Evaluation of the antioxidant and melanogenesis inhibitory properties of Pracparatum Mungo (Lu-Do Huang)

Yu Yu Kao, Tien Fu Chuang, Shiou Huei Chao, Jo Hsuan Yang, Yu Chuan Lin, Hui Yu Huang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Pracparatum mungo (Lu-Do Huang) is a traditional Chinese functional medicine made from the natural fermentation of mung bean (L Dòu) mixed with other Chinese medicines. It has been recognized as having liver protecting and detoxifying effects. As mung beans have been verified to possess anti-inflammatory, antioxidant, antipyretic, and whitening actions, the present research utilized thein vitro , ex vivo , and in vivo experimental models to investigate the antioxidant and melanin inhibiting effects of P. mungo on the skin. The in vitro experiment revealed that P. mungo methanol extract (PMME) and P. mungo ethanol extract (PMEE) possess the capacity to clear α,α-diphenyl-2-picrylhydrazyl (DPPH) radicals and inhibit tyrosinase activity. The ex vivo experiment indicated that PMEE can promote the growth of MDCK cells and increase the enzymatic activities of superoxide dismutase (SOD) and catalase in MDCK cells. On the other hand, PMME and PMEE can suppress the proliferation of A375 cells, and PMEE can reduce the enzymatic activities of SOD and catalase in A375 cells. The in vivo results showed that P. mungo can enhance the enzymatic performance of SOD, Catalase, and glutathione peroxidase (GPx) in the liver. The results also showed that P. mungo has antioxidant characteristics and can inhibit tyrosinase activity, thereby promoting the growth of skin tissues and suppressing the proliferation of A375 cells, and thus enhancing the effects that the antioxidant enzymatic performance has on the liver. These results can be applied in the development of tyrosinase inhibitors or antioxidants used for the inhibition of melanin biosynthesis or for auto-oxidation in further industrial applications, particularly those relating to functional food or cosmetic compositions.

Original languageEnglish
Pages (from-to)163-170
Number of pages8
JournalJournal of Traditional and Complementary Medicine
Volume3
Issue number3
DOIs
Publication statusPublished - Jan 1 2013
Externally publishedYes

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Antioxidants
Monophenol Monooxygenase
Ethanol
Catalase
Superoxide Dismutase
Madin Darby Canine Kidney Cells
Melanins
Methanol
Liver
Cell Proliferation
Antipyretics
Skin
Functional Food
Health Services Research
Chinese Traditional Medicine
Glutathione Peroxidase
Cosmetics
Fermentation
Anti-Inflammatory Agents
Theoretical Models

Keywords

  • Anti-melanogenesis
  • Antioxidant
  • Pracparatum mungo
  • Tyrosinase inhibitor

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Evaluation of the antioxidant and melanogenesis inhibitory properties of Pracparatum Mungo (Lu-Do Huang). / Kao, Yu Yu; Chuang, Tien Fu; Chao, Shiou Huei; Yang, Jo Hsuan; Lin, Yu Chuan; Huang, Hui Yu.

In: Journal of Traditional and Complementary Medicine, Vol. 3, No. 3, 01.01.2013, p. 163-170.

Research output: Contribution to journalArticle

Kao, Yu Yu ; Chuang, Tien Fu ; Chao, Shiou Huei ; Yang, Jo Hsuan ; Lin, Yu Chuan ; Huang, Hui Yu. / Evaluation of the antioxidant and melanogenesis inhibitory properties of Pracparatum Mungo (Lu-Do Huang). In: Journal of Traditional and Complementary Medicine. 2013 ; Vol. 3, No. 3. pp. 163-170.
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