Epinecidin-1: An orange-spotted grouper antimicrobial peptide that modulates Staphylococcus aureus lipoteichoic acid-induced inflammation in macrophage cells

Bor Chyuan Su, Jyh Yih Chen

Research output: Contribution to journalArticle

Abstract

Orange-spotted grouper (Epinephelus coioides) is among the most economically important of all fish species farmed in Asia. This species expresses an antimicrobial peptide called epinecidin-1 (EPI), which is considered to be a host defense factor due to its strong bacterial killing activity. Antimicrobial peptides usually possess both bacterial killing and immunomodulatory activity, however, the modulatory activity of EPI on Gram-positive bacterial lipoteichoic acids (LTA)-induced inflammation has not been previously reported. In this study, we found that EPI effectively suppressed LTA-induced production of proinflammatory factors in macrophages. Mechanistically, EPI attenuated LTA-induced inflammation by inhibiting Toll-like receptor (TLR) 2 internalization and subsequent downstream signaling (reactive oxygen species, Akt, p38 and Nuclear factor κB). However, protein abundance of TLR2 was not altered by EPI or LTA. Taken together, our findings reveal for the first time that EPI possesses inhibitory activity toward LTA-induced inflammation in macrophages.

Original languageEnglish
Pages (from-to)362-367
Number of pages6
JournalFish and Shellfish Immunology
Volume99
DOIs
Publication statusPublished - Apr 2020

Keywords

  • Antimicrobial peptide
  • Epinecidin-1
  • Inflammation
  • Lipoteichoic acid
  • Macrophage
  • Orange-spotted grouper

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

Fingerprint Dive into the research topics of 'Epinecidin-1: An orange-spotted grouper antimicrobial peptide that modulates Staphylococcus aureus lipoteichoic acid-induced inflammation in macrophage cells'. Together they form a unique fingerprint.

  • Cite this