Establishment and characterization of a malignant glioma cell line, GBM8401/TSGH,NDMC

W. H. Lee; M. Y. Yeh; Y. C. Tu; S. H. Han; Y. C. Wang

Establishment and characterization of a malignant glioma cell line, GBM8401/TSGH,NDMC

W. H. Lee, M. Y. Yeh, Y. C. Tu, S. H. Han, Y. C. Wang

Research output: Contribution to journal › Article › peer-review

Abstract

A permanent human brain malignant glioma cell line, GBM8401/TSGH,NDMC, has been successfully established from a 31-year-old Chinese female with brain glioblastoma multiforme (GBM) in monolayer culture and has been subcultured for more than 100 passages during 24 months in vitro. The tumor cell doubling time in vitro was approximately 38 hr. The tumorigenicity in athymic nude mice was observed; the tumor volume doubling time was approximately 4 days. Glial fibrillary acidic protein (GFAP) and 10-nm-diameter intermediate filaments were identified by immunohistochemical peroxidase-antiperoxidase (PAP), immunofluorescence assay, and transmission electron microscopic methods. The scanning electron microscope revealed numerous surface microvilli and various-sized blebs. Karyotypic analysis showed this malignant glioma cell line to be of human origin, near-diploid with a modal chromosome number of 48,XX.

Original language	English
Pages (from-to)	173-181
Number of pages	9
Journal	Journal of Surgical Oncology
Volume	38
Issue number	3
Publication status	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

Oncology
Surgery

Cite this

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title = "Establishment and characterization of a malignant glioma cell line, GBM8401/TSGH,NDMC",

abstract = "A permanent human brain malignant glioma cell line, GBM8401/TSGH,NDMC, has been successfully established from a 31-year-old Chinese female with brain glioblastoma multiforme (GBM) in monolayer culture and has been subcultured for more than 100 passages during 24 months in vitro. The tumor cell doubling time in vitro was approximately 38 hr. The tumorigenicity in athymic nude mice was observed; the tumor volume doubling time was approximately 4 days. Glial fibrillary acidic protein (GFAP) and 10-nm-diameter intermediate filaments were identified by immunohistochemical peroxidase-antiperoxidase (PAP), immunofluorescence assay, and transmission electron microscopic methods. The scanning electron microscope revealed numerous surface microvilli and various-sized blebs. Karyotypic analysis showed this malignant glioma cell line to be of human origin, near-diploid with a modal chromosome number of 48,XX.",

author = "Lee, {W. H.} and Yeh, {M. Y.} and Tu, {Y. C.} and Han, {S. H.} and Wang, {Y. C.}",

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T1 - Establishment and characterization of a malignant glioma cell line, GBM8401/TSGH,NDMC

AU - Lee, W. H.

AU - Yeh, M. Y.

AU - Tu, Y. C.

AU - Han, S. H.

AU - Wang, Y. C.

PY - 1988

Y1 - 1988

N2 - A permanent human brain malignant glioma cell line, GBM8401/TSGH,NDMC, has been successfully established from a 31-year-old Chinese female with brain glioblastoma multiforme (GBM) in monolayer culture and has been subcultured for more than 100 passages during 24 months in vitro. The tumor cell doubling time in vitro was approximately 38 hr. The tumorigenicity in athymic nude mice was observed; the tumor volume doubling time was approximately 4 days. Glial fibrillary acidic protein (GFAP) and 10-nm-diameter intermediate filaments were identified by immunohistochemical peroxidase-antiperoxidase (PAP), immunofluorescence assay, and transmission electron microscopic methods. The scanning electron microscope revealed numerous surface microvilli and various-sized blebs. Karyotypic analysis showed this malignant glioma cell line to be of human origin, near-diploid with a modal chromosome number of 48,XX.

AB - A permanent human brain malignant glioma cell line, GBM8401/TSGH,NDMC, has been successfully established from a 31-year-old Chinese female with brain glioblastoma multiforme (GBM) in monolayer culture and has been subcultured for more than 100 passages during 24 months in vitro. The tumor cell doubling time in vitro was approximately 38 hr. The tumorigenicity in athymic nude mice was observed; the tumor volume doubling time was approximately 4 days. Glial fibrillary acidic protein (GFAP) and 10-nm-diameter intermediate filaments were identified by immunohistochemical peroxidase-antiperoxidase (PAP), immunofluorescence assay, and transmission electron microscopic methods. The scanning electron microscope revealed numerous surface microvilli and various-sized blebs. Karyotypic analysis showed this malignant glioma cell line to be of human origin, near-diploid with a modal chromosome number of 48,XX.

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Establishment and characterization of a malignant glioma cell line, GBM8401/TSGH,NDMC

Abstract

ASJC Scopus subject areas

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