Epidermal growth factor enhances transcription of human arachidonate 12-lipoxygenase in A431 cells

Yi Wen Liu, Ben Kuen Chen, Ching Jiunn Chen, Toshiya Arakawa, Tanihiro Yoshimoto, Shozo Yamamoto, Wen Chang Chang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Epidermal growth factor (EGF), determined by immunoprecipitation and Western blot analysis, increased both enzyme activity and protein level of 12-lipoxygenase in the solubilized microsomes of human epidermoid carcinoma A431 cells, respectively. The EGF-induced expression of 12-lipoxygenase mRNA was inhibited by transcription inhibitors such as actinomycin D and 5,6-dichlorobenzimidazole riboside. Promoters of different lengths for human 12-lipoxygenase gene were used to prepare the luciferase fusion vectors. These construct plasmids were transiently transfected into A431 cells, and the induction of luciferase expression by EGF was examined. A 4- to 6-fold increase in luciferase reporter activity stimulated by EGF for 18 h treatment was observed in plasmids with the 5'-flanking region length of -951 bp and that of -224 bp upstream from translation starting site. The time-dependent induction of luciferase activity by EGF paralleled the EGF-induced enzyme activity and expression of 12-lipoxygenase protein. Taken together, the results of this study indicate that EGF enhanced the transcription of the human 12-lipoxygenase gene, resulting in an increase in the amount and activity of 12-lipoxygenase.

Original languageEnglish
Pages (from-to)38-46
Number of pages9
JournalBiochimica et Biophysica Acta - Lipids and Lipid Metabolism
Volume1344
Issue number1
DOIs
Publication statusPublished - Jan 7 1997
Externally publishedYes

Fingerprint

Arachidonate 12-Lipoxygenase
Transcription
Epidermal Growth Factor
Luciferases
Enzyme activity
Plasmids
Genes
Dichlororibofuranosylbenzimidazole
5' Flanking Region
Dactinomycin
Enzymes
Microsomes
human ALOX12 protein
Immunoprecipitation
Squamous Cell Carcinoma
Proteins
Fusion reactions
Western Blotting
Messenger RNA

Keywords

  • 12-Lipoxygenase
  • Epidermal growth factor
  • Human epidermoid carcinoma A431 cell
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Biophysics

Cite this

Epidermal growth factor enhances transcription of human arachidonate 12-lipoxygenase in A431 cells. / Liu, Yi Wen; Chen, Ben Kuen; Chen, Ching Jiunn; Arakawa, Toshiya; Yoshimoto, Tanihiro; Yamamoto, Shozo; Chang, Wen Chang.

In: Biochimica et Biophysica Acta - Lipids and Lipid Metabolism, Vol. 1344, No. 1, 07.01.1997, p. 38-46.

Research output: Contribution to journalArticle

Liu, Yi Wen ; Chen, Ben Kuen ; Chen, Ching Jiunn ; Arakawa, Toshiya ; Yoshimoto, Tanihiro ; Yamamoto, Shozo ; Chang, Wen Chang. / Epidermal growth factor enhances transcription of human arachidonate 12-lipoxygenase in A431 cells. In: Biochimica et Biophysica Acta - Lipids and Lipid Metabolism. 1997 ; Vol. 1344, No. 1. pp. 38-46.
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