Evaluation of magnetic nanoparticle-labeled chondrocytes cultivated on a type II collagen–chitosan/poly(Lactic-co-glycolic) acid biphasic scaffold

Juin Yih Su, Shi Hui Chen, Yu Pin Chen, Wei Chuan Chen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Chondral or osteochondral defects are still controversial problems in orthopedics. Here, chondrocytes labeled with magnetic nanoparticles were cultivated on a biphasic, type II collagen–chitosan/poly(lactic-co-glycolic acid) scaffold in an attempt to develop cultures with trackable cells exhibiting growth, differentiation, and regeneration. Rabbit chondrocytes were labeled with magnetic nanoparticles and characterized by scanning electron microscopy (SEM), transmission electron (TEM) microscopy, and gene and protein expression analyses. The experimental results showed that the magnetic nanoparticles did not affect the phenotype of chondrocytes after cell labeling, nor were protein and gene expression affected. The biphasic type II collagen–chitosan/poly(lactic-co-glycolic) acid scaffold was characterized by SEM, and labeled chondrocytes showed a homogeneous distribution throughout the scaffold after cultivation onto the polymer. Cellular phenotype remained unaltered but with increased gene expression of type II collagen and aggrecan, as indicated by cell staining, indicating chondrogenesis. Decreased SRY-related high mobility group-box gene (Sox-9) levels of cultured chondrocytes indicated that differentiation was associated with osteogenesis. These results are encouraging for the development of techniques for trackable cartilage regeneration and osteochondral defect repair which may be applied in vivo and, eventually, in clinical trials.

Original languageEnglish
Article number87
JournalInternational journal of molecular sciences
Volume18
Issue number1
DOIs
Publication statusPublished - Jan 4 2017

Keywords

  • Biphasic scaffold
  • Chitosan
  • Chondrocytes
  • Magnetic nanoparticles
  • Poly(lactic-co-glycolic acid)
  • Type II collagen

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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