose-rate 60Co γ-irradiation. In: International Journal of Radiation Biology. 2002 ; Vol. 78, No. 7. pp. 625-633.
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abstract = "Purpose: Chromosomal aberration analysis in peripheral blood lymphocytes was performed to evaluate late cytogenetic effects of long-term low dose-rate γ-irradiation exposure among students and residents exposed in radiocontaminated buildings. Materials and methods: Blood samples were taken from 1913 subjects (age 17.8±13.6, mean±SD) 5-8 years after their relocation from radioactive environments as well as from 176 non-exposed subjects (age 29.6±11.9) from the local community. Their lymphocytes were cultured for 48 h and metaphase spreads were prepared. A total of 208 900 metaphases were analysed for different types of chromosomal aberrations. Results: Relatively higher frequencies of translocations (2.1 × 10-3), rings (0.6 × 10-3) and dicentrics (0.6 × 10-3) were noted in the exposed population as compared with the non-exposed reference populations. Moreover, 356 (78.6{\%}) of the 453 inversions were found on 14q11.2q32 in the exposed population. Among 392 well-demonstrated translocations, 167 (42.6{\%}) and 175 (44.6{\%}) occurred in chromosomes 7 and 14, respectively, while 139 (35.5{\%}) occurred as t(7;14). In particular, the aberrations t(7;14)(p13;q11.2), t(7;14)(p15;q11.2) and t(7;14)(q36;q11.2) were the most prevalent, occurring with frequencies of 19 (13.7{\%}), 20 (14.4{\%}) and 27 (19.4{\%}), respectively. In these, 3205 breakpoints were documented, with chromosomes 7, 9 and 14 shown to carry significantly higher frequencies of breakpoints than expected (χ2-test, p",
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T1 - Evaluation of the frequencies of chromosomal aberrations in a population exposed to prolonged low dose-rate 60Co γ-irradiation

AU - Hsieh, Wanhua A.

AU - Ni, Chyiyao

AU - Hwang, Jeng Jong

AU - Fang, Jye Siung

AU - Lin, Shuan Pei

AU - Lin, Yiping A.

AU - Huang, T. W.

AU - Chang, Wushou P.

PY - 2002

Y1 - 2002

N2 - Purpose: Chromosomal aberration analysis in peripheral blood lymphocytes was performed to evaluate late cytogenetic effects of long-term low dose-rate γ-irradiation exposure among students and residents exposed in radiocontaminated buildings. Materials and methods: Blood samples were taken from 1913 subjects (age 17.8±13.6, mean±SD) 5-8 years after their relocation from radioactive environments as well as from 176 non-exposed subjects (age 29.6±11.9) from the local community. Their lymphocytes were cultured for 48 h and metaphase spreads were prepared. A total of 208 900 metaphases were analysed for different types of chromosomal aberrations. Results: Relatively higher frequencies of translocations (2.1 × 10-3), rings (0.6 × 10-3) and dicentrics (0.6 × 10-3) were noted in the exposed population as compared with the non-exposed reference populations. Moreover, 356 (78.6%) of the 453 inversions were found on 14q11.2q32 in the exposed population. Among 392 well-demonstrated translocations, 167 (42.6%) and 175 (44.6%) occurred in chromosomes 7 and 14, respectively, while 139 (35.5%) occurred as t(7;14). In particular, the aberrations t(7;14)(p13;q11.2), t(7;14)(p15;q11.2) and t(7;14)(q36;q11.2) were the most prevalent, occurring with frequencies of 19 (13.7%), 20 (14.4%) and 27 (19.4%), respectively. In these, 3205 breakpoints were documented, with chromosomes 7, 9 and 14 shown to carry significantly higher frequencies of breakpoints than expected (χ2-test, p

AB - Purpose: Chromosomal aberration analysis in peripheral blood lymphocytes was performed to evaluate late cytogenetic effects of long-term low dose-rate γ-irradiation exposure among students and residents exposed in radiocontaminated buildings. Materials and methods: Blood samples were taken from 1913 subjects (age 17.8±13.6, mean±SD) 5-8 years after their relocation from radioactive environments as well as from 176 non-exposed subjects (age 29.6±11.9) from the local community. Their lymphocytes were cultured for 48 h and metaphase spreads were prepared. A total of 208 900 metaphases were analysed for different types of chromosomal aberrations. Results: Relatively higher frequencies of translocations (2.1 × 10-3), rings (0.6 × 10-3) and dicentrics (0.6 × 10-3) were noted in the exposed population as compared with the non-exposed reference populations. Moreover, 356 (78.6%) of the 453 inversions were found on 14q11.2q32 in the exposed population. Among 392 well-demonstrated translocations, 167 (42.6%) and 175 (44.6%) occurred in chromosomes 7 and 14, respectively, while 139 (35.5%) occurred as t(7;14). In particular, the aberrations t(7;14)(p13;q11.2), t(7;14)(p15;q11.2) and t(7;14)(q36;q11.2) were the most prevalent, occurring with frequencies of 19 (13.7%), 20 (14.4%) and 27 (19.4%), respectively. In these, 3205 breakpoints were documented, with chromosomes 7, 9 and 14 shown to carry significantly higher frequencies of breakpoints than expected (χ2-test, p

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