Abstract

Background/purpose The aim of this study was to investigate whether storage of extracted teeth in transportation solution for 24 hours has a negative effect on the cell density and mechanical properties of thawed pulp tissue extracted from frozen intact teeth. Material and methods Human premolars were kept in transportation solution for 0 hours or 24 hours. For each transportation time, the tested teeth were divided into two experimental groups: teeth that had been frozen in a magnetic programmed freezer (PF, n = 5) and in a traditional freezer at -20°C (TF, n = 5). The tested teeth were then stored at -150°C for 7 days. After thawing, the extracted pulp was subjected to a histological examination and mechanical testing. Results Storage in transportation solution for 24 hours had no significant negative effects on the histological or mechanical properties of the pulp tissue extracted from the cryopreserved intact teeth. The elastic modulus of pulp from the teeth that had been frozen at -20°C was significantly higher (P <0.05) in samples that had been pre-stored in transportation solution for 24 hours. Conclusion This study demonstrated that exposure to transportation solution for 24 hours followed by storage in a programmed freezer did not negatively affect the cell density or the elastic modulus of the pulp tissue.

Original languageEnglish
Pages (from-to)244-248
Number of pages5
JournalJournal of Dental Sciences
Volume9
Issue number3
DOIs
Publication statusPublished - 2014

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Bicuspid
Tooth
Elastic Modulus
Cell Count

Keywords

  • cryopreservation
  • program freezer
  • tooth

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

@article{ffbcac66e990473daf2f88ddbd3e3605,
title = "Evaluation of mechanical and histological properties of cryopreserved human premolars under short-term preservation: A preliminary study",
abstract = "Background/purpose The aim of this study was to investigate whether storage of extracted teeth in transportation solution for 24 hours has a negative effect on the cell density and mechanical properties of thawed pulp tissue extracted from frozen intact teeth. Material and methods Human premolars were kept in transportation solution for 0 hours or 24 hours. For each transportation time, the tested teeth were divided into two experimental groups: teeth that had been frozen in a magnetic programmed freezer (PF, n = 5) and in a traditional freezer at -20°C (TF, n = 5). The tested teeth were then stored at -150°C for 7 days. After thawing, the extracted pulp was subjected to a histological examination and mechanical testing. Results Storage in transportation solution for 24 hours had no significant negative effects on the histological or mechanical properties of the pulp tissue extracted from the cryopreserved intact teeth. The elastic modulus of pulp from the teeth that had been frozen at -20°C was significantly higher (P <0.05) in samples that had been pre-stored in transportation solution for 24 hours. Conclusion This study demonstrated that exposure to transportation solution for 24 hours followed by storage in a programmed freezer did not negatively affect the cell density or the elastic modulus of the pulp tissue.",
keywords = "cryopreservation, program freezer, tooth",
author = "Lin, {Shu Li} and Lee, {Sheng Yang} and Lin, {Yen Chuang} and Huang, {Yen Hua} and Yang, {Jen Chang} and Huang, {Haw Ming}",
year = "2014",
doi = "10.1016/j.jds.2013.04.010",
language = "English",
volume = "9",
pages = "244--248",
journal = "Journal of Dental Sciences",
issn = "1991-7902",
publisher = "中華牙醫學會",
number = "3",

}

TY - JOUR

T1 - Evaluation of mechanical and histological properties of cryopreserved human premolars under short-term preservation

T2 - A preliminary study

AU - Lin, Shu Li

AU - Lee, Sheng Yang

AU - Lin, Yen Chuang

AU - Huang, Yen Hua

AU - Yang, Jen Chang

AU - Huang, Haw Ming

PY - 2014

Y1 - 2014

N2 - Background/purpose The aim of this study was to investigate whether storage of extracted teeth in transportation solution for 24 hours has a negative effect on the cell density and mechanical properties of thawed pulp tissue extracted from frozen intact teeth. Material and methods Human premolars were kept in transportation solution for 0 hours or 24 hours. For each transportation time, the tested teeth were divided into two experimental groups: teeth that had been frozen in a magnetic programmed freezer (PF, n = 5) and in a traditional freezer at -20°C (TF, n = 5). The tested teeth were then stored at -150°C for 7 days. After thawing, the extracted pulp was subjected to a histological examination and mechanical testing. Results Storage in transportation solution for 24 hours had no significant negative effects on the histological or mechanical properties of the pulp tissue extracted from the cryopreserved intact teeth. The elastic modulus of pulp from the teeth that had been frozen at -20°C was significantly higher (P <0.05) in samples that had been pre-stored in transportation solution for 24 hours. Conclusion This study demonstrated that exposure to transportation solution for 24 hours followed by storage in a programmed freezer did not negatively affect the cell density or the elastic modulus of the pulp tissue.

AB - Background/purpose The aim of this study was to investigate whether storage of extracted teeth in transportation solution for 24 hours has a negative effect on the cell density and mechanical properties of thawed pulp tissue extracted from frozen intact teeth. Material and methods Human premolars were kept in transportation solution for 0 hours or 24 hours. For each transportation time, the tested teeth were divided into two experimental groups: teeth that had been frozen in a magnetic programmed freezer (PF, n = 5) and in a traditional freezer at -20°C (TF, n = 5). The tested teeth were then stored at -150°C for 7 days. After thawing, the extracted pulp was subjected to a histological examination and mechanical testing. Results Storage in transportation solution for 24 hours had no significant negative effects on the histological or mechanical properties of the pulp tissue extracted from the cryopreserved intact teeth. The elastic modulus of pulp from the teeth that had been frozen at -20°C was significantly higher (P <0.05) in samples that had been pre-stored in transportation solution for 24 hours. Conclusion This study demonstrated that exposure to transportation solution for 24 hours followed by storage in a programmed freezer did not negatively affect the cell density or the elastic modulus of the pulp tissue.

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KW - program freezer

KW - tooth

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