Enhancement of glutathione S-transferase using diallyl sulfide from garlic in Hepa-1c1c7 cells

Y. H. Chen, S. K. Wu, M. J. Shieh

Research output: Contribution to journalArticle

Abstract

Modification of the expression of xenobiotic metabolizing enzymes, such as glutathione S-transferase (GST), is thought to be one of the mechanisms of the anticarcinogenic effect of garlic. To obtain further insight into the induction of the GST enzyme and on the anticarcinogenic action of garlic, we examined the effect of diallyl sulfide (DAS), an organosulfur compound derived from garlic, on the induction of GST in the murine hepatoma cells (Hepa-1c1c7), which is sensitive to the xenobiotics. We observed that the GST enzyme activity was enhanced after treatment with 5 mM DAS for 16, 24 and 48 hours. Dose-response experiments demonstrated that the GST enzyme activity increased with increasing concentrations of DAS. Furthermore, Western blotting analysis revealed that three GST isoenzyme proteins, GST-α, GST-μ, and GST-π, tended to increase with increasing DAS concentrations. In summary, the results suggest that GST enzyme activity can be enhanced by DAS in Hepa-1c1c7 cells, and the increased expression of the GST isoenzyme proteins contributed to the augmentation of the corresponding enzyme activity. The increased GST activity may explain, at least in part, the anticarcinogenic effect of garlic.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalNutritional Sciences Journal
Volume25
Issue number1
Publication statusPublished - 2000

Fingerprint

diallyl sulfides
Garlic
garlic
Glutathione Transferase
glutathione transferase
cells
Enzymes
Anticarcinogenic Agents
enzyme activity
anticarcinogenic activity
Xenobiotics
xenobiotics
Isoenzymes
allyl sulfide
isozymes
organic sulfur compounds
Protein S
hepatoma
enzymes
dose response

Keywords

  • Diallyl sulfide
  • Garlic
  • Glutathione S-transferase
  • Hepa-1c1c7

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Enhancement of glutathione S-transferase using diallyl sulfide from garlic in Hepa-1c1c7 cells. / Chen, Y. H.; Wu, S. K.; Shieh, M. J.

In: Nutritional Sciences Journal, Vol. 25, No. 1, 2000, p. 1-8.

Research output: Contribution to journalArticle

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