Epstein-Barr virus-associated gastric adenocarcinoma in Taiwan

Horng Jyh Harn, Jang Yang Chang, Min Wei Wang, Li Ing Ho, Herng Sheng Lee, Jung Hwa Chiang, Wei Hwa Lee

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Fifty-five gastric carcinoma tumors from Chinese patients in Taiwan, including 40 tubular type (one lymphoepthelioma-like carcinoma subtype), eight signet ring cell type, one papillary type, and six mucinous type gastric carcinomas, were investigated for the presence of Epstein-Barr virus (EBV) transcripts by in situ hybridization using fluorescein-conjugated EBV oligonucleotides for EBERs (Epstein-Barr virus early RNAs) expression and the polymerase chain reaction for viral DNA. Epstein-Barr virus was detected in six of 55 lesions (11%), a significantly lower proportion than has been observed in a North American series. Epstein-Barr virus involvement was more common among male patients. Epstein-Barr virus DNA and its EBERs were specifically present within gastric carcinoma and adjacent dysplastic cells but were absent in surrounding lymphocytes and normal gastric mucosa. Epstein-Barr virus DNA and EBERs were found in one sample of lymphoepithelioma-like carcinoma (LELC) and five specimens of typical gastric adenocarcinoma. Among the EBV-positive gastric adenocarcinomas, four were tubular type of varied differentiation and one was signet ring cell type. Furthermore, we evaluated the expression of the latent membrane protein (LMP) with monoclonal antibodies. We found that LMP was expressed in two EBVpositive samples. In addition, the presence of the EBV receptor was studied by probing samples with CD21 monoclonal antibody. Epstein-Barr virus receptor was not detected in any sample. Southern blot analysis indicated single clonal proliferation of tumor cells. These findings confirm and extend the results of Shibata et al. They also indicate that EBV infection might be related to oncogenesis not only in rare gastric cancers that resemble nasopharyngeal lymphoepithelioma but also in typical gastric adenocarcinoma.

Original languageEnglish
Pages (from-to)267-271
Number of pages5
JournalHuman Pathology
Volume26
Issue number3
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Human Herpesvirus 4
Taiwan
Stomach
Adenocarcinoma
Complement 3d Receptors
Carcinoma
Membrane Proteins
Monoclonal Antibodies
RNA
Mucinous Adenocarcinoma
Epstein-Barr Virus Infections
DNA
Viral DNA
DNA-Directed RNA Polymerases
Southern Blotting
Gastric Mucosa
Fluorescein
Oligonucleotides
Stomach Neoplasms
In Situ Hybridization

Keywords

  • Epstein-Barr virus
  • gastric adenocarcinoma
  • in situ hybridization
  • polymerase chain reaction
  • Southern blot analysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Harn, H. J., Chang, J. Y., Wang, M. W., Ho, L. I., Lee, H. S., Chiang, J. H., & Lee, W. H. (1995). Epstein-Barr virus-associated gastric adenocarcinoma in Taiwan. Human Pathology, 26(3), 267-271. https://doi.org/10.1016/0046-8177(95)90056-X

Epstein-Barr virus-associated gastric adenocarcinoma in Taiwan. / Harn, Horng Jyh; Chang, Jang Yang; Wang, Min Wei; Ho, Li Ing; Lee, Herng Sheng; Chiang, Jung Hwa; Lee, Wei Hwa.

In: Human Pathology, Vol. 26, No. 3, 1995, p. 267-271.

Research output: Contribution to journalArticle

Harn, HJ, Chang, JY, Wang, MW, Ho, LI, Lee, HS, Chiang, JH & Lee, WH 1995, 'Epstein-Barr virus-associated gastric adenocarcinoma in Taiwan', Human Pathology, vol. 26, no. 3, pp. 267-271. https://doi.org/10.1016/0046-8177(95)90056-X
Harn HJ, Chang JY, Wang MW, Ho LI, Lee HS, Chiang JH et al. Epstein-Barr virus-associated gastric adenocarcinoma in Taiwan. Human Pathology. 1995;26(3):267-271. https://doi.org/10.1016/0046-8177(95)90056-X
Harn, Horng Jyh ; Chang, Jang Yang ; Wang, Min Wei ; Ho, Li Ing ; Lee, Herng Sheng ; Chiang, Jung Hwa ; Lee, Wei Hwa. / Epstein-Barr virus-associated gastric adenocarcinoma in Taiwan. In: Human Pathology. 1995 ; Vol. 26, No. 3. pp. 267-271.
@article{eb093c834a6f428687befed2f3d063d8,
title = "Epstein-Barr virus-associated gastric adenocarcinoma in Taiwan",
abstract = "Fifty-five gastric carcinoma tumors from Chinese patients in Taiwan, including 40 tubular type (one lymphoepthelioma-like carcinoma subtype), eight signet ring cell type, one papillary type, and six mucinous type gastric carcinomas, were investigated for the presence of Epstein-Barr virus (EBV) transcripts by in situ hybridization using fluorescein-conjugated EBV oligonucleotides for EBERs (Epstein-Barr virus early RNAs) expression and the polymerase chain reaction for viral DNA. Epstein-Barr virus was detected in six of 55 lesions (11{\%}), a significantly lower proportion than has been observed in a North American series. Epstein-Barr virus involvement was more common among male patients. Epstein-Barr virus DNA and its EBERs were specifically present within gastric carcinoma and adjacent dysplastic cells but were absent in surrounding lymphocytes and normal gastric mucosa. Epstein-Barr virus DNA and EBERs were found in one sample of lymphoepithelioma-like carcinoma (LELC) and five specimens of typical gastric adenocarcinoma. Among the EBV-positive gastric adenocarcinomas, four were tubular type of varied differentiation and one was signet ring cell type. Furthermore, we evaluated the expression of the latent membrane protein (LMP) with monoclonal antibodies. We found that LMP was expressed in two EBVpositive samples. In addition, the presence of the EBV receptor was studied by probing samples with CD21 monoclonal antibody. Epstein-Barr virus receptor was not detected in any sample. Southern blot analysis indicated single clonal proliferation of tumor cells. These findings confirm and extend the results of Shibata et al. They also indicate that EBV infection might be related to oncogenesis not only in rare gastric cancers that resemble nasopharyngeal lymphoepithelioma but also in typical gastric adenocarcinoma.",
keywords = "Epstein-Barr virus, gastric adenocarcinoma, in situ hybridization, polymerase chain reaction, Southern blot analysis",
author = "Harn, {Horng Jyh} and Chang, {Jang Yang} and Wang, {Min Wei} and Ho, {Li Ing} and Lee, {Herng Sheng} and Chiang, {Jung Hwa} and Lee, {Wei Hwa}",
year = "1995",
doi = "10.1016/0046-8177(95)90056-X",
language = "English",
volume = "26",
pages = "267--271",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders",
number = "3",

}

TY - JOUR

T1 - Epstein-Barr virus-associated gastric adenocarcinoma in Taiwan

AU - Harn, Horng Jyh

AU - Chang, Jang Yang

AU - Wang, Min Wei

AU - Ho, Li Ing

AU - Lee, Herng Sheng

AU - Chiang, Jung Hwa

AU - Lee, Wei Hwa

PY - 1995

Y1 - 1995

N2 - Fifty-five gastric carcinoma tumors from Chinese patients in Taiwan, including 40 tubular type (one lymphoepthelioma-like carcinoma subtype), eight signet ring cell type, one papillary type, and six mucinous type gastric carcinomas, were investigated for the presence of Epstein-Barr virus (EBV) transcripts by in situ hybridization using fluorescein-conjugated EBV oligonucleotides for EBERs (Epstein-Barr virus early RNAs) expression and the polymerase chain reaction for viral DNA. Epstein-Barr virus was detected in six of 55 lesions (11%), a significantly lower proportion than has been observed in a North American series. Epstein-Barr virus involvement was more common among male patients. Epstein-Barr virus DNA and its EBERs were specifically present within gastric carcinoma and adjacent dysplastic cells but were absent in surrounding lymphocytes and normal gastric mucosa. Epstein-Barr virus DNA and EBERs were found in one sample of lymphoepithelioma-like carcinoma (LELC) and five specimens of typical gastric adenocarcinoma. Among the EBV-positive gastric adenocarcinomas, four were tubular type of varied differentiation and one was signet ring cell type. Furthermore, we evaluated the expression of the latent membrane protein (LMP) with monoclonal antibodies. We found that LMP was expressed in two EBVpositive samples. In addition, the presence of the EBV receptor was studied by probing samples with CD21 monoclonal antibody. Epstein-Barr virus receptor was not detected in any sample. Southern blot analysis indicated single clonal proliferation of tumor cells. These findings confirm and extend the results of Shibata et al. They also indicate that EBV infection might be related to oncogenesis not only in rare gastric cancers that resemble nasopharyngeal lymphoepithelioma but also in typical gastric adenocarcinoma.

AB - Fifty-five gastric carcinoma tumors from Chinese patients in Taiwan, including 40 tubular type (one lymphoepthelioma-like carcinoma subtype), eight signet ring cell type, one papillary type, and six mucinous type gastric carcinomas, were investigated for the presence of Epstein-Barr virus (EBV) transcripts by in situ hybridization using fluorescein-conjugated EBV oligonucleotides for EBERs (Epstein-Barr virus early RNAs) expression and the polymerase chain reaction for viral DNA. Epstein-Barr virus was detected in six of 55 lesions (11%), a significantly lower proportion than has been observed in a North American series. Epstein-Barr virus involvement was more common among male patients. Epstein-Barr virus DNA and its EBERs were specifically present within gastric carcinoma and adjacent dysplastic cells but were absent in surrounding lymphocytes and normal gastric mucosa. Epstein-Barr virus DNA and EBERs were found in one sample of lymphoepithelioma-like carcinoma (LELC) and five specimens of typical gastric adenocarcinoma. Among the EBV-positive gastric adenocarcinomas, four were tubular type of varied differentiation and one was signet ring cell type. Furthermore, we evaluated the expression of the latent membrane protein (LMP) with monoclonal antibodies. We found that LMP was expressed in two EBVpositive samples. In addition, the presence of the EBV receptor was studied by probing samples with CD21 monoclonal antibody. Epstein-Barr virus receptor was not detected in any sample. Southern blot analysis indicated single clonal proliferation of tumor cells. These findings confirm and extend the results of Shibata et al. They also indicate that EBV infection might be related to oncogenesis not only in rare gastric cancers that resemble nasopharyngeal lymphoepithelioma but also in typical gastric adenocarcinoma.

KW - Epstein-Barr virus

KW - gastric adenocarcinoma

KW - in situ hybridization

KW - polymerase chain reaction

KW - Southern blot analysis

UR - http://www.scopus.com/inward/record.url?scp=0028911668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028911668&partnerID=8YFLogxK

U2 - 10.1016/0046-8177(95)90056-X

DO - 10.1016/0046-8177(95)90056-X

M3 - Article

VL - 26

SP - 267

EP - 271

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 3

ER -