Enhancement of beta-catenin in cardiomyocytes suppresses survival protein expression but promotes apoptosis and fibrosis

James C. Lin, Wei Wen Kuo, Rathinasamy Baskaran, Ming Cheng Chen, Tsung Jung Ho, Ray Jade Chen, Ya Fang Chen, Viswanadha Vijaya Padma, Ing Shiow Lay, Chih Yang Huang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Beta-catenin has been implicated in cell-cell communication in a wide variety of developmental and physiological processes. Defective Wnt signaling could result in various cardiac and vascular abnormalities. Little is known regarding Wnt/frizzled pathway in cardiomyocyte apoptosis. Methods: In this study, the role of b-catenin in apoptosis was investigated in H9c2 cardiomyocytes and primary cardiomyocytes isolated in diabetic Wistar rats. The cardiomyocytes were transfected with porcine cytomegalovirus (pCMV)-b-catenin plasmid in order to overexpress b-catenin. Results: The transcription factor displayed a significant nuclear localization in Wistar rats with cardiac hypertension. Transfection of b-catenin plasmid induced apoptosis and reduced expression of survival pathway markers in cardiomyocytes in a dose-dependent manner. Furthermore, expression of fibrosis protein markers was upregulated by the overexpression. Conclusions: Taken together, these results revealed that altered Wnt/b-catenin signaling might provoke heart failure.

Original languageEnglish
Pages (from-to)195-205
Number of pages11
JournalCardiology Journal
Volume24
Issue number2
DOIs
Publication statusPublished - 2017

Fingerprint

Catenins
beta Catenin
Cardiac Myocytes
Fibrosis
Apoptosis
Proteins
Wistar Rats
Plasmids
Physiological Phenomena
Wnt Signaling Pathway
Cytomegalovirus
Cell Communication
Transfection
Blood Vessels
Transcription Factors
Swine
Heart Failure
Hypertension

Keywords

  • Apoptosis
  • Cardiomyocytes
  • Fibrosis
  • Survival pathway
  • β-catenin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Enhancement of beta-catenin in cardiomyocytes suppresses survival protein expression but promotes apoptosis and fibrosis. / Lin, James C.; Kuo, Wei Wen; Baskaran, Rathinasamy; Chen, Ming Cheng; Ho, Tsung Jung; Chen, Ray Jade; Chen, Ya Fang; Padma, Viswanadha Vijaya; Lay, Ing Shiow; Huang, Chih Yang.

In: Cardiology Journal, Vol. 24, No. 2, 2017, p. 195-205.

Research output: Contribution to journalArticle

Lin, JC, Kuo, WW, Baskaran, R, Chen, MC, Ho, TJ, Chen, RJ, Chen, YF, Padma, VV, Lay, IS & Huang, CY 2017, 'Enhancement of beta-catenin in cardiomyocytes suppresses survival protein expression but promotes apoptosis and fibrosis', Cardiology Journal, vol. 24, no. 2, pp. 195-205. https://doi.org/10.5603/CJ.a2016.0087
Lin, James C. ; Kuo, Wei Wen ; Baskaran, Rathinasamy ; Chen, Ming Cheng ; Ho, Tsung Jung ; Chen, Ray Jade ; Chen, Ya Fang ; Padma, Viswanadha Vijaya ; Lay, Ing Shiow ; Huang, Chih Yang. / Enhancement of beta-catenin in cardiomyocytes suppresses survival protein expression but promotes apoptosis and fibrosis. In: Cardiology Journal. 2017 ; Vol. 24, No. 2. pp. 195-205.
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AU - Kuo, Wei Wen

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AU - Ho, Tsung Jung

AU - Chen, Ray Jade

AU - Chen, Ya Fang

AU - Padma, Viswanadha Vijaya

AU - Lay, Ing Shiow

AU - Huang, Chih Yang

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