Ethyl acetate fraction of adlay bran ethanolic extract inhibits oncogene expression and suppresses DMH-induced preneoplastic lesions of the colon in F344 rats through an anti-inflammatory pathway

Cheng Pei Chung, Hsin Yi Hsu, Din Wen Huang, Hsing Hua Hsu, Ju Tsui Lin, Chun Kuang Shih, Wenchang Chiang

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25 Citations (Scopus)


Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) is a grass crop and was reported to possess antiinflammatory activity and an antiproliferative effect in cancer cell lines. The purpose of this study was to evaluate the effects of the ethyl acetate fraction of an adlay bran ethanolic extract (ABE-Ea) on colon carcinogenesis in an animal model and investigate its mechanism. Male F344 rats received 1,2-dimethylhydrazine (DMH) and consumed different doses of ABE-Ea. The medium-dose group (17.28 mg of ABE-Ea/day) exhibited the best suppressive effect on colon carcinogenesis and prevented preneoplastic mucin-depleted foci (MDF) formation. Moreover, RAS and Ets2 oncogenes were significantly down-regulated in this group compared to the negative control group, whereas Wee 7, a gene involved in the cell cycle, was up-regulated. Cyclooxygenase-2 (COX-2) protein expression was significantly suppressed in all colons receiving the ABE-Ea, indicating that ABE-Ea delayed carcinogenesis by suppressing chronic inflammation. ABE-Ea included considerable a proportion of phenolic compounds, and ferulic acid was the major phenolic acid (5206 μg/g ABE-Ea) on the basis of HPLC analysis. Results from this study suggest that ABE-Ea suppressed DMH-indued preneoplastic lesions of the colon in F344 rats and that ferulic acid may be one of the active compounds.

Original languageEnglish
Pages (from-to)7616-7623
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Issue number13
Publication statusPublished - Jul 14 2010



  • Adlay bran
  • Cyclooxygenase-2 (cox-2)
  • Mucin-depleted foci (mdf)
  • RAS
  • Wee1

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Medicine(all)

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