Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I

Karl B. McCann, Peter T. Gomme, John Wu, Joseph Bertolini

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.

Original languageEnglish
Pages (from-to)227-233
Number of pages7
JournalBiologicals
Volume36
Issue number4
DOIs
Publication statusPublished - Jul 1 2008
Externally publishedYes

Keywords

  • Cohn Supernatant I
  • Expanded bed adsorption
  • Plasma
  • Prothrombin complex concentrate

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

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