Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia

Yi Wei Huang, Rouh Mei Hu, Yu Ting Chiang, Tsao Chuen Chung, Tung Ching Chung, Tsuey Ching Yang

Research output: Contribution to journalArticle

Abstract

A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1% to 0.5% for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia.

Original languageEnglish
Pages (from-to)18-22
Number of pages5
JournalFolia Microbiologica
Volume56
Issue number1
DOIs
Publication statusPublished - Jan 2011

ASJC Scopus subject areas

  • Microbiology

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    Huang, Y. W., Hu, R. M., Chiang, Y. T., Chung, T. C., Chung, T. C., & Yang, T. C. (2011). Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia. Folia Microbiologica, 56(1), 18-22. https://doi.org/10.1007/s12223-011-0008-2