Enhanced intracellular heat shock protein 70 expression of leukocytes and serum interleukins release: Comparison of on-pump and off-pump coronary artery surgery

Chih Yuan Lin, Tung Lin Yang, Gou Jieng Hong, Chi Yuan Li, Feng Yen Lin, Chien Sung Tsai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background Coronary artery bypass grafting (CABG) employing cardiopulmonary bypass (CPB; "on-pump" technique) is known to induce a systemic inflammatory response and heat-shock protein 70 kDa (HSP70) expression. The objective of the present study was to investigate the perioperative intracellular HSP70 expression of leukocytes and serum interleukin (IL) release in CABG conducted with both on-pump and off-pump techniques. Methods Thirty-seven patients referred for elective CABG were enrolled in this study. These patients were categorized into the following three groups: on-pump car- dioplegic arrest (n = 12); on-pump beating heart (n = 13); and off-pump (n = 12). Blood samples were collected at four time points during the perioperative period. Enzyme- linked immunosorbent assay (ELISA) was used to determine the serum level of IL-8, IL-10, IL-12, and HSP70. Flow cytometric analysis of intracellular HSP70 was performed in populations of lymphocytes, monocytes, and granulocytes. Results The clinical outcomes were comparable among the three groups. Elevated serum IL-8, IL-10, IL-12 were found in all three groups during the perioperative period. Serum HSP70 was elevated in all three groups and was significantly lower in the off-pump group than in the on- pump cardioplegic arrest and on-pump beating-heart groups. Heat shock protein-70 expression was found in leukocytes and showed a faster response in monocytes and granulocytes than in lymphocytes. The inflammatory response in the off-pump group was less than in either of the on-pump groups. Conclusions During the perioperative period, activation of inflammatory response was associated with enhanced expression of HSP70 within leukocytes in CABG patients.

Original languageEnglish
Pages (from-to)675-681
Number of pages7
JournalWorld Journal of Surgery
Volume34
Issue number4
DOIs
Publication statusPublished - Apr 2010

Fingerprint

HSP70 Heat-Shock Proteins
Interleukins
Coronary Vessels
Leukocytes
Coronary Artery Bypass
Perioperative Period
Serum
Interleukin-12
Interleukin-8
Granulocytes
Interleukin-10
Monocytes
Lymphocytes
Cardiopulmonary Bypass
Blood Proteins
Enzyme-Linked Immunosorbent Assay
Population

ASJC Scopus subject areas

  • Surgery

Cite this

Enhanced intracellular heat shock protein 70 expression of leukocytes and serum interleukins release : Comparison of on-pump and off-pump coronary artery surgery. / Lin, Chih Yuan; Yang, Tung Lin; Hong, Gou Jieng; Li, Chi Yuan; Lin, Feng Yen; Tsai, Chien Sung.

In: World Journal of Surgery, Vol. 34, No. 4, 04.2010, p. 675-681.

Research output: Contribution to journalArticle

@article{9bdbe78bc3aa42238ddbd3845f41a817,
title = "Enhanced intracellular heat shock protein 70 expression of leukocytes and serum interleukins release: Comparison of on-pump and off-pump coronary artery surgery",
abstract = "Background Coronary artery bypass grafting (CABG) employing cardiopulmonary bypass (CPB; {"}on-pump{"} technique) is known to induce a systemic inflammatory response and heat-shock protein 70 kDa (HSP70) expression. The objective of the present study was to investigate the perioperative intracellular HSP70 expression of leukocytes and serum interleukin (IL) release in CABG conducted with both on-pump and off-pump techniques. Methods Thirty-seven patients referred for elective CABG were enrolled in this study. These patients were categorized into the following three groups: on-pump car- dioplegic arrest (n = 12); on-pump beating heart (n = 13); and off-pump (n = 12). Blood samples were collected at four time points during the perioperative period. Enzyme- linked immunosorbent assay (ELISA) was used to determine the serum level of IL-8, IL-10, IL-12, and HSP70. Flow cytometric analysis of intracellular HSP70 was performed in populations of lymphocytes, monocytes, and granulocytes. Results The clinical outcomes were comparable among the three groups. Elevated serum IL-8, IL-10, IL-12 were found in all three groups during the perioperative period. Serum HSP70 was elevated in all three groups and was significantly lower in the off-pump group than in the on- pump cardioplegic arrest and on-pump beating-heart groups. Heat shock protein-70 expression was found in leukocytes and showed a faster response in monocytes and granulocytes than in lymphocytes. The inflammatory response in the off-pump group was less than in either of the on-pump groups. Conclusions During the perioperative period, activation of inflammatory response was associated with enhanced expression of HSP70 within leukocytes in CABG patients.",
author = "Lin, {Chih Yuan} and Yang, {Tung Lin} and Hong, {Gou Jieng} and Li, {Chi Yuan} and Lin, {Feng Yen} and Tsai, {Chien Sung}",
year = "2010",
month = "4",
doi = "10.1007/s00268-010-0419-2",
language = "English",
volume = "34",
pages = "675--681",
journal = "World Journal of Surgery",
issn = "0364-2313",
publisher = "Springer New York LLC",
number = "4",

}

TY - JOUR

T1 - Enhanced intracellular heat shock protein 70 expression of leukocytes and serum interleukins release

T2 - Comparison of on-pump and off-pump coronary artery surgery

AU - Lin, Chih Yuan

AU - Yang, Tung Lin

AU - Hong, Gou Jieng

AU - Li, Chi Yuan

AU - Lin, Feng Yen

AU - Tsai, Chien Sung

PY - 2010/4

Y1 - 2010/4

N2 - Background Coronary artery bypass grafting (CABG) employing cardiopulmonary bypass (CPB; "on-pump" technique) is known to induce a systemic inflammatory response and heat-shock protein 70 kDa (HSP70) expression. The objective of the present study was to investigate the perioperative intracellular HSP70 expression of leukocytes and serum interleukin (IL) release in CABG conducted with both on-pump and off-pump techniques. Methods Thirty-seven patients referred for elective CABG were enrolled in this study. These patients were categorized into the following three groups: on-pump car- dioplegic arrest (n = 12); on-pump beating heart (n = 13); and off-pump (n = 12). Blood samples were collected at four time points during the perioperative period. Enzyme- linked immunosorbent assay (ELISA) was used to determine the serum level of IL-8, IL-10, IL-12, and HSP70. Flow cytometric analysis of intracellular HSP70 was performed in populations of lymphocytes, monocytes, and granulocytes. Results The clinical outcomes were comparable among the three groups. Elevated serum IL-8, IL-10, IL-12 were found in all three groups during the perioperative period. Serum HSP70 was elevated in all three groups and was significantly lower in the off-pump group than in the on- pump cardioplegic arrest and on-pump beating-heart groups. Heat shock protein-70 expression was found in leukocytes and showed a faster response in monocytes and granulocytes than in lymphocytes. The inflammatory response in the off-pump group was less than in either of the on-pump groups. Conclusions During the perioperative period, activation of inflammatory response was associated with enhanced expression of HSP70 within leukocytes in CABG patients.

AB - Background Coronary artery bypass grafting (CABG) employing cardiopulmonary bypass (CPB; "on-pump" technique) is known to induce a systemic inflammatory response and heat-shock protein 70 kDa (HSP70) expression. The objective of the present study was to investigate the perioperative intracellular HSP70 expression of leukocytes and serum interleukin (IL) release in CABG conducted with both on-pump and off-pump techniques. Methods Thirty-seven patients referred for elective CABG were enrolled in this study. These patients were categorized into the following three groups: on-pump car- dioplegic arrest (n = 12); on-pump beating heart (n = 13); and off-pump (n = 12). Blood samples were collected at four time points during the perioperative period. Enzyme- linked immunosorbent assay (ELISA) was used to determine the serum level of IL-8, IL-10, IL-12, and HSP70. Flow cytometric analysis of intracellular HSP70 was performed in populations of lymphocytes, monocytes, and granulocytes. Results The clinical outcomes were comparable among the three groups. Elevated serum IL-8, IL-10, IL-12 were found in all three groups during the perioperative period. Serum HSP70 was elevated in all three groups and was significantly lower in the off-pump group than in the on- pump cardioplegic arrest and on-pump beating-heart groups. Heat shock protein-70 expression was found in leukocytes and showed a faster response in monocytes and granulocytes than in lymphocytes. The inflammatory response in the off-pump group was less than in either of the on-pump groups. Conclusions During the perioperative period, activation of inflammatory response was associated with enhanced expression of HSP70 within leukocytes in CABG patients.

UR - http://www.scopus.com/inward/record.url?scp=77952427615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952427615&partnerID=8YFLogxK

U2 - 10.1007/s00268-010-0419-2

DO - 10.1007/s00268-010-0419-2

M3 - Article

C2 - 20130867

AN - SCOPUS:77952427615

VL - 34

SP - 675

EP - 681

JO - World Journal of Surgery

JF - World Journal of Surgery

SN - 0364-2313

IS - 4

ER -