Epidermal growth factor improved alcohol-induced inflammation in rats

Ya Ling Chen, Hsiang Chi Peng, Yi Ching Hsieh, Suh-Ching Yang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The purpose of this study was to investigate the effects of an epidermal growth factor (EGF) intervention on improving the inflammatory response of rats fed an ethanol-containing diet. Eight-week-old male Wistar rats were divided into ethanol (E) and control (C) groups. Rats in the E group were fed an ethanol liquid diet, while rats in the C group were pair-fed an isoenergetic diet without ethanol. After a 4-week ethanol-induction period, both the C and E group were respectively subdivided into 2 groups: a normal liquid diet without (C group, n=8) or with EGF supplementation (C+EGF, n=8), and the ethanol-containing diet without (E group, n=8) or with EGF supplementation (E+EGF group, n=8). The EGF (30μg/kg body weight/day) intervention period was carried out for the following 8 weeks. At the end of the experiment, activity of aspartate transaminase (AST) and alanine transaminase (ALT) and hepatic levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in group E were significantly higher than those in group C. In addition, alterations in the gut microbiota profile were found in group E. In contrast, activity of AST and ALT and levels of TNF-α, IL-1β, and IL-6 in group E+EGF were significantly lower than those in group E. Significantly lower intestinal permeability and lower numbers of Escherichia coli in the fecal microbial culture were also found in group E+EGF. These results suggest that EGF improved the intestinal integrity by decreasing E.coli colonization and lowering intestinal permeability, which then ameliorated the inflammatory response under chronic ethanol exposure.

Original languageEnglish
Pages (from-to)701-706
Number of pages6
JournalAlcohol
Volume48
Issue number7
DOIs
Publication statusPublished - Nov 1 2014

Fingerprint

Epidermal Growth Factor
Rats
alcohol
Alcohols
Inflammation
Ethanol
Nutrition
Group
Diet
Aspartate Aminotransferases
Alanine Transaminase
Interleukin-1
Escherichia coli
Permeability
Interleukin-6
Tumor Necrosis Factor-alpha
permeability
Liquids
Interleukin-10
Wistar Rats

Keywords

  • Alcoholic liver disease
  • Epidermal growth factor
  • Intestinal integrity
  • Rats

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neurology
  • Toxicology
  • Health(social science)

Cite this

Epidermal growth factor improved alcohol-induced inflammation in rats. / Chen, Ya Ling; Peng, Hsiang Chi; Hsieh, Yi Ching; Yang, Suh-Ching.

In: Alcohol, Vol. 48, No. 7, 01.11.2014, p. 701-706.

Research output: Contribution to journalArticle

Chen, Ya Ling ; Peng, Hsiang Chi ; Hsieh, Yi Ching ; Yang, Suh-Ching. / Epidermal growth factor improved alcohol-induced inflammation in rats. In: Alcohol. 2014 ; Vol. 48, No. 7. pp. 701-706.
@article{45444f858a304d2e8264c1d63da64431,
title = "Epidermal growth factor improved alcohol-induced inflammation in rats",
abstract = "The purpose of this study was to investigate the effects of an epidermal growth factor (EGF) intervention on improving the inflammatory response of rats fed an ethanol-containing diet. Eight-week-old male Wistar rats were divided into ethanol (E) and control (C) groups. Rats in the E group were fed an ethanol liquid diet, while rats in the C group were pair-fed an isoenergetic diet without ethanol. After a 4-week ethanol-induction period, both the C and E group were respectively subdivided into 2 groups: a normal liquid diet without (C group, n=8) or with EGF supplementation (C+EGF, n=8), and the ethanol-containing diet without (E group, n=8) or with EGF supplementation (E+EGF group, n=8). The EGF (30μg/kg body weight/day) intervention period was carried out for the following 8 weeks. At the end of the experiment, activity of aspartate transaminase (AST) and alanine transaminase (ALT) and hepatic levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in group E were significantly higher than those in group C. In addition, alterations in the gut microbiota profile were found in group E. In contrast, activity of AST and ALT and levels of TNF-α, IL-1β, and IL-6 in group E+EGF were significantly lower than those in group E. Significantly lower intestinal permeability and lower numbers of Escherichia coli in the fecal microbial culture were also found in group E+EGF. These results suggest that EGF improved the intestinal integrity by decreasing E.coli colonization and lowering intestinal permeability, which then ameliorated the inflammatory response under chronic ethanol exposure.",
keywords = "Alcoholic liver disease, Epidermal growth factor, Intestinal integrity, Rats",
author = "Chen, {Ya Ling} and Peng, {Hsiang Chi} and Hsieh, {Yi Ching} and Suh-Ching Yang",
year = "2014",
month = "11",
day = "1",
doi = "10.1016/j.alcohol.2014.07.008",
language = "English",
volume = "48",
pages = "701--706",
journal = "Alcohol",
issn = "0741-8329",
publisher = "Elsevier Inc.",
number = "7",

}

TY - JOUR

T1 - Epidermal growth factor improved alcohol-induced inflammation in rats

AU - Chen, Ya Ling

AU - Peng, Hsiang Chi

AU - Hsieh, Yi Ching

AU - Yang, Suh-Ching

PY - 2014/11/1

Y1 - 2014/11/1

N2 - The purpose of this study was to investigate the effects of an epidermal growth factor (EGF) intervention on improving the inflammatory response of rats fed an ethanol-containing diet. Eight-week-old male Wistar rats were divided into ethanol (E) and control (C) groups. Rats in the E group were fed an ethanol liquid diet, while rats in the C group were pair-fed an isoenergetic diet without ethanol. After a 4-week ethanol-induction period, both the C and E group were respectively subdivided into 2 groups: a normal liquid diet without (C group, n=8) or with EGF supplementation (C+EGF, n=8), and the ethanol-containing diet without (E group, n=8) or with EGF supplementation (E+EGF group, n=8). The EGF (30μg/kg body weight/day) intervention period was carried out for the following 8 weeks. At the end of the experiment, activity of aspartate transaminase (AST) and alanine transaminase (ALT) and hepatic levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in group E were significantly higher than those in group C. In addition, alterations in the gut microbiota profile were found in group E. In contrast, activity of AST and ALT and levels of TNF-α, IL-1β, and IL-6 in group E+EGF were significantly lower than those in group E. Significantly lower intestinal permeability and lower numbers of Escherichia coli in the fecal microbial culture were also found in group E+EGF. These results suggest that EGF improved the intestinal integrity by decreasing E.coli colonization and lowering intestinal permeability, which then ameliorated the inflammatory response under chronic ethanol exposure.

AB - The purpose of this study was to investigate the effects of an epidermal growth factor (EGF) intervention on improving the inflammatory response of rats fed an ethanol-containing diet. Eight-week-old male Wistar rats were divided into ethanol (E) and control (C) groups. Rats in the E group were fed an ethanol liquid diet, while rats in the C group were pair-fed an isoenergetic diet without ethanol. After a 4-week ethanol-induction period, both the C and E group were respectively subdivided into 2 groups: a normal liquid diet without (C group, n=8) or with EGF supplementation (C+EGF, n=8), and the ethanol-containing diet without (E group, n=8) or with EGF supplementation (E+EGF group, n=8). The EGF (30μg/kg body weight/day) intervention period was carried out for the following 8 weeks. At the end of the experiment, activity of aspartate transaminase (AST) and alanine transaminase (ALT) and hepatic levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 in group E were significantly higher than those in group C. In addition, alterations in the gut microbiota profile were found in group E. In contrast, activity of AST and ALT and levels of TNF-α, IL-1β, and IL-6 in group E+EGF were significantly lower than those in group E. Significantly lower intestinal permeability and lower numbers of Escherichia coli in the fecal microbial culture were also found in group E+EGF. These results suggest that EGF improved the intestinal integrity by decreasing E.coli colonization and lowering intestinal permeability, which then ameliorated the inflammatory response under chronic ethanol exposure.

KW - Alcoholic liver disease

KW - Epidermal growth factor

KW - Intestinal integrity

KW - Rats

UR - http://www.scopus.com/inward/record.url?scp=84908030659&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908030659&partnerID=8YFLogxK

U2 - 10.1016/j.alcohol.2014.07.008

DO - 10.1016/j.alcohol.2014.07.008

M3 - Article

C2 - 25174268

AN - SCOPUS:84908030659

VL - 48

SP - 701

EP - 706

JO - Alcohol

JF - Alcohol

SN - 0741-8329

IS - 7

ER -