Evaluation of antioxidants using an arachidonic acid-stimulated platelet electron spin resonance system

Kuo Hsien Hsieh, Jie Jen Lee, Lin Wen Lee, Joen Rong Sheu, Su Tzy Hwang, Duen Suey Chou

Research output: Contribution to journalArticle

Abstract

A novel cell-containing assay system using electron spin resonance (ESR) and spin-trapping methods was established. We used this assay system to evaluate the antioxidant activities of various dietary antioxidants including resveratrol, lycopene, quercetin and rutin. Resveratrol showed the strongest radical-scavenging activity among the compounds tested. Our results provided useful data for the establishment of a model for evaluating the antioxidant activities of natural dietary substances in cell-containing systems.

Original languageEnglish
JournalJournal of Food and Drug Analysis
Volume19
Issue number3
Publication statusPublished - Sep 2011

Fingerprint

electron paramagnetic resonance spectroscopy
resveratrol
Electron Spin Resonance Spectroscopy
arachidonic acid
Arachidonic Acid
Blood Platelets
Antioxidants
antioxidant activity
antioxidants
assays
rutin
lycopene
quercetin
Spin Trapping
Rutin
Quercetin
cells
methodology
spin trapping

Keywords

  • Arachidonic acid
  • Carbon-centered radical
  • Electron spin resonance
  • Hydroxyl radical
  • Platelets

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

Evaluation of antioxidants using an arachidonic acid-stimulated platelet electron spin resonance system. / Hsieh, Kuo Hsien; Lee, Jie Jen; Lee, Lin Wen; Sheu, Joen Rong; Hwang, Su Tzy; Chou, Duen Suey.

In: Journal of Food and Drug Analysis, Vol. 19, No. 3, 09.2011.

Research output: Contribution to journalArticle

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AU - Chou, Duen Suey

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AB - A novel cell-containing assay system using electron spin resonance (ESR) and spin-trapping methods was established. We used this assay system to evaluate the antioxidant activities of various dietary antioxidants including resveratrol, lycopene, quercetin and rutin. Resveratrol showed the strongest radical-scavenging activity among the compounds tested. Our results provided useful data for the establishment of a model for evaluating the antioxidant activities of natural dietary substances in cell-containing systems.

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