Enterovirus 71 infection cleaves a negative regulator for viral internal ribosomal entry site-driven translation

Li Lien Chen, Yu An Kung, Kuo Feng Weng, Jing Yi Lin, Jim Tong Horng, Shin Ru Shih

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Far-upstream element-binding protein 2 (FBP2) is an internal ribosomal entry site (IRES) trans-acting factor (ITAF) that negatively regulates enterovirus 71 (EV71) translation. This study shows that EV71 infection cleaved FBP2. Live EV71 and the EV71 replicon (but not UV-inactivated virus particles) induced FBP2 cleavage, suggesting that viral replication results in FBP2 cleavage. The results also showed that virus-induced proteasome, autophagy, and caspase activity co-contribute to EV71-induced FBP2 cleavage. Using FLAG-fused FBP2, we mapped the potential cleavage fragments of FBP2 in infected cells. We also found that FBP2 altered its function when its carboxyl terminus was cleaved. This study presents a mechanism for virus-induced cellular events to cleave a negative regulator for viral IRES-driven translation.

Original languageEnglish
Pages (from-to)3828-3838
Number of pages11
JournalJournal of Virology
Volume87
Issue number7
DOIs
Publication statusPublished - Apr 2013
Externally publishedYes

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Enterovirus Infections
Enterovirus
translation (genetics)
binding proteins
Carrier Proteins
infection
Viruses
replicon
viruses
Replicon
Trans-Activators
autophagy
caspases
Autophagy
proteasome endopeptidase complex
Proteasome Endopeptidase Complex
Caspases
virus replication
virion
Virion

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Enterovirus 71 infection cleaves a negative regulator for viral internal ribosomal entry site-driven translation. / Chen, Li Lien; Kung, Yu An; Weng, Kuo Feng; Lin, Jing Yi; Horng, Jim Tong; Shih, Shin Ru.

In: Journal of Virology, Vol. 87, No. 7, 04.2013, p. 3828-3838.

Research output: Contribution to journalArticle

Chen, Li Lien ; Kung, Yu An ; Weng, Kuo Feng ; Lin, Jing Yi ; Horng, Jim Tong ; Shih, Shin Ru. / Enterovirus 71 infection cleaves a negative regulator for viral internal ribosomal entry site-driven translation. In: Journal of Virology. 2013 ; Vol. 87, No. 7. pp. 3828-3838.
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