Eotaxin-2 increased toll-like receptor 4 expression in endothelial cells in vitro and exacerbates high-cholesterol diet-induced atherogenesis in vivo

Chien Sung Tsai, Chun Yao Huang, Chien Ho Chen, Yi Wen Lin, Chun Ming Shih, Nai Wen Tsao, Kuang Hsing Chiang, Chi Yuan Lee, Hellen Jeng, Feng Yen Lin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Eotaxin-2 is a potent chemoattractant. High concentration of eotaxin-2 triggers the inflammation and tumor metastasis. Inhibition of eotaxin-2 may protect experimental atherogenesis although the mechanism is still unclear. Toll-like receptor 4 (TLR4) plays a major role mediating vascular inflammation, which is related to atherogenesis. In the results, we demonstrated that eotaxin-2 potentially impairs the tube formation capacity of human coronary artery endothelial cells (HCAECs). Eotaxin-2 augments the monocytic adhesion in lipopolysaccharides (LPS)-induced HCAECs, and which were reversed by TLR4 siRNA. Thus this study was conducted to investigate whether eotaxin-2 increases TLR4 expression, and then enhances the sensitivity of cells to antigen stimulation in HCAECs, which medi­ates the increasing of the development of serious atherosclerosis. In fact, we showed that JNK/SAPK, p38 MAPK, and ERK1/2 activation contribute to the transcriptional signaling pathway, JNK/SAPK and p38 MAPK regulate post-transcriptional modification, as well as protein-trafficking pathway in eotaxin-2-treated HCAECs TLR4 expression. RNA binding proteins, such as human antigen R (HuR) and tristetraprolin (TTP) mediate stability of TLR4 mRNA and chaperone, such as PRAT4A (a protein associated with TLR4) regulate trafficking of TLR4 protein might confer eotaxin-2 responsiveness. Eotaxin-2 administration led to a significant elevation of high cholesterol diet-induced atherosclerosis, and of TLR4 expression in B6.129S7-Ldlrtm1Her/J but not Ldlr-/--/-/ Tlr4-/- mice. Our results revealed that eotaxin-2 induced overexpression TLR4 via mitogen-activated protein kinases (MAPK) signaling pathways, RNA binding proteins-mediated mRNA stabilization, and PRAT4A-regulated trafficking in HCAECs. These effects may lead to amplification of inflammatory responses contribute to the pathogenesis of cardiovascular disorders.

Original languageEnglish
Pages (from-to)5338-5353
Number of pages16
JournalAmerican Journal of Translational Research
Volume8
Issue number12
Publication statusPublished - 2016
Externally publishedYes

Fingerprint

Chemokine CCL24
Toll-Like Receptor 4
Endothelial cells
Nutrition
Atherosclerosis
Endothelial Cells
Cholesterol
Diet
Coronary Vessels
RNA-Binding Proteins
p38 Mitogen-Activated Protein Kinases
Tristetraprolin
Human Trafficking
In Vitro Techniques
Inflammation
Antigens
Messenger RNA
Proteins
MAP Kinase Signaling System
Mitogen-Activated Protein Kinase 1

Keywords

  • Atherosclerosis
  • Endothelial cells
  • Eotaxin-2
  • Toll-like receptor 4

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Medicine
  • Clinical Biochemistry
  • Cancer Research

Cite this

Eotaxin-2 increased toll-like receptor 4 expression in endothelial cells in vitro and exacerbates high-cholesterol diet-induced atherogenesis in vivo. / Tsai, Chien Sung; Huang, Chun Yao; Chen, Chien Ho; Lin, Yi Wen; Shih, Chun Ming; Tsao, Nai Wen; Chiang, Kuang Hsing; Lee, Chi Yuan; Jeng, Hellen; Lin, Feng Yen.

In: American Journal of Translational Research, Vol. 8, No. 12, 2016, p. 5338-5353.

Research output: Contribution to journalArticle

Tsai, Chien Sung ; Huang, Chun Yao ; Chen, Chien Ho ; Lin, Yi Wen ; Shih, Chun Ming ; Tsao, Nai Wen ; Chiang, Kuang Hsing ; Lee, Chi Yuan ; Jeng, Hellen ; Lin, Feng Yen. / Eotaxin-2 increased toll-like receptor 4 expression in endothelial cells in vitro and exacerbates high-cholesterol diet-induced atherogenesis in vivo. In: American Journal of Translational Research. 2016 ; Vol. 8, No. 12. pp. 5338-5353.
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AU - Lin, Yi Wen

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