Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I

Karl B. McCann; Peter T. Gomme; John Wu; Joseph Bertolini

doi:10.1016/j.biologicals.2008.01.002

Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I

Karl B. McCann, Peter T. Gomme, John Wu, Joseph Bertolini

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.

Original language	English
Pages (from-to)	227-233
Number of pages	7
Journal	Biologicals
Volume	36
Issue number	4
DOIs	https://doi.org/10.1016/j.biologicals.2008.01.002
Publication status	Published - Jul 1 2008
Externally published	Yes

Keywords

Cohn Supernatant I
Expanded bed adsorption
Plasma
Prothrombin complex concentrate

ASJC Scopus subject areas

Immunology
Microbiology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biologicals.2008.01.002

Cite this

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title = "Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I",

abstract = "This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.",

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author = "McCann, {Karl B.} and Gomme, {Peter T.} and John Wu and Joseph Bertolini",

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AU - McCann, Karl B.

AU - Gomme, Peter T.

AU - Wu, John

AU - Bertolini, Joseph

PY - 2008/7/1

Y1 - 2008/7/1

N2 - This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.

AB - This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.

KW - Cohn Supernatant I

KW - Expanded bed adsorption

KW - Plasma

KW - Prothrombin complex concentrate

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Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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