Evaluation of recombinant fructose-1,6-bisphosphate aldolase ELISA test for the diagnosis of Schistosoma japonicum in water buffaloes

Shih Y. Peng, Kin M. Lee, John Chin Tsaihong, Po Ching Cheng, Ping Chin Fan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fructose-1,6-bisphosphate aldolase (FBPA) is an ubiquitous enzyme essential for glycolysis, gluconeogenesis and the Calvin cycle. It has been demonstrated to induce immune responses and to be useful in the immunodiagnosis of malaria. In this study, FBPA was cloned from the adult worms of Schistosoma japonicum and tested as an antigen for the diagnosis of S. japonicum infection in water buffaloes. Enzyme-linked immunosorbent assay (ELISA) was performed on the sera from 32 infected water buffaloes and 20 negative controls using the recombinant FBPA protein or soluble worm antigen preparation (SWAP) as an antigen. The OD cut-off values were determined to be 0.57 with 100% specificity and 100% sensitivity for the FBPA ELISA and 1.13 with 93.8% specificity and 95.0% sensitivity for the SWAP ELISA. These findings indicate that the recombinant FBPA of S. japonicum should be an useful diagnostic tool for the detection of antibodies against S. japonicum.

Original languageEnglish
Pages (from-to)527-533
Number of pages7
JournalResearch in Veterinary Science
Volume85
Issue number3
DOIs
Publication statusPublished - Dec 2008
Externally publishedYes

Fingerprint

Schistosoma japonicum
fructose-bisphosphate aldolase
Fructose-Bisphosphate Aldolase
Buffaloes
buffaloes
fructose
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
antigens
Antigens
testing
Sensitivity and Specificity
Immunologic Tests
Gluconeogenesis
gluconeogenesis
Calvin cycle
serodiagnosis
Photosynthesis
glycolysis
Glycolysis

Keywords

  • ELISA
  • Fructose-1,6-bisphosphate aldolase
  • Schistosoma japonicum

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Evaluation of recombinant fructose-1,6-bisphosphate aldolase ELISA test for the diagnosis of Schistosoma japonicum in water buffaloes. / Peng, Shih Y.; Lee, Kin M.; Tsaihong, John Chin; Cheng, Po Ching; Fan, Ping Chin.

In: Research in Veterinary Science, Vol. 85, No. 3, 12.2008, p. 527-533.

Research output: Contribution to journalArticle

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