Eosinophils from asthmatics release IL-5 in an autocrine fashion to prevent apoptosis through upregulation of Bcl-2 expression

Chien Da Huang, Chun Hua Wang, Chien Ying Liu, Shu Min Lin, Chun Liang Chou, Wen Te Liu, Horng Chyuan Lin, Han Pin Kuo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Interleukin (IL)-5 plays an important role in maintaining the survival of eosinophils via the specific α-subunit of its receptor. Apoptosis, a form of programmed cell death, is thought to represent a mechanism that promotes the resolution of eosinophilic inflammation in asthma. The aim of our present study is to investigate whether IL-5 acts in an autocrine fashion on eosinophil apoptosis in asthmatics. Immunoreactivities of intracellular IL-5 and IL-5 receptor α-subunit (Rα) were detected uniquely Ion the eosinophils. The magnitude of IL-5 and IL-5 Rα expression on eosinophils was significantly higher in asthmatics than that of normal subjects (p <0.05) determined by flow cytometry. Apoptosis of eosinophils was measured by the propidium iodide staining method and DNA ladder. The percent of apoptotic eosinophils from asthmatics was significantly increased by coincubation with anti-hIL-5 Rα Ab (0.1, 0.5, and 2.5 μg/mL) for 1, 2, or 16 hours than was those of corresponding controls (p <0.05, n = 8). However, there was no significant effect of anti-hIL-5 Rα Ab on eosinophil apoptosis in normal subjects. Furthermore, the expression of B-cell lymphoma-2 (Bcl-2) proteins was significantly inhibited by the anti-hIL-5 Rα Ab or antisense IL-5 oligonucleotides in asthmatics (p <0.05, n = 8), but there was no significant change in eosinophils from normal subjects. This study demonstrates that eosinophils from asthmatics release IL-5 in an autocrine fashion to act on their own IL-5 receptors in prevention of apoptosis through the upregulation of Bcl-2 expression.

Original languageEnglish
Pages (from-to)395-403
Number of pages9
JournalJournal of Asthma
Volume42
Issue number5
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Interleukin-5
B-Cell Lymphoma
Eosinophils
Up-Regulation
Apoptosis
Interleukin-5 Receptors
Propidium
Oligonucleotides
Flow Cytometry
Cell Death
Asthma
Ions
Staining and Labeling
Inflammation
DNA

Keywords

  • Apoptosis
  • Asthma
  • Autocrine
  • Bcl-2
  • Eosinophil
  • Interleukin-5

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Eosinophils from asthmatics release IL-5 in an autocrine fashion to prevent apoptosis through upregulation of Bcl-2 expression. / Huang, Chien Da; Wang, Chun Hua; Liu, Chien Ying; Lin, Shu Min; Chou, Chun Liang; Liu, Wen Te; Lin, Horng Chyuan; Kuo, Han Pin.

In: Journal of Asthma, Vol. 42, No. 5, 2005, p. 395-403.

Research output: Contribution to journalArticle

Huang, Chien Da ; Wang, Chun Hua ; Liu, Chien Ying ; Lin, Shu Min ; Chou, Chun Liang ; Liu, Wen Te ; Lin, Horng Chyuan ; Kuo, Han Pin. / Eosinophils from asthmatics release IL-5 in an autocrine fashion to prevent apoptosis through upregulation of Bcl-2 expression. In: Journal of Asthma. 2005 ; Vol. 42, No. 5. pp. 395-403.
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AU - Chou, Chun Liang

AU - Liu, Wen Te

AU - Lin, Horng Chyuan

AU - Kuo, Han Pin

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AB - Interleukin (IL)-5 plays an important role in maintaining the survival of eosinophils via the specific α-subunit of its receptor. Apoptosis, a form of programmed cell death, is thought to represent a mechanism that promotes the resolution of eosinophilic inflammation in asthma. The aim of our present study is to investigate whether IL-5 acts in an autocrine fashion on eosinophil apoptosis in asthmatics. Immunoreactivities of intracellular IL-5 and IL-5 receptor α-subunit (Rα) were detected uniquely Ion the eosinophils. The magnitude of IL-5 and IL-5 Rα expression on eosinophils was significantly higher in asthmatics than that of normal subjects (p <0.05) determined by flow cytometry. Apoptosis of eosinophils was measured by the propidium iodide staining method and DNA ladder. The percent of apoptotic eosinophils from asthmatics was significantly increased by coincubation with anti-hIL-5 Rα Ab (0.1, 0.5, and 2.5 μg/mL) for 1, 2, or 16 hours than was those of corresponding controls (p <0.05, n = 8). However, there was no significant effect of anti-hIL-5 Rα Ab on eosinophil apoptosis in normal subjects. Furthermore, the expression of B-cell lymphoma-2 (Bcl-2) proteins was significantly inhibited by the anti-hIL-5 Rα Ab or antisense IL-5 oligonucleotides in asthmatics (p <0.05, n = 8), but there was no significant change in eosinophils from normal subjects. This study demonstrates that eosinophils from asthmatics release IL-5 in an autocrine fashion to act on their own IL-5 receptors in prevention of apoptosis through the upregulation of Bcl-2 expression.

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