Epitope-associated and specificity-focused features of EV71-neutralizing antibody repertoires from plasmablasts of infected children

Kuan Ying Arthur Huang, Mei Feng Chen, Yhu Chering Huang, Shin Ru Shih, Cheng Hsun Chiu, Jainn Jim Lin, Jen Ren Wang, Kuo Chien Tsao, Tzou Yien Lin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Protective antibody levels are critical for protection from severe enterovirus 71 infection. However, little is known about the specificities and functional properties of the enterovirus 71-specific antibodies induced by natural infection in humans. Here we characterize 191 plasmablast-derived monoclonal antibodies from three enterovirus 71-infected children, each of whom shows a distinct serological response. Of the 84 enterovirus 71-specific antibodies, neutralizing antibodies that target the rims and floor of the capsid canyon exhibit broad and potent activities at the nanogram level against viruses isolated in 1998-2016. We also find a subset of infected children whose enterovirus 71-specific antibodies are focused on the 3-and 2-fold plateau epitopes localized at the margin of pentamers, and this type of antibody response is associated with lower serum titers against recently circulating strains. Our data provide new insights into the enterovirus 71-specific antibodies induced by natural infection at the serological and clonal levels.

Original languageEnglish
Article number762
JournalNature Communications
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

Enterovirus
Neutralizing Antibodies
antibodies
Epitopes
Antibodies
infectious diseases
Enterovirus Infections
Capsid
Infection
Viruses
Antibody Formation
Monoclonal Antibodies
canyons
viruses
rims
serums
set theory
margins
plateaus
Serum

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Epitope-associated and specificity-focused features of EV71-neutralizing antibody repertoires from plasmablasts of infected children. / Huang, Kuan Ying Arthur; Chen, Mei Feng; Huang, Yhu Chering; Shih, Shin Ru; Chiu, Cheng Hsun; Lin, Jainn Jim; Wang, Jen Ren; Tsao, Kuo Chien; Lin, Tzou Yien.

In: Nature Communications, Vol. 8, No. 1, 762, 01.12.2017.

Research output: Contribution to journalArticle

Huang, Kuan Ying Arthur ; Chen, Mei Feng ; Huang, Yhu Chering ; Shih, Shin Ru ; Chiu, Cheng Hsun ; Lin, Jainn Jim ; Wang, Jen Ren ; Tsao, Kuo Chien ; Lin, Tzou Yien. / Epitope-associated and specificity-focused features of EV71-neutralizing antibody repertoires from plasmablasts of infected children. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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