Escherichia coli capsular polysaccharide synthesis, antibiotic susceptibility, and red blood cell agglutination

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Abstract

Background: Escherichia coli is a normal component of the human intestinal flora, but it is also a pathogen particularly in women with urinary tract infections, cystitis, and kidney diseases. A new capsular polysaccharide (CPS)-synthesizing variant strain of E.coli was isolated from a culturing bacterial medium containing proteose peptone No. 3 glycerin salt in our previous study. In this study, we further isolated a new E.coli variant, which produces capsular polysaccharides (CEF-CPS), using cefazolin (CEF) and examined the pathogenic characteristics of this CEF-CPS strain. Methods: Polysaccharides produced by CEF-treated E.coli were separated and purified using anion exchange resin and gel filtration column chromatographic methods. The red blood cell (RBC) agglutination assay and antibiotic susceptibility test were conducted, and serum bactericidal activity, antibiotic tolerance, and antibiotic uptake were also examined. Results: (1) Variant strains had greater RBC hemagglutination ability than parental strains. (2) The CEF-CPS variant strain showed a two-fold increase compared to the parental strain. (3) Antibiotic resistance to CEF, ampicillin, and polymyxin B was increased four-fold in the CEF-CPS variant strain. (4) Bacterial cell counts in the parental strain incubated in a medium containing gentamicin were reduced significantly, near to the undetectable level, within 4 hours. By contrast, bacterial counts of the CEF-CPS strain displayed only 50% reduction compared to the original bacterial counts under the same culture environment. Conclusion: E.coli derived from normal human intestinal flora generated a new variant strain along with the production of newly synthesized additional polysaccharides on the cell wall in the presence of CEF. Their productions can contribute to enhancing multiple-drug resistance and pathogenicity.

Original languageEnglish
Pages (from-to)16-20
Number of pages5
JournalJournal of Experimental and Clinical Medicine(Taiwan)
Volume6
Issue number1
DOIs
Publication statusPublished - Feb 2014

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Cefazolin
Agglutination
Polysaccharides
Erythrocytes
Escherichia coli
Anti-Bacterial Agents
Bacterial Load
Anion Exchange Resins
Polymyxin B
Cystitis
Hemagglutination
Kidney Diseases
Multiple Drug Resistance
Ampicillin
Microbial Drug Resistance
Gentamicins
Urinary Tract Infections
Glycerol
Cell Wall
Gel Chromatography

Keywords

  • Antibiotic resistance
  • Human serum bactericidal activity
  • Red blood cell hemagglutination

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Escherichia coli capsular polysaccharide synthesis, antibiotic susceptibility, and red blood cell agglutination",
abstract = "Background: Escherichia coli is a normal component of the human intestinal flora, but it is also a pathogen particularly in women with urinary tract infections, cystitis, and kidney diseases. A new capsular polysaccharide (CPS)-synthesizing variant strain of E.coli was isolated from a culturing bacterial medium containing proteose peptone No. 3 glycerin salt in our previous study. In this study, we further isolated a new E.coli variant, which produces capsular polysaccharides (CEF-CPS), using cefazolin (CEF) and examined the pathogenic characteristics of this CEF-CPS strain. Methods: Polysaccharides produced by CEF-treated E.coli were separated and purified using anion exchange resin and gel filtration column chromatographic methods. The red blood cell (RBC) agglutination assay and antibiotic susceptibility test were conducted, and serum bactericidal activity, antibiotic tolerance, and antibiotic uptake were also examined. Results: (1) Variant strains had greater RBC hemagglutination ability than parental strains. (2) The CEF-CPS variant strain showed a two-fold increase compared to the parental strain. (3) Antibiotic resistance to CEF, ampicillin, and polymyxin B was increased four-fold in the CEF-CPS variant strain. (4) Bacterial cell counts in the parental strain incubated in a medium containing gentamicin were reduced significantly, near to the undetectable level, within 4 hours. By contrast, bacterial counts of the CEF-CPS strain displayed only 50{\%} reduction compared to the original bacterial counts under the same culture environment. Conclusion: E.coli derived from normal human intestinal flora generated a new variant strain along with the production of newly synthesized additional polysaccharides on the cell wall in the presence of CEF. Their productions can contribute to enhancing multiple-drug resistance and pathogenicity.",
keywords = "Antibiotic resistance, Human serum bactericidal activity, Red blood cell hemagglutination",
author = "Chin, {Yi Ping} and Chang, {Shwu Fen} and Tseng, {Cheng Chuang} and Chen, {Mei Chieh}",
year = "2014",
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T1 - Escherichia coli capsular polysaccharide synthesis, antibiotic susceptibility, and red blood cell agglutination

AU - Chin, Yi Ping

AU - Chang, Shwu Fen

AU - Tseng, Cheng Chuang

AU - Chen, Mei Chieh

PY - 2014/2

Y1 - 2014/2

N2 - Background: Escherichia coli is a normal component of the human intestinal flora, but it is also a pathogen particularly in women with urinary tract infections, cystitis, and kidney diseases. A new capsular polysaccharide (CPS)-synthesizing variant strain of E.coli was isolated from a culturing bacterial medium containing proteose peptone No. 3 glycerin salt in our previous study. In this study, we further isolated a new E.coli variant, which produces capsular polysaccharides (CEF-CPS), using cefazolin (CEF) and examined the pathogenic characteristics of this CEF-CPS strain. Methods: Polysaccharides produced by CEF-treated E.coli were separated and purified using anion exchange resin and gel filtration column chromatographic methods. The red blood cell (RBC) agglutination assay and antibiotic susceptibility test were conducted, and serum bactericidal activity, antibiotic tolerance, and antibiotic uptake were also examined. Results: (1) Variant strains had greater RBC hemagglutination ability than parental strains. (2) The CEF-CPS variant strain showed a two-fold increase compared to the parental strain. (3) Antibiotic resistance to CEF, ampicillin, and polymyxin B was increased four-fold in the CEF-CPS variant strain. (4) Bacterial cell counts in the parental strain incubated in a medium containing gentamicin were reduced significantly, near to the undetectable level, within 4 hours. By contrast, bacterial counts of the CEF-CPS strain displayed only 50% reduction compared to the original bacterial counts under the same culture environment. Conclusion: E.coli derived from normal human intestinal flora generated a new variant strain along with the production of newly synthesized additional polysaccharides on the cell wall in the presence of CEF. Their productions can contribute to enhancing multiple-drug resistance and pathogenicity.

AB - Background: Escherichia coli is a normal component of the human intestinal flora, but it is also a pathogen particularly in women with urinary tract infections, cystitis, and kidney diseases. A new capsular polysaccharide (CPS)-synthesizing variant strain of E.coli was isolated from a culturing bacterial medium containing proteose peptone No. 3 glycerin salt in our previous study. In this study, we further isolated a new E.coli variant, which produces capsular polysaccharides (CEF-CPS), using cefazolin (CEF) and examined the pathogenic characteristics of this CEF-CPS strain. Methods: Polysaccharides produced by CEF-treated E.coli were separated and purified using anion exchange resin and gel filtration column chromatographic methods. The red blood cell (RBC) agglutination assay and antibiotic susceptibility test were conducted, and serum bactericidal activity, antibiotic tolerance, and antibiotic uptake were also examined. Results: (1) Variant strains had greater RBC hemagglutination ability than parental strains. (2) The CEF-CPS variant strain showed a two-fold increase compared to the parental strain. (3) Antibiotic resistance to CEF, ampicillin, and polymyxin B was increased four-fold in the CEF-CPS variant strain. (4) Bacterial cell counts in the parental strain incubated in a medium containing gentamicin were reduced significantly, near to the undetectable level, within 4 hours. By contrast, bacterial counts of the CEF-CPS strain displayed only 50% reduction compared to the original bacterial counts under the same culture environment. Conclusion: E.coli derived from normal human intestinal flora generated a new variant strain along with the production of newly synthesized additional polysaccharides on the cell wall in the presence of CEF. Their productions can contribute to enhancing multiple-drug resistance and pathogenicity.

KW - Antibiotic resistance

KW - Human serum bactericidal activity

KW - Red blood cell hemagglutination

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