Enhancement of osteoblastic-like cell activity by glow discharge plasma surface modified hydroxyapatite/β-tricalcium phosphate bone substitute

Eisner Salamanca, Yu Hwa Pan, Aileen I. Tsai, Pei Ying Lin, Ching Kai Lin, Haw Ming Huang, Nai Chia Teng, Peter D. Wang, Wei Jen Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Glowdischarge plasma (GDP) treatments of biomaterials, such as hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) composites, produce surfaces with fewer contaminants and may facilitate cell attachment and enhance bone regeneration. Thus, in this study we used argon glow discharge plasma (Ar-GDP) treatments to modify HA/β-TCP particle surfaces and investigated the physical and chemical properties of the resulting particles (HA/β-TCP + Ar-GDP). The HA/β-TCP particles were treated with GDP for 15 min in argon gas at room temperature under the following conditions: power: 80 W; frequency: 13.56 MHz; pressure: 100 mTorr. Scanning electron microscope (SEM) observations showed similar rough surfaces of HA/β-TCP + Ar-GDP HA/β-TCP particles, and energy dispersive spectrometry analyses showed that HA/β-TCP surfaces had more contaminants than HA/β-TCP + Ar-GDP surfaces. Ca/P mole ratios in HA/β-TCP and HAβ-TCP + Ar-GDP were 1.34 and 1.58, respectively. Both biomaterials presented maximal intensities of X-ray diffraction patterns at 27° with 600 a.u. At 25° and 40°, HA/β-TCP + Ar-GDP and HA/β-TCP particles had peaks of 200 a.u., which are similar to XRD intensities of human bone. In subsequent comparisons, MG-63 cell viability and differentiation into osteoblast-like cells were assessed on HA/β-TCP and HA/β-TCP + Ar-GDP surfaces, and Ar-GDP treatments led to improved cell growth and alkaline phosphatase activities. The present data indicate that GDP surface treatment modified HA/β-TCP surfaces by eliminating contaminants, and the resulting graft material enhanced bone regeneration.

Original languageEnglish
Article number1347
JournalMaterials
Volume10
Issue number12
DOIs
Publication statusPublished - Nov 23 2017

Fingerprint

Bone Substitutes
Glow discharges
Durapatite
Hydroxyapatite
Bone
Phosphates
Argon
Plasmas
Biocompatible Materials
Impurities
tricalcium phosphate
Biomaterials
Osteoblasts
Phosphatases
Cell growth
Grafts
Chemical properties
Diffraction patterns
Spectrometry

Keywords

  • Argon glow discharge plasma
  • Cell viability
  • Differentiation
  • Guided bone regeneration
  • HA/β-TCP
  • Osteoconduction

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Enhancement of osteoblastic-like cell activity by glow discharge plasma surface modified hydroxyapatite/β-tricalcium phosphate bone substitute. / Salamanca, Eisner; Pan, Yu Hwa; Tsai, Aileen I.; Lin, Pei Ying; Lin, Ching Kai; Huang, Haw Ming; Teng, Nai Chia; Wang, Peter D.; Chang, Wei Jen.

In: Materials, Vol. 10, No. 12, 1347, 23.11.2017.

Research output: Contribution to journalArticle

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