EphA2 is a biomarker of hMSCs derived from human placenta and umbilical cord

Shih Pei Shen, Wei Ting Liu, Yun Lin, Yuan Tsung Li, Chih Hao Chang, Fung Wei Chang, Le Ming Wang, Sen Wen Teng, Yogi Hsuan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective The heterogeneous nature of mesenchymal stem cells (MSCs) and the absence of known MSC-specific biomarkers make it challenging to define MSC phenotypes and characteristics. In this study, we compared the phenotypic and functional features of human placenta-derived MSCs with those of human dermal fibroblasts in vitro in order to identify a biomarker that can be used to increase the purity of MSCs in a primary culture of placenta-derived cells. Materials and methods Liquid chromatography-tandem mass spectrometry analysis was used to analyze and compare the proteome of human placenta-derived MSCs with that of fibroblasts. Quantitative real-time polymerase chain reaction, immunofluorescence, and flow cytometry were used to determine expression levels of EphA2 in placenta-derived MSCs. EphA2-positive cells were enriched by magnetic-activated cell sorting or with a cell sorter. An shRNA-mediated EphA2 knockdown was used to assess the role of EphA2 in MSC response to Tumor necrosis factor (TNF)-α stimulation. Results Analysis of proteomics data from MSCs and fibroblasts resulted in the identification of the EphA2 surface protein biomarker, which could reliably distinguish MSCs from fibroblasts. EphA2 was significantly upregulated in placenta-derived MSCs when compared to fibroblasts. EphA2 played an important role in MSC migration in response to inflammatory stimuli, such as TNF-α. EphA2-enriched MSCs were also more responsive to inflammatory stimuli in vitro when compared to unsorted MSCs, indicating a role for EphA2 in the immunomodulatory functionality of MSCs. Conclusion EphA2 can be used to distinguish and isolate MSCs from a primary culture of placenta-derived cells. EphA2-sorted MSCs exhibited superior responsiveness to TNF-α signaling in an inflammatory environment compared with unsorted MSCs or MSC-like cells.

Original languageEnglish
Pages (from-to)749-756
Number of pages8
JournalTaiwanese Journal of Obstetrics and Gynecology
Volume54
Issue number6
DOIs
Publication statusPublished - Dec 1 2015

Keywords

  • biomarker
  • EphA2
  • fibroblast
  • immunomodulation
  • mesenchymal stem cells

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Fingerprint Dive into the research topics of 'EphA2 is a biomarker of hMSCs derived from human placenta and umbilical cord'. Together they form a unique fingerprint.

  • Cite this

    Shen, S. P., Liu, W. T., Lin, Y., Li, Y. T., Chang, C. H., Chang, F. W., Wang, L. M., Teng, S. W., & Hsuan, Y. (2015). EphA2 is a biomarker of hMSCs derived from human placenta and umbilical cord. Taiwanese Journal of Obstetrics and Gynecology, 54(6), 749-756. https://doi.org/10.1016/j.tjog.2015.10.012