Enzyme-crosslinked gelatin hydrogel with adipose-derived stem cell spheroid facilitating wound repair in the murine burn model

Ting Yu Lu, Kai Fu Yu, Shuo Hsiu Kuo, Nai Chen Cheng, Er Yuan Chuang, Jia Shing Yu

Research output: Contribution to journalArticlepeer-review

Abstract

Engineered skin that can facilitate tissue repair has been a great advance in the field of wound healing. A well-designed dressing material together with active biological cues such as cells or growth factors can overcome the limitation of using auto-grafts from patients. Recently, many studies showed that human adipose-derived stem cells (hASCs) can be used to promote wound healing and skin tissue engineering. hASCs have already been widely applied for clinical trials. hASCs can be harvested abundantly because they can be easily isolated from fat tissue known as the stromal vascular fraction (SVF). On the other hand, increasing studies have proven that cells from spheroids can better simulate the biological microenvironment and can enhance the expression of stemness markers. However, a three-dimensional (3D) scaffold that can harbor implanted cells and can serve as a skin-repaired substitute still suffers from deficiency. In this study, we applied a gelatin/microbial transglutaminase (mTG) hydrogel to encapsulate hASC spheroids to evaluate the performance of 3D cells on skin wound healing. The results showed that the hydrogel is not toxic to the wound and that cell spheroids have significantly improved wound healing compared to cell suspension encapsulated in the hydrogel. Additionally, a hydrogel with cell spheroids was much more effective than other groups in angiogenesis since the cell spheroid has the possibility of cell–cell signaling to promote vascular generation.

Original languageEnglish
Article number2997
Pages (from-to)1-13
Number of pages13
JournalPolymers
Volume12
Issue number12
DOIs
Publication statusPublished - Dec 2020

Keywords

  • Cell spheroid
  • Gelatin
  • Human adipose-derived stem cells
  • Hydrogel
  • Wound healing

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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