Establishment of Human Mesothelioma Cell Lines (MS-1, -2) and Production of a Monoclonal Antibody (Anti-MS) with Diagnostic and Therapeutic Potential

Su Ming Hsu, Pei Line Hsu, Xun Zhao, Chien Song Kao-Shan, Jacqueline Whang-Peng

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have established and characterized two mesothelioma cell lines, MS-1 and MS-2, in four attempts at long-term culture of these cells. Both MS-1 and MS-2 cells consistently express cytokeratin and vimentin, and both have long, slender microvilli. The cells that grew indefinitely (MS-1, MS-2) had a higher DNA index and a higher nucleus:cytoplasm ratio than did those cells that failed to grow (MS-3, MS-4). All mesothelioma cells, both in short-and in long-term culture, responded to phorbol ester induction by displaying morphological differentiation, such as an increase in the number of microvilli. The distribution of vimentin and cytokeratin in the cells, however, remained the same regardless of the growth pattern or the phorbol-ester treatment of the cells. We have used MS-1 cells to produce a monoclonal antibody (anti-MS) that reacts with mesothelioma cells, but rarely with reactive or normal mesothelial cells or with cells of normal tissues. The antibody does not induce the modulation of antigen, and it causes no direct or complement-mediated cytotoxicity of MS-1 or MS-2 cells. If a toxin or an isotope conjugate of the antibody is used, it may be valuable in the near future to test it for use in immunoimaging or immunotherapy.

Original languageEnglish
Pages (from-to)5228-5236
Number of pages9
JournalCancer Research
Volume48
Issue number18
Publication statusPublished - Jan 1 1988
Externally publishedYes

Fingerprint

Mesothelioma
Monoclonal Antibodies
Cell Line
Therapeutics
Phorbol Esters
Vimentin
Microvilli
Keratins
MS 1-2
Antibodies
Isotopes
Immunotherapy
Cytoplasm
Cell Culture Techniques
Antigens

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Establishment of Human Mesothelioma Cell Lines (MS-1, -2) and Production of a Monoclonal Antibody (Anti-MS) with Diagnostic and Therapeutic Potential. / Hsu, Su Ming; Hsu, Pei Line; Zhao, Xun; Kao-Shan, Chien Song; Whang-Peng, Jacqueline.

In: Cancer Research, Vol. 48, No. 18, 01.01.1988, p. 5228-5236.

Research output: Contribution to journalArticle

@article{06d1f18944004bf2b385c609e8c77ee4,
title = "Establishment of Human Mesothelioma Cell Lines (MS-1, -2) and Production of a Monoclonal Antibody (Anti-MS) with Diagnostic and Therapeutic Potential",
abstract = "We have established and characterized two mesothelioma cell lines, MS-1 and MS-2, in four attempts at long-term culture of these cells. Both MS-1 and MS-2 cells consistently express cytokeratin and vimentin, and both have long, slender microvilli. The cells that grew indefinitely (MS-1, MS-2) had a higher DNA index and a higher nucleus:cytoplasm ratio than did those cells that failed to grow (MS-3, MS-4). All mesothelioma cells, both in short-and in long-term culture, responded to phorbol ester induction by displaying morphological differentiation, such as an increase in the number of microvilli. The distribution of vimentin and cytokeratin in the cells, however, remained the same regardless of the growth pattern or the phorbol-ester treatment of the cells. We have used MS-1 cells to produce a monoclonal antibody (anti-MS) that reacts with mesothelioma cells, but rarely with reactive or normal mesothelial cells or with cells of normal tissues. The antibody does not induce the modulation of antigen, and it causes no direct or complement-mediated cytotoxicity of MS-1 or MS-2 cells. If a toxin or an isotope conjugate of the antibody is used, it may be valuable in the near future to test it for use in immunoimaging or immunotherapy.",
author = "Hsu, {Su Ming} and Hsu, {Pei Line} and Xun Zhao and Kao-Shan, {Chien Song} and Jacqueline Whang-Peng",
year = "1988",
month = "1",
day = "1",
language = "English",
volume = "48",
pages = "5228--5236",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "18",

}

TY - JOUR

T1 - Establishment of Human Mesothelioma Cell Lines (MS-1, -2) and Production of a Monoclonal Antibody (Anti-MS) with Diagnostic and Therapeutic Potential

AU - Hsu, Su Ming

AU - Hsu, Pei Line

AU - Zhao, Xun

AU - Kao-Shan, Chien Song

AU - Whang-Peng, Jacqueline

PY - 1988/1/1

Y1 - 1988/1/1

N2 - We have established and characterized two mesothelioma cell lines, MS-1 and MS-2, in four attempts at long-term culture of these cells. Both MS-1 and MS-2 cells consistently express cytokeratin and vimentin, and both have long, slender microvilli. The cells that grew indefinitely (MS-1, MS-2) had a higher DNA index and a higher nucleus:cytoplasm ratio than did those cells that failed to grow (MS-3, MS-4). All mesothelioma cells, both in short-and in long-term culture, responded to phorbol ester induction by displaying morphological differentiation, such as an increase in the number of microvilli. The distribution of vimentin and cytokeratin in the cells, however, remained the same regardless of the growth pattern or the phorbol-ester treatment of the cells. We have used MS-1 cells to produce a monoclonal antibody (anti-MS) that reacts with mesothelioma cells, but rarely with reactive or normal mesothelial cells or with cells of normal tissues. The antibody does not induce the modulation of antigen, and it causes no direct or complement-mediated cytotoxicity of MS-1 or MS-2 cells. If a toxin or an isotope conjugate of the antibody is used, it may be valuable in the near future to test it for use in immunoimaging or immunotherapy.

AB - We have established and characterized two mesothelioma cell lines, MS-1 and MS-2, in four attempts at long-term culture of these cells. Both MS-1 and MS-2 cells consistently express cytokeratin and vimentin, and both have long, slender microvilli. The cells that grew indefinitely (MS-1, MS-2) had a higher DNA index and a higher nucleus:cytoplasm ratio than did those cells that failed to grow (MS-3, MS-4). All mesothelioma cells, both in short-and in long-term culture, responded to phorbol ester induction by displaying morphological differentiation, such as an increase in the number of microvilli. The distribution of vimentin and cytokeratin in the cells, however, remained the same regardless of the growth pattern or the phorbol-ester treatment of the cells. We have used MS-1 cells to produce a monoclonal antibody (anti-MS) that reacts with mesothelioma cells, but rarely with reactive or normal mesothelial cells or with cells of normal tissues. The antibody does not induce the modulation of antigen, and it causes no direct or complement-mediated cytotoxicity of MS-1 or MS-2 cells. If a toxin or an isotope conjugate of the antibody is used, it may be valuable in the near future to test it for use in immunoimaging or immunotherapy.

UR - http://www.scopus.com/inward/record.url?scp=0023742563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023742563&partnerID=8YFLogxK

M3 - Article

C2 - 2457437

AN - SCOPUS:0023742563

VL - 48

SP - 5228

EP - 5236

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 18

ER -