Ethyl acetate fraction of adlay bran ethanolic extract inhibits oncogene expression and suppresses DMH-induced preneoplastic lesions of the colon in F344 rats through an anti-inflammatory pathway

Cheng Pei Chung, Hsin Yi Hsu, Din Wen Huang, Hsing Hua Hsu, Ju Tsui Lin, Chun Kuang Shih, Wenchang Chiang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) is a grass crop and was reported to possess antiinflammatory activity and an antiproliferative effect in cancer cell lines. The purpose of this study was to evaluate the effects of the ethyl acetate fraction of an adlay bran ethanolic extract (ABE-Ea) on colon carcinogenesis in an animal model and investigate its mechanism. Male F344 rats received 1,2-dimethylhydrazine (DMH) and consumed different doses of ABE-Ea. The medium-dose group (17.28 mg of ABE-Ea/day) exhibited the best suppressive effect on colon carcinogenesis and prevented preneoplastic mucin-depleted foci (MDF) formation. Moreover, RAS and Ets2 oncogenes were significantly down-regulated in this group compared to the negative control group, whereas Wee 7, a gene involved in the cell cycle, was up-regulated. Cyclooxygenase-2 (COX-2) protein expression was significantly suppressed in all colons receiving the ABE-Ea, indicating that ABE-Ea delayed carcinogenesis by suppressing chronic inflammation. ABE-Ea included considerable a proportion of phenolic compounds, and ferulic acid was the major phenolic acid (5206 μg/g ABE-Ea) on the basis of HPLC analysis. Results from this study suggest that ABE-Ea suppressed DMH-indued preneoplastic lesions of the colon in F344 rats and that ferulic acid may be one of the active compounds.

Original languageEnglish
Pages (from-to)7616-7623
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number13
DOIs
Publication statusPublished - Jul 14 2010

Fingerprint

ferulic acid
Coix lacryma-jobi
1,2-dimethylhydrazine
1,2-Dimethylhydrazine
lesions (plant)
oncogenes
Inbred F344 Rats
bran
Oncogenes
colon
Rats
Colon
Anti-Inflammatory Agents
Cells
Carcinogenesis
rats
extracts
Mucins
Cyclooxygenase 2
Crops

Keywords

  • Adlay bran
  • Cyclooxygenase-2 (cox-2)
  • Mucin-depleted foci (mdf)
  • RAS
  • Wee1

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Medicine(all)

Cite this

Ethyl acetate fraction of adlay bran ethanolic extract inhibits oncogene expression and suppresses DMH-induced preneoplastic lesions of the colon in F344 rats through an anti-inflammatory pathway. / Chung, Cheng Pei; Hsu, Hsin Yi; Huang, Din Wen; Hsu, Hsing Hua; Lin, Ju Tsui; Shih, Chun Kuang; Chiang, Wenchang.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 13, 14.07.2010, p. 7616-7623.

Research output: Contribution to journalArticle

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abstract = "Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) is a grass crop and was reported to possess antiinflammatory activity and an antiproliferative effect in cancer cell lines. The purpose of this study was to evaluate the effects of the ethyl acetate fraction of an adlay bran ethanolic extract (ABE-Ea) on colon carcinogenesis in an animal model and investigate its mechanism. Male F344 rats received 1,2-dimethylhydrazine (DMH) and consumed different doses of ABE-Ea. The medium-dose group (17.28 mg of ABE-Ea/day) exhibited the best suppressive effect on colon carcinogenesis and prevented preneoplastic mucin-depleted foci (MDF) formation. Moreover, RAS and Ets2 oncogenes were significantly down-regulated in this group compared to the negative control group, whereas Wee 7, a gene involved in the cell cycle, was up-regulated. Cyclooxygenase-2 (COX-2) protein expression was significantly suppressed in all colons receiving the ABE-Ea, indicating that ABE-Ea delayed carcinogenesis by suppressing chronic inflammation. ABE-Ea included considerable a proportion of phenolic compounds, and ferulic acid was the major phenolic acid (5206 μg/g ABE-Ea) on the basis of HPLC analysis. Results from this study suggest that ABE-Ea suppressed DMH-indued preneoplastic lesions of the colon in F344 rats and that ferulic acid may be one of the active compounds.",
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AU - Chung, Cheng Pei

AU - Hsu, Hsin Yi

AU - Huang, Din Wen

AU - Hsu, Hsing Hua

AU - Lin, Ju Tsui

AU - Shih, Chun Kuang

AU - Chiang, Wenchang

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