Enrichment of the antibodies against the C-terminus of Taiwan cobra cobrotoxin using dimeric glutaraldehyde-modified toxin as an immunogen

Long Sen Chang, Rose Lin, Ku Chung Chen, Chun Chang Chang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The repertoire of antibodies producing by immunizing rabbits with cobrotoxin and dimeric glutaraldehyde-modified cobrotoxin (dGA-cobrotoxin) was analyzed by studying the immunoreactivity of the two antibody preparations toward cobrotoxin, GA-cobrotoxin and recombinant cobrotoxin. The results of enzyme-linked immunoassay revealed that the two antibody preparations exhibited a higher reactivity against their cognate antigen. Moreover, different behavior was observed for the reactivity of the two antibody preparations against GA-cobrotoxin and recombinant cobrotoxin. Notably, distortion of disulfide linkages at the C-terminus resulted in a reduced decrease in the antigenic activity of recombinant cobrotoxin toward anti-cobrotoxin antibodies compared to anti-dGA-cobrotoxin antibodies. Affinity purification of the antibodies against the C-terminus of cobrotoxin revealed that its amount represented 77% and 35.5% of the total anti-dGA-cobrotoxin antibodies and the total anti-cobrotoxin antibodies, respectively. These findings suggest that the antibody preparation elicited by dGA-cobrotoxin enriches the content of antibodies recognizes the C-terminal region of native cobrotoxin.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalToxicon
Volume41
Issue number2
DOIs
Publication statusPublished - Feb 1 2003
Externally publishedYes

Keywords

  • Cobrotoxin
  • Enzyme-linked immunoassay
  • Glutaraldehyde cross-linking
  • Repertoire of antibodies

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Enrichment of the antibodies against the C-terminus of Taiwan cobra cobrotoxin using dimeric glutaraldehyde-modified toxin as an immunogen'. Together they form a unique fingerprint.

  • Cite this