Estrogen modulates the spinal N-methyl-D-aspartic acid-mediated pelvic nerve-to-urethra reflex plasticity in rats

Sheng Yen Lin, Gin Den Chen, Jiuan Miaw Liao, Shwu Fen Pan, Mei Jung Chen, Joyce C. Chen, Hsien Yu Peng, Yu Cheng Ho, Yen Chun Ho, Pei Chen Huang, Jau Jia Lin, Tzer Bin Lin

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Abstract

The effects of gonadal steroids on glutamate-mediated pelvic nerve-to-urethra reflex (PUR) plasticity were investigated in rats, which received a sham operation (Sham), ovariectomy (OVX), or ovariectomy with daily supplemental estrogen (50 μg/kg, OVX ± E2). The magnitude of the repetitive stimulation (RS, 1 Hz)-induced potentiation in PUR activity decreased significantly in the OVX group when compared with the Sham groups (18.09 ± 3.91 and 7.40 ± 1.03 spikes/stimulation in Sham and OVX group; respectively, P <0.01, n = 21). Supplemental estrogen (OVX + E 2, 12.60 ± 1.49 spikes/stimulation) significantly reversed the decrease in RS-induced PUR potentiation caused by OVX (P <0.01, n = 21). The magnitude of the RS-induced potentiation in PUR activity decreased significantly after intrathecal 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo (F) quinoxaline [(20 μM, 10 μl), from 18.09 ± 3.91 to 10.40 ± 0.81, from 7.40 ± 1.03 to 3.20 ± 0.94, and from 12.60 ± 1.49 to 8.06 ± 0.32 spikes/stimulation in Sham, OVX, and OVX + E 2, respectively, P <0.01, n = 18] and D-2-amino-5- phosphonoraleric acid [(100 μM, 10 μl), from 18.09 ± 3.91 to 1.04 ± 0.12, from 7.40 ± 1.03 to 1.06 ± 0.22, and from 12.60 ± 1.49 to 0.98 ± 0.25 spikes/stimulation in Sham, OVX, and OVX + E2, respectively, P <0.01, n = 18]. In addition, potentiation in PUR activities was induced by intrathecal L-glutamate (0.1 mM, 10 μl, from 1.04 ± 0.02 to 21.60 ± 0.93, from 1.10 ± 0.06 to 8.40 ± 1.50, and from 1.03 ± 0.03 to 18.04 ± 0.84 spikes/stimulation in Sham, OVX, and OVX + E2, respectively, P <0.01, n = 18) and N-methyl-D-aspartic acid (0.1 mM, 10 μl, from 1.04 ± 0.02 to 14.80 ± 0.97, from 1.10 ± 0.06 to 4.60 ± 0.48, and from 1.03 ± 0.03 to 9.09 ± 0.63 spikes/stimulation in Sham, OVX, and OVX + E2); N-methyl-D-aspartic acid-mediated PUR plasticity in female rats and may contribute to alterations in urinary dysfunction after menopause.

Original languageEnglish
Pages (from-to)2956-2963
Number of pages8
JournalEndocrinology
Volume147
Issue number6
DOIs
Publication statusPublished - Jun 2006
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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    Lin, S. Y., Chen, G. D., Liao, J. M., Pan, S. F., Chen, M. J., Chen, J. C., Peng, H. Y., Ho, Y. C., Ho, Y. C., Huang, P. C., Lin, J. J., & Lin, T. B. (2006). Estrogen modulates the spinal N-methyl-D-aspartic acid-mediated pelvic nerve-to-urethra reflex plasticity in rats. Endocrinology, 147(6), 2956-2963. https://doi.org/10.1210/en.2005-1378