Enhancing bright-field image of microorganisms by local plasmon of Ag nanoparticle array

Hui Hsin Hsiao, Po Chun Yeh, Huai Hsien Wang, Tian You Cheng, Hung Chun Chang, Yuh Lin Wang, Juen Kai Wang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Inspecting biological cells with bright-field light microscopy often engenders a challenge, owing to their optical transparency. We show that imaging contrast can be greatly enhanced as yeast cells are placed on a silver nano-particle array. Its near- and far-field traits, revealed by electrodynamic simulations, illustrate that the enhancement is attributed to the sensitivity of its plasmonic characteristics to the attached cells. This study demonstrates that the silver nanoparticle array can serve as the agent for concurrently enhancing Raman scattering and imaging contrast of microorganisms for identification and examination.

Original languageEnglish
Pages (from-to)1173-1176
Number of pages4
JournalOptics Letters
Volume39
Issue number5
DOIs
Publication statusPublished - Mar 1 2014
Externally publishedYes

Fingerprint

microorganisms
silver
nanoparticles
yeast
electrodynamics
far fields
near fields
examination
Raman spectra
microscopy
augmentation
sensitivity
cells
simulation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Hsiao, H. H., Yeh, P. C., Wang, H. H., Cheng, T. Y., Chang, H. C., Wang, Y. L., & Wang, J. K. (2014). Enhancing bright-field image of microorganisms by local plasmon of Ag nanoparticle array. Optics Letters, 39(5), 1173-1176. https://doi.org/10.1364/OL.39.001173

Enhancing bright-field image of microorganisms by local plasmon of Ag nanoparticle array. / Hsiao, Hui Hsin; Yeh, Po Chun; Wang, Huai Hsien; Cheng, Tian You; Chang, Hung Chun; Wang, Yuh Lin; Wang, Juen Kai.

In: Optics Letters, Vol. 39, No. 5, 01.03.2014, p. 1173-1176.

Research output: Contribution to journalArticle

Hsiao, HH, Yeh, PC, Wang, HH, Cheng, TY, Chang, HC, Wang, YL & Wang, JK 2014, 'Enhancing bright-field image of microorganisms by local plasmon of Ag nanoparticle array', Optics Letters, vol. 39, no. 5, pp. 1173-1176. https://doi.org/10.1364/OL.39.001173
Hsiao HH, Yeh PC, Wang HH, Cheng TY, Chang HC, Wang YL et al. Enhancing bright-field image of microorganisms by local plasmon of Ag nanoparticle array. Optics Letters. 2014 Mar 1;39(5):1173-1176. https://doi.org/10.1364/OL.39.001173
Hsiao, Hui Hsin ; Yeh, Po Chun ; Wang, Huai Hsien ; Cheng, Tian You ; Chang, Hung Chun ; Wang, Yuh Lin ; Wang, Juen Kai. / Enhancing bright-field image of microorganisms by local plasmon of Ag nanoparticle array. In: Optics Letters. 2014 ; Vol. 39, No. 5. pp. 1173-1176.
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