Ethanol extracts of fruiting bodies of antrodia cinnamomea suppress CL1-5 human lung adenocarcinoma cells migration by inhibiting matrix metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt signaling pathways

Ying Yi Chen, Fon Chang Liu, Pei Yu Chou, Yi Chung Chien, Wun Shaing Wayne Chang, Guang Jhong Huang, Chieh Hsi Wu, Ming Jyh Sheu

Research output: Contribution to journalArticle

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Abstract

Cancer metastasis is a primary cause of cancer death. Antrodia cinnamomea (A. cinnamomea), a medicinal mushroom in Taiwan, has shown antioxidant and anticancer activities. In this study, we first observed that ethanol extract of fruiting bodies of A. cinnamomea (EEAC) exerted a concentration-dependent inhibitory effect on migration and motility of the highly metastatic CL1-5 cells in the absence of cytotoxicity. The results of a gelatin zymography assay showed that A. cinnamomea suppressed the activities of matrix metalloproteinase-(MMP-) 2 and MMP-9 in a concentration-dependent manner. Western blot results demonstrated that treatment with A. cinnamomea decreased the expression of MMP-9 and MMP-2; while the expression of the endogenous inhibitors of these proteins, that is, tissue inhibitors of MMP (TIMP-1 and TIMP-2) increased. Further investigation revealed that A. cinnamomea suppressed the phosphorylation of ERK1/2, p38, and JNK1/2. A. cinnamomea also suppressed the expressions of PI3K and phosphorylation of Akt. Furthermore, treatment of CL1-5 cells with inhibitors specific for PI3K (LY 294002), ERK1/2 (PD98059), JNK (SP600125), and p38 MAPK (SB203580) decreased the expression of MMP-2 and MMP-9. This is the first paper confirming the antimigration activity of this potentially beneficial mushroom against human lung adenocarcinoma CL1-5 cancer cells.

Original languageEnglish
Article number378415
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012
Externally publishedYes

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Antrodia
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Matrix Metalloproteinases
Phosphatidylinositol 3-Kinases
Cell Movement
Ethanol
Agaricales
Phosphorylation
Tissue Inhibitor of Metalloproteinase-2
Neoplasms
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
p38 Mitogen-Activated Protein Kinases
Gelatin
Taiwan
Adenocarcinoma of lung
Cause of Death
Antioxidants

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Ethanol extracts of fruiting bodies of antrodia cinnamomea suppress CL1-5 human lung adenocarcinoma cells migration by inhibiting matrix metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt signaling pathways. / Chen, Ying Yi; Liu, Fon Chang; Chou, Pei Yu; Chien, Yi Chung; Chang, Wun Shaing Wayne; Huang, Guang Jhong; Wu, Chieh Hsi; Sheu, Ming Jyh.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 378415, 2012.

Research output: Contribution to journalArticle

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abstract = "Cancer metastasis is a primary cause of cancer death. Antrodia cinnamomea (A. cinnamomea), a medicinal mushroom in Taiwan, has shown antioxidant and anticancer activities. In this study, we first observed that ethanol extract of fruiting bodies of A. cinnamomea (EEAC) exerted a concentration-dependent inhibitory effect on migration and motility of the highly metastatic CL1-5 cells in the absence of cytotoxicity. The results of a gelatin zymography assay showed that A. cinnamomea suppressed the activities of matrix metalloproteinase-(MMP-) 2 and MMP-9 in a concentration-dependent manner. Western blot results demonstrated that treatment with A. cinnamomea decreased the expression of MMP-9 and MMP-2; while the expression of the endogenous inhibitors of these proteins, that is, tissue inhibitors of MMP (TIMP-1 and TIMP-2) increased. Further investigation revealed that A. cinnamomea suppressed the phosphorylation of ERK1/2, p38, and JNK1/2. A. cinnamomea also suppressed the expressions of PI3K and phosphorylation of Akt. Furthermore, treatment of CL1-5 cells with inhibitors specific for PI3K (LY 294002), ERK1/2 (PD98059), JNK (SP600125), and p38 MAPK (SB203580) decreased the expression of MMP-2 and MMP-9. This is the first paper confirming the antimigration activity of this potentially beneficial mushroom against human lung adenocarcinoma CL1-5 cancer cells.",
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AU - Chen, Ying Yi

AU - Liu, Fon Chang

AU - Chou, Pei Yu

AU - Chien, Yi Chung

AU - Chang, Wun Shaing Wayne

AU - Huang, Guang Jhong

AU - Wu, Chieh Hsi

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