Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles

Tsi Shu Huang, Yung Ching Liu, Wen Kuei Huang, Chii Shiang Chen, Hui Zin Tu, Deh Lin Cheng

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Polymerase chain reaction with restriction enzyme analysis (PRA) was first tested on 15 reference strains and 50 subcultured clinical isolates of mycobacteria according to the reference algorithm by Telenti et al [1]. Next, we evaluated the application of this method to 108 isolates from liquid media (BACTEC 12B). Of them, 15 M. tuberculosis complex and 81 mycobacteria other than tuberculosis (MOTT) had comparable results with both PRA and the BACTEC 460 TB systems. However, seven M. tuberculosis complex and three potentially pathogenic MOTT were identified by PRA rather than the BACTEC TB system. PRA seems to be an efficient method for the identification of mycobacteria to the species level and a good aid to detect potentially pathogenic mycobacteria, especially in mixed mycobacterial cultures.

Original languageEnglish
Pages (from-to)530-535
Number of pages6
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume95
Issue number7
Publication statusPublished - Jul 1996
Externally publishedYes

Fingerprint

Restriction Mapping
Mycobacterium
Mycobacterium tuberculosis
Polymerase Chain Reaction
Tuberculosis

Keywords

  • BACTEC TB system
  • Mycobacteria
  • Polymerase chain reaction
  • Restriction enzyme analysis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles. / Huang, Tsi Shu; Liu, Yung Ching; Huang, Wen Kuei; Chen, Chii Shiang; Tu, Hui Zin; Cheng, Deh Lin.

In: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 95, No. 7, 07.1996, p. 530-535.

Research output: Contribution to journalArticle

Huang, Tsi Shu ; Liu, Yung Ching ; Huang, Wen Kuei ; Chen, Chii Shiang ; Tu, Hui Zin ; Cheng, Deh Lin. / Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles. In: Journal of the Formosan Medical Association = Taiwan yi zhi. 1996 ; Vol. 95, No. 7. pp. 530-535.
@article{fc63c7a807a54bec8d096be4124b373b,
title = "Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles",
abstract = "Polymerase chain reaction with restriction enzyme analysis (PRA) was first tested on 15 reference strains and 50 subcultured clinical isolates of mycobacteria according to the reference algorithm by Telenti et al [1]. Next, we evaluated the application of this method to 108 isolates from liquid media (BACTEC 12B). Of them, 15 M. tuberculosis complex and 81 mycobacteria other than tuberculosis (MOTT) had comparable results with both PRA and the BACTEC 460 TB systems. However, seven M. tuberculosis complex and three potentially pathogenic MOTT were identified by PRA rather than the BACTEC TB system. PRA seems to be an efficient method for the identification of mycobacteria to the species level and a good aid to detect potentially pathogenic mycobacteria, especially in mixed mycobacterial cultures.",
keywords = "BACTEC TB system, Mycobacteria, Polymerase chain reaction, Restriction enzyme analysis",
author = "Huang, {Tsi Shu} and Liu, {Yung Ching} and Huang, {Wen Kuei} and Chen, {Chii Shiang} and Tu, {Hui Zin} and Cheng, {Deh Lin}",
year = "1996",
month = "7",
language = "English",
volume = "95",
pages = "530--535",
journal = "Journal of the Formosan Medical Association",
issn = "0929-6646",
publisher = "Elsevier Science Publishers B.V.",
number = "7",

}

TY - JOUR

T1 - Evaluation of polymerase chain reaction-restriction enzyme analysis of mycobacteria cultured in BACTEC 12B bottles

AU - Huang, Tsi Shu

AU - Liu, Yung Ching

AU - Huang, Wen Kuei

AU - Chen, Chii Shiang

AU - Tu, Hui Zin

AU - Cheng, Deh Lin

PY - 1996/7

Y1 - 1996/7

N2 - Polymerase chain reaction with restriction enzyme analysis (PRA) was first tested on 15 reference strains and 50 subcultured clinical isolates of mycobacteria according to the reference algorithm by Telenti et al [1]. Next, we evaluated the application of this method to 108 isolates from liquid media (BACTEC 12B). Of them, 15 M. tuberculosis complex and 81 mycobacteria other than tuberculosis (MOTT) had comparable results with both PRA and the BACTEC 460 TB systems. However, seven M. tuberculosis complex and three potentially pathogenic MOTT were identified by PRA rather than the BACTEC TB system. PRA seems to be an efficient method for the identification of mycobacteria to the species level and a good aid to detect potentially pathogenic mycobacteria, especially in mixed mycobacterial cultures.

AB - Polymerase chain reaction with restriction enzyme analysis (PRA) was first tested on 15 reference strains and 50 subcultured clinical isolates of mycobacteria according to the reference algorithm by Telenti et al [1]. Next, we evaluated the application of this method to 108 isolates from liquid media (BACTEC 12B). Of them, 15 M. tuberculosis complex and 81 mycobacteria other than tuberculosis (MOTT) had comparable results with both PRA and the BACTEC 460 TB systems. However, seven M. tuberculosis complex and three potentially pathogenic MOTT were identified by PRA rather than the BACTEC TB system. PRA seems to be an efficient method for the identification of mycobacteria to the species level and a good aid to detect potentially pathogenic mycobacteria, especially in mixed mycobacterial cultures.

KW - BACTEC TB system

KW - Mycobacteria

KW - Polymerase chain reaction

KW - Restriction enzyme analysis

UR - http://www.scopus.com/inward/record.url?scp=0030184703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030184703&partnerID=8YFLogxK

M3 - Article

VL - 95

SP - 530

EP - 535

JO - Journal of the Formosan Medical Association

JF - Journal of the Formosan Medical Association

SN - 0929-6646

IS - 7

ER -