Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles

Tsi Shu Huang, Yung Ching Liu, Wen Kuei Huang, Chii Shiang Chen, Hui Zin Tu, Deh Lin Cheng

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6 Citations (Scopus)


Polymerase chain reaction with restriction enzyme analysis (PRA) was first tested on 15 reference strains and 50 subcultured clinical isolates of mycobacteria according to the reference algorithm by Telenti et al [1]. Next, we evaluated the application of this method to 108 isolates from liquid media (BACTEC 12B). Of them, 15 M. tuberculosis complex and 81 mycobacteria other than tuberculosis (MOTT) had comparable results with both PRA and the BACTEC 460 TB systems. However, seven M. tuberculosis complex and three potentially pathogenic MOTT were identified by PRA rather than the BACTEC TB system. PRA seems to be an efficient method for the identification of mycobacteria to the species level and a good aid to detect potentially pathogenic mycobacteria, especially in mixed mycobacterial cultures.

Original languageEnglish
Pages (from-to)530-535
Number of pages6
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Issue number7
Publication statusPublished - Jul 1996
Externally publishedYes



  • BACTEC TB system
  • Mycobacteria
  • Polymerase chain reaction
  • Restriction enzyme analysis

ASJC Scopus subject areas

  • Medicine(all)

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