Evaluation of adhesion, proliferation, and differentiation of human adipose-derived stem cells on keratin

Che Wei Lin, Kai Chiang Yang, Nai Chen Cheng, Wei Bor Tsai, Kuo Long Lou, Jiashing Yu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Controlled adhesion and continuous growth of human adipose stem cells (hASCs) on delivery carriers are critical to cell therapy and tissue engineering. Keratin, derived from human hair, can be manipulated as a desired configuration and serve as an extracellular matrix. However, the effects of keratin on stem cells have not been fully understood. In this study, keratin-coting substrates were prepared to demonstrate the modulations of keratin matrix to the adhesion, proliferation, and differentiation of hASCs. The present results showed that keratin-coating substrates promoted hASCs adhesion, proliferation, and viability relative to those of untreated polystyrene plates. Evaluations of lineage-specific genetic markers and proteins revealed that the adipogenic, osteogenic and chondrogenic differentiations of hASCs can be successfully induced, which suggested that the stemness of hASCs was maintained when cultured on keratin-coating substrates. Relative to untreated polystyrene plates, keratin-coating increased the mRNA levels of lipoprotein lipase (LPL), peroxisome proliferator-activated receptor gamma (PPAR- γ), and CCAAT-enhancer binding protein alpha (CEBP-α) to hASCs, which reveals an improvement in adipogenic differentiation. Likewise, keratin also upregulated osteogenic markers such as type I collagen, alpha 1 (COL1A1), runt-related transcription factor 2 (RUNX2), and vitamin D receptor (VDR) to hASCs. Similarly, the chondrogenic marker SRY-box 9 (SOX9) was improved to hASCs on keratin-coating. The combination of hASCs with keratin shall be promising in the application of tissue repair to regenerative medicine.

Original languageEnglish
Article number40
JournalJournal of Polymer Research
Volume25
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

Keratin
Keratins
Stem cells
Adhesion
Coatings
Polystyrenes
Substrates
CCAAT-Enhancer-Binding Protein-alpha
Lipoproteins
Calcitriol Receptors
Peroxisome Proliferator-Activated Receptors
Transcription factors
Lipoprotein Lipase
PPAR gamma
Vitamins
Cell adhesion
Lipases
Tissue engineering
Collagen
Repair

Keywords

  • Differentiation
  • Human adipose stem cell
  • Keratin

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

Cite this

Evaluation of adhesion, proliferation, and differentiation of human adipose-derived stem cells on keratin. / Lin, Che Wei; Yang, Kai Chiang; Cheng, Nai Chen; Tsai, Wei Bor; Lou, Kuo Long; Yu, Jiashing.

In: Journal of Polymer Research, Vol. 25, No. 2, 40, 01.02.2018.

Research output: Contribution to journalArticle

Lin, Che Wei ; Yang, Kai Chiang ; Cheng, Nai Chen ; Tsai, Wei Bor ; Lou, Kuo Long ; Yu, Jiashing. / Evaluation of adhesion, proliferation, and differentiation of human adipose-derived stem cells on keratin. In: Journal of Polymer Research. 2018 ; Vol. 25, No. 2.
@article{cbc5a5ce237845c4a535f9af50205606,
title = "Evaluation of adhesion, proliferation, and differentiation of human adipose-derived stem cells on keratin",
abstract = "Controlled adhesion and continuous growth of human adipose stem cells (hASCs) on delivery carriers are critical to cell therapy and tissue engineering. Keratin, derived from human hair, can be manipulated as a desired configuration and serve as an extracellular matrix. However, the effects of keratin on stem cells have not been fully understood. In this study, keratin-coting substrates were prepared to demonstrate the modulations of keratin matrix to the adhesion, proliferation, and differentiation of hASCs. The present results showed that keratin-coating substrates promoted hASCs adhesion, proliferation, and viability relative to those of untreated polystyrene plates. Evaluations of lineage-specific genetic markers and proteins revealed that the adipogenic, osteogenic and chondrogenic differentiations of hASCs can be successfully induced, which suggested that the stemness of hASCs was maintained when cultured on keratin-coating substrates. Relative to untreated polystyrene plates, keratin-coating increased the mRNA levels of lipoprotein lipase (LPL), peroxisome proliferator-activated receptor gamma (PPAR- γ), and CCAAT-enhancer binding protein alpha (CEBP-α) to hASCs, which reveals an improvement in adipogenic differentiation. Likewise, keratin also upregulated osteogenic markers such as type I collagen, alpha 1 (COL1A1), runt-related transcription factor 2 (RUNX2), and vitamin D receptor (VDR) to hASCs. Similarly, the chondrogenic marker SRY-box 9 (SOX9) was improved to hASCs on keratin-coating. The combination of hASCs with keratin shall be promising in the application of tissue repair to regenerative medicine.",
keywords = "Differentiation, Human adipose stem cell, Keratin",
author = "Lin, {Che Wei} and Yang, {Kai Chiang} and Cheng, {Nai Chen} and Tsai, {Wei Bor} and Lou, {Kuo Long} and Jiashing Yu",
year = "2018",
month = "2",
day = "1",
doi = "10.1007/s10965-018-1446-1",
language = "English",
volume = "25",
journal = "Journal of Polymer Research",
issn = "1022-9760",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Evaluation of adhesion, proliferation, and differentiation of human adipose-derived stem cells on keratin

AU - Lin, Che Wei

AU - Yang, Kai Chiang

AU - Cheng, Nai Chen

AU - Tsai, Wei Bor

AU - Lou, Kuo Long

AU - Yu, Jiashing

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Controlled adhesion and continuous growth of human adipose stem cells (hASCs) on delivery carriers are critical to cell therapy and tissue engineering. Keratin, derived from human hair, can be manipulated as a desired configuration and serve as an extracellular matrix. However, the effects of keratin on stem cells have not been fully understood. In this study, keratin-coting substrates were prepared to demonstrate the modulations of keratin matrix to the adhesion, proliferation, and differentiation of hASCs. The present results showed that keratin-coating substrates promoted hASCs adhesion, proliferation, and viability relative to those of untreated polystyrene plates. Evaluations of lineage-specific genetic markers and proteins revealed that the adipogenic, osteogenic and chondrogenic differentiations of hASCs can be successfully induced, which suggested that the stemness of hASCs was maintained when cultured on keratin-coating substrates. Relative to untreated polystyrene plates, keratin-coating increased the mRNA levels of lipoprotein lipase (LPL), peroxisome proliferator-activated receptor gamma (PPAR- γ), and CCAAT-enhancer binding protein alpha (CEBP-α) to hASCs, which reveals an improvement in adipogenic differentiation. Likewise, keratin also upregulated osteogenic markers such as type I collagen, alpha 1 (COL1A1), runt-related transcription factor 2 (RUNX2), and vitamin D receptor (VDR) to hASCs. Similarly, the chondrogenic marker SRY-box 9 (SOX9) was improved to hASCs on keratin-coating. The combination of hASCs with keratin shall be promising in the application of tissue repair to regenerative medicine.

AB - Controlled adhesion and continuous growth of human adipose stem cells (hASCs) on delivery carriers are critical to cell therapy and tissue engineering. Keratin, derived from human hair, can be manipulated as a desired configuration and serve as an extracellular matrix. However, the effects of keratin on stem cells have not been fully understood. In this study, keratin-coting substrates were prepared to demonstrate the modulations of keratin matrix to the adhesion, proliferation, and differentiation of hASCs. The present results showed that keratin-coating substrates promoted hASCs adhesion, proliferation, and viability relative to those of untreated polystyrene plates. Evaluations of lineage-specific genetic markers and proteins revealed that the adipogenic, osteogenic and chondrogenic differentiations of hASCs can be successfully induced, which suggested that the stemness of hASCs was maintained when cultured on keratin-coating substrates. Relative to untreated polystyrene plates, keratin-coating increased the mRNA levels of lipoprotein lipase (LPL), peroxisome proliferator-activated receptor gamma (PPAR- γ), and CCAAT-enhancer binding protein alpha (CEBP-α) to hASCs, which reveals an improvement in adipogenic differentiation. Likewise, keratin also upregulated osteogenic markers such as type I collagen, alpha 1 (COL1A1), runt-related transcription factor 2 (RUNX2), and vitamin D receptor (VDR) to hASCs. Similarly, the chondrogenic marker SRY-box 9 (SOX9) was improved to hASCs on keratin-coating. The combination of hASCs with keratin shall be promising in the application of tissue repair to regenerative medicine.

KW - Differentiation

KW - Human adipose stem cell

KW - Keratin

UR - http://www.scopus.com/inward/record.url?scp=85045340852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045340852&partnerID=8YFLogxK

U2 - 10.1007/s10965-018-1446-1

DO - 10.1007/s10965-018-1446-1

M3 - Article

AN - SCOPUS:85045340852

VL - 25

JO - Journal of Polymer Research

JF - Journal of Polymer Research

SN - 1022-9760

IS - 2

M1 - 40

ER -