Evaluation of the antibacterial potential of liquid and vapor phase phenolic essential oil compounds against oral microorganisms

Tong Hong Wang, Shih-Min Hsia, Chi-Hao Wu, Shun Yao Ko, Michael Yuanchien Chen, Yin Hua Shih, Tzong Ming Shieh, Li Chuan Chuang, Yi Ching Wu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The aim of the present study was to determine the antibacterial activities of the phenolic essential oil (EO) compounds hinokitiol, carvacrol, thymol, and menthol against oral pathogens. Aggregatibacter actinomycetemcomitans, Streptococcus mutans, Methicillin-resistant Staphylococcus aureus (MRSA), and Escherichia. coli were used in this study. The minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), bacterial growth curves, temperature and pH stabilities, and synergistic effects of the liquid and vapor EO compounds were tested. The MIC/MBC of the EO compounds, ranging from the strongest to weakest, were hinokitiol (40-60 μg/mL/40-100 μg/mL), thymol (100-200 μg/mL/200-400 μg/mL), carvacrol (200-400 μg/mL/200-600 μg/mL), and menthol (500-more than 2500 μg/mL/1000-more than 2500 μg/mL). The antibacterial activities of the four EO phenolic compound based on the agar diffusion test and bacterial growth curves showed that the four EO phenolic compounds were stable under different temperatures for 24 h, but the thymol activity decreased when the temperature was higher than 80°C. The combination of liquid carvacrol with thymol did not show any synergistic effects. The activities of the vaporous carvacrol and thymol were inhibited by the presence of water. Continual violent shaking during culture enhanced the activity of menthol. Both liquid and vaporous hinokitiol were stable at different temperatures and pH conditions. The combination of vaporous hinokitiol with zinc oxide did not show synergistic effects. These results showed that the liquid and vapor phases of hinokitiol have strong anti-oral bacteria abilities. Hinokitiol has the potential to be applied in oral health care products, dental materials, and infection controls to exert antimicrobial activity.

Original languageEnglish
Article numbere0163147
JournalPLoS One
Volume11
Issue number9
DOIs
Publication statusPublished - Sep 1 2016

Fingerprint

thymol
Thymol
Volatile Oils
vapors
Microorganisms
carvacrol
mouth
essential oils
menthol
Vapors
Menthol
microorganisms
liquids
Liquids
minimum inhibitory concentration
Temperature
Microbial Sensitivity Tests
microbial growth
phenolic compounds
Actinobacillus actinomycetemcomitans

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Evaluation of the antibacterial potential of liquid and vapor phase phenolic essential oil compounds against oral microorganisms. / Wang, Tong Hong; Hsia, Shih-Min; Wu, Chi-Hao; Ko, Shun Yao; Yuanchien Chen, Michael; Shih, Yin Hua; Shieh, Tzong Ming; Chuang, Li Chuan; Wu, Yi Ching.

In: PLoS One, Vol. 11, No. 9, e0163147, 01.09.2016.

Research output: Contribution to journalArticle

Wang, Tong Hong ; Hsia, Shih-Min ; Wu, Chi-Hao ; Ko, Shun Yao ; Yuanchien Chen, Michael ; Shih, Yin Hua ; Shieh, Tzong Ming ; Chuang, Li Chuan ; Wu, Yi Ching. / Evaluation of the antibacterial potential of liquid and vapor phase phenolic essential oil compounds against oral microorganisms. In: PLoS One. 2016 ; Vol. 11, No. 9.
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