Enhancement of Transactivation Activity of Rta of Epstein-Barr Virus by RanBPM

Li Kwan Chang, Shih Tung Liu, Chung Wen Kuo, Wen Hung Wang, Jian Ying Chuang, Elisabetta Bianchi, Yi Ren Hong

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) expresses the immediate-early protein Rta to activate the transcription of EBV lytic genes and the lytic cycle. We show that RanBPM acts as a binding partner of Rta in yeast two-hybrid analysis. The binding was confirmed by glutathione-S-transferase pull-down assay. A coimmunoprecipitation experiment and confocal microscopy revealed that RanBPM and Rta interact in vivo and colocalize in the nucleus. The interaction appears to involve the SPRY domain in RanBPM and the region between amino acid residues 416 to 476 in Rta. The interaction promotes the transactivation activity of Rta in activating the transcription of BMLF1 and p21 in transient transfection assays. Additionally, RanBPM interacts with SUMO-E2 (Ubc9) to promote sumoylation of Rta by SUMO-1. This fact explains why the expression of RanBPM enhances the transactivation activity of Rta. Taken together, the present results indicate a new role of RanBPM in regulating a viral protein that is critical to EBV lytic activation.

Original languageEnglish
Pages (from-to)231-242
Number of pages12
JournalJournal of Molecular Biology
Volume379
Issue number2
DOIs
Publication statusPublished - May 29 2008
Externally publishedYes

Fingerprint

Human Herpesvirus 4
Transcriptional Activation
Immediate-Early Proteins
Sumoylation
Virus Activation
Viral Proteins
Glutathione Transferase
Confocal Microscopy
Transfection
Ran binding protein 9
Yeasts
Amino Acids
Genes

Keywords

  • Epstein-Barr virus
  • RanBPM
  • Rta

ASJC Scopus subject areas

  • Virology

Cite this

Enhancement of Transactivation Activity of Rta of Epstein-Barr Virus by RanBPM. / Chang, Li Kwan; Liu, Shih Tung; Kuo, Chung Wen; Wang, Wen Hung; Chuang, Jian Ying; Bianchi, Elisabetta; Hong, Yi Ren.

In: Journal of Molecular Biology, Vol. 379, No. 2, 29.05.2008, p. 231-242.

Research output: Contribution to journalArticle

Chang, Li Kwan ; Liu, Shih Tung ; Kuo, Chung Wen ; Wang, Wen Hung ; Chuang, Jian Ying ; Bianchi, Elisabetta ; Hong, Yi Ren. / Enhancement of Transactivation Activity of Rta of Epstein-Barr Virus by RanBPM. In: Journal of Molecular Biology. 2008 ; Vol. 379, No. 2. pp. 231-242.
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