Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia

Yi Wei Huang, Rouh Mei Hu, Yu Ting Chiang, Tsao Chuen Chung, Tung Ching Chung, Tsuey Ching Yang

Research output: Contribution to journalArticle

Abstract

A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1% to 0.5% for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia.

Original languageEnglish
Pages (from-to)18-22
Number of pages5
JournalFolia Microbiologica
Volume56
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Stenotrophomonas maltophilia
Arabinose
Catechol 2,3-Dioxygenase
Gene Fusion
Cross Infection
Carbon
Genes

ASJC Scopus subject areas

  • Microbiology

Cite this

Huang, Y. W., Hu, R. M., Chiang, Y. T., Chung, T. C., Chung, T. C., & Yang, T. C. (2011). Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia. Folia Microbiologica, 56(1), 18-22. https://doi.org/10.1007/s12223-011-0008-2

Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia. / Huang, Yi Wei; Hu, Rouh Mei; Chiang, Yu Ting; Chung, Tsao Chuen; Chung, Tung Ching; Yang, Tsuey Ching.

In: Folia Microbiologica, Vol. 56, No. 1, 01.2011, p. 18-22.

Research output: Contribution to journalArticle

Huang, YW, Hu, RM, Chiang, YT, Chung, TC, Chung, TC & Yang, TC 2011, 'Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia', Folia Microbiologica, vol. 56, no. 1, pp. 18-22. https://doi.org/10.1007/s12223-011-0008-2
Huang, Yi Wei ; Hu, Rouh Mei ; Chiang, Yu Ting ; Chung, Tsao Chuen ; Chung, Tung Ching ; Yang, Tsuey Ching. / Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia. In: Folia Microbiologica. 2011 ; Vol. 56, No. 1. pp. 18-22.
@article{7493643950d94e0aba15bc60df004eb2,
title = "Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia",
abstract = "A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1{\%} to 0.5{\%} for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia.",
author = "Huang, {Yi Wei} and Hu, {Rouh Mei} and Chiang, {Yu Ting} and Chung, {Tsao Chuen} and Chung, {Tung Ching} and Yang, {Tsuey Ching}",
year = "2011",
month = "1",
doi = "10.1007/s12223-011-0008-2",
language = "English",
volume = "56",
pages = "18--22",
journal = "Folia Microbiologica",
issn = "0015-5632",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia

AU - Huang, Yi Wei

AU - Hu, Rouh Mei

AU - Chiang, Yu Ting

AU - Chung, Tsao Chuen

AU - Chung, Tung Ching

AU - Yang, Tsuey Ching

PY - 2011/1

Y1 - 2011/1

N2 - A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1% to 0.5% for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia.

AB - A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1% to 0.5% for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia.

UR - http://www.scopus.com/inward/record.url?scp=79955840166&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955840166&partnerID=8YFLogxK

U2 - 10.1007/s12223-011-0008-2

DO - 10.1007/s12223-011-0008-2

M3 - Article

C2 - 21503736

VL - 56

SP - 18

EP - 22

JO - Folia Microbiologica

JF - Folia Microbiologica

SN - 0015-5632

IS - 1

ER -