Enzymatic and immunological comparison of alanine

glyoxylate aminotransferases from different fish and mammalian livers

Tomoo Noguchi, Satoko Fujiwara, Yoshikazu Takada, Toshio Mori, Miyota Nagano, Nobuhiro Hanada, Eiichi Saeki, Oota Yasuo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

1. 1. Alanine: glyoxylate aminotransferase was highly purified and characterized from mackerel liver. The purified enzyme had a mol. wt of approx. 200,000 with four identical subunits. It was specific for l-alanine and l-serine with glyoxylate and for l-serine with pyruvate as amino acceptor. 2. 2. The mackerel enzyme was similar to partially purified alanine: glyoxylate aminotransferases from other fish (sardine, gopher gray rock cod and yellow mackerel) liver with respect to mol. wts and substrate specificity. 3. 3. These fish enzymes were similar to mammalian liver alanine: glyoxylate aminotransferases 1 in substrate specificity and to mammalian alanine:glyoxylate aminotransferases 2 in mol. wts. 4. 4. An immunological cross-reactivity of hepatic alanine:glyoxylate aminotransferase was observed between mackerel and other fishes but not between mackerel and mammals.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume77
Issue number2
DOIs
Publication statusPublished - 1984
Externally publishedYes

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Perciformes
Liver
Fish
Fishes
Substrate Specificity
Serine
Gophers
Enzymes
Gadiformes
Mammals
Substrates
Pyruvic Acid
Alanine
Rocks
Alanine-glyoxylate transaminase

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

Enzymatic and immunological comparison of alanine : glyoxylate aminotransferases from different fish and mammalian livers. / Noguchi, Tomoo; Fujiwara, Satoko; Takada, Yoshikazu; Mori, Toshio; Nagano, Miyota; Hanada, Nobuhiro; Saeki, Eiichi; Yasuo, Oota.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 77, No. 2, 1984, p. 279-283.

Research output: Contribution to journalArticle

Noguchi, Tomoo ; Fujiwara, Satoko ; Takada, Yoshikazu ; Mori, Toshio ; Nagano, Miyota ; Hanada, Nobuhiro ; Saeki, Eiichi ; Yasuo, Oota. / Enzymatic and immunological comparison of alanine : glyoxylate aminotransferases from different fish and mammalian livers. In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 1984 ; Vol. 77, No. 2. pp. 279-283.
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