Enhanced expression of ROCK in left atrial myocytes of mitral regurgitation: A potential mechanism of myolysis

Huang Chung Chen, Jen Ping Chang, Tzu Hao Chang, Yu Sheng Lin, Yao Kuang Huang, Kuo Li Pan, Chih Yuan Fang, Chien Jen Chen, Wan Chun Ho, Mien Cheng Chen

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Abstract

Background: Severe mitral regurgitation (MR) may cause myolysis in the left atrial myocytes. Myolysis may contribute to atrial enlargement. However, the relationship between Rho-associated kinase (ROCK) and myolysis in the left atrial myocytes of MR patients remain unclear. Methods: This study comprised 22 patients with severe MR [12 with atrial fibrillation (AF) and ten in sinus rhythm]. Left atrial appendage tissues were obtained during surgery. Normal left atrial tissues were purchased. Immunofluorescence histochemical and immunoblotting studies were performed. Results: The expression of ROCK2 in the myolytic left atrial myocytes of MR AF patients (p = 0.009) and MR sinus patients (p = 0.011) were significantly higher than that of the normal subjects. Similarly, the expression of ROCK1 in the myolytic left atrial myocytes of MR AF patients was significantly higher than that of the normal subjects (p = 0.010), and the expression of ROCK1 in the myolytic left atrial myocytes of MR sinus patients was higher than that of the normal subjects (p = 0.091). Immunofluorescence study revealed significant co-localization and juxtaposition of ROCK2 and cleaved caspase-3 in the left atrial myocytes both in the MR AF group (Pearson's coefficient = 0.74 ± 0.03) and the MR sinus group (Pearson's coefficient = 0.73 ± 0.02). Similarly, immunofluorescence study revealed significant co-localization and juxtaposition of ROCK1 and cleaved caspase-3 in the left atrial myocytes both in the MR AF group (Pearson's coefficient = 0.65 ± 0.03) and the MR sinus group (Pearson's coefficient = 0.65 ± 0.03). Correlation analysis demonstrated that there was a significant direct relationship between the expression of ROCK2 in the myolytic left atrial myocytes and left atrial diameter in the MR patients (p = 0.041; r = 0.440). Moreover, the ratio of phosphorylated myosin-binding subunit of myosin light chain phosphatase (pMBS)/total MBS of left atrial tissues was significantly higher in the MR AF group (p <0.04) and the MR sinus group (p <0.04) compared with the normal control group. Conclusions: The enhanced expression of ROCKs might be involved in the myolysis of the left atrial myocytes of MR patients.

Original languageEnglish
Article number33
JournalBMC Cardiovascular Disorders
Volume15
Issue number1
DOIs
Publication statusPublished - May 9 2015

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rho-Associated Kinases
Mitral Valve Insufficiency
Muscle Cells
Atrial Fibrillation
Fluorescent Antibody Technique
Caspase 3
Myosin-Light-Chain Phosphatase
Atrial Appendage
Myosins
Immunoblotting

Keywords

  • Mitral regurgitation
  • Myocyte
  • Myolysis
  • Rho-associated kinase

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Enhanced expression of ROCK in left atrial myocytes of mitral regurgitation : A potential mechanism of myolysis. / Chen, Huang Chung; Chang, Jen Ping; Chang, Tzu Hao; Lin, Yu Sheng; Huang, Yao Kuang; Pan, Kuo Li; Fang, Chih Yuan; Chen, Chien Jen; Ho, Wan Chun; Chen, Mien Cheng.

In: BMC Cardiovascular Disorders, Vol. 15, No. 1, 33, 09.05.2015.

Research output: Contribution to journalArticle

Chen, Huang Chung ; Chang, Jen Ping ; Chang, Tzu Hao ; Lin, Yu Sheng ; Huang, Yao Kuang ; Pan, Kuo Li ; Fang, Chih Yuan ; Chen, Chien Jen ; Ho, Wan Chun ; Chen, Mien Cheng. / Enhanced expression of ROCK in left atrial myocytes of mitral regurgitation : A potential mechanism of myolysis. In: BMC Cardiovascular Disorders. 2015 ; Vol. 15, No. 1.
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abstract = "Background: Severe mitral regurgitation (MR) may cause myolysis in the left atrial myocytes. Myolysis may contribute to atrial enlargement. However, the relationship between Rho-associated kinase (ROCK) and myolysis in the left atrial myocytes of MR patients remain unclear. Methods: This study comprised 22 patients with severe MR [12 with atrial fibrillation (AF) and ten in sinus rhythm]. Left atrial appendage tissues were obtained during surgery. Normal left atrial tissues were purchased. Immunofluorescence histochemical and immunoblotting studies were performed. Results: The expression of ROCK2 in the myolytic left atrial myocytes of MR AF patients (p = 0.009) and MR sinus patients (p = 0.011) were significantly higher than that of the normal subjects. Similarly, the expression of ROCK1 in the myolytic left atrial myocytes of MR AF patients was significantly higher than that of the normal subjects (p = 0.010), and the expression of ROCK1 in the myolytic left atrial myocytes of MR sinus patients was higher than that of the normal subjects (p = 0.091). Immunofluorescence study revealed significant co-localization and juxtaposition of ROCK2 and cleaved caspase-3 in the left atrial myocytes both in the MR AF group (Pearson's coefficient = 0.74 ± 0.03) and the MR sinus group (Pearson's coefficient = 0.73 ± 0.02). Similarly, immunofluorescence study revealed significant co-localization and juxtaposition of ROCK1 and cleaved caspase-3 in the left atrial myocytes both in the MR AF group (Pearson's coefficient = 0.65 ± 0.03) and the MR sinus group (Pearson's coefficient = 0.65 ± 0.03). Correlation analysis demonstrated that there was a significant direct relationship between the expression of ROCK2 in the myolytic left atrial myocytes and left atrial diameter in the MR patients (p = 0.041; r = 0.440). Moreover, the ratio of phosphorylated myosin-binding subunit of myosin light chain phosphatase (pMBS)/total MBS of left atrial tissues was significantly higher in the MR AF group (p <0.04) and the MR sinus group (p <0.04) compared with the normal control group. Conclusions: The enhanced expression of ROCKs might be involved in the myolysis of the left atrial myocytes of MR patients.",
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T1 - Enhanced expression of ROCK in left atrial myocytes of mitral regurgitation

T2 - A potential mechanism of myolysis

AU - Chen, Huang Chung

AU - Chang, Jen Ping

AU - Chang, Tzu Hao

AU - Lin, Yu Sheng

AU - Huang, Yao Kuang

AU - Pan, Kuo Li

AU - Fang, Chih Yuan

AU - Chen, Chien Jen

AU - Ho, Wan Chun

AU - Chen, Mien Cheng

PY - 2015/5/9

Y1 - 2015/5/9

N2 - Background: Severe mitral regurgitation (MR) may cause myolysis in the left atrial myocytes. Myolysis may contribute to atrial enlargement. However, the relationship between Rho-associated kinase (ROCK) and myolysis in the left atrial myocytes of MR patients remain unclear. Methods: This study comprised 22 patients with severe MR [12 with atrial fibrillation (AF) and ten in sinus rhythm]. Left atrial appendage tissues were obtained during surgery. Normal left atrial tissues were purchased. Immunofluorescence histochemical and immunoblotting studies were performed. Results: The expression of ROCK2 in the myolytic left atrial myocytes of MR AF patients (p = 0.009) and MR sinus patients (p = 0.011) were significantly higher than that of the normal subjects. Similarly, the expression of ROCK1 in the myolytic left atrial myocytes of MR AF patients was significantly higher than that of the normal subjects (p = 0.010), and the expression of ROCK1 in the myolytic left atrial myocytes of MR sinus patients was higher than that of the normal subjects (p = 0.091). Immunofluorescence study revealed significant co-localization and juxtaposition of ROCK2 and cleaved caspase-3 in the left atrial myocytes both in the MR AF group (Pearson's coefficient = 0.74 ± 0.03) and the MR sinus group (Pearson's coefficient = 0.73 ± 0.02). Similarly, immunofluorescence study revealed significant co-localization and juxtaposition of ROCK1 and cleaved caspase-3 in the left atrial myocytes both in the MR AF group (Pearson's coefficient = 0.65 ± 0.03) and the MR sinus group (Pearson's coefficient = 0.65 ± 0.03). Correlation analysis demonstrated that there was a significant direct relationship between the expression of ROCK2 in the myolytic left atrial myocytes and left atrial diameter in the MR patients (p = 0.041; r = 0.440). Moreover, the ratio of phosphorylated myosin-binding subunit of myosin light chain phosphatase (pMBS)/total MBS of left atrial tissues was significantly higher in the MR AF group (p <0.04) and the MR sinus group (p <0.04) compared with the normal control group. Conclusions: The enhanced expression of ROCKs might be involved in the myolysis of the left atrial myocytes of MR patients.

AB - Background: Severe mitral regurgitation (MR) may cause myolysis in the left atrial myocytes. Myolysis may contribute to atrial enlargement. However, the relationship between Rho-associated kinase (ROCK) and myolysis in the left atrial myocytes of MR patients remain unclear. Methods: This study comprised 22 patients with severe MR [12 with atrial fibrillation (AF) and ten in sinus rhythm]. Left atrial appendage tissues were obtained during surgery. Normal left atrial tissues were purchased. Immunofluorescence histochemical and immunoblotting studies were performed. Results: The expression of ROCK2 in the myolytic left atrial myocytes of MR AF patients (p = 0.009) and MR sinus patients (p = 0.011) were significantly higher than that of the normal subjects. Similarly, the expression of ROCK1 in the myolytic left atrial myocytes of MR AF patients was significantly higher than that of the normal subjects (p = 0.010), and the expression of ROCK1 in the myolytic left atrial myocytes of MR sinus patients was higher than that of the normal subjects (p = 0.091). Immunofluorescence study revealed significant co-localization and juxtaposition of ROCK2 and cleaved caspase-3 in the left atrial myocytes both in the MR AF group (Pearson's coefficient = 0.74 ± 0.03) and the MR sinus group (Pearson's coefficient = 0.73 ± 0.02). Similarly, immunofluorescence study revealed significant co-localization and juxtaposition of ROCK1 and cleaved caspase-3 in the left atrial myocytes both in the MR AF group (Pearson's coefficient = 0.65 ± 0.03) and the MR sinus group (Pearson's coefficient = 0.65 ± 0.03). Correlation analysis demonstrated that there was a significant direct relationship between the expression of ROCK2 in the myolytic left atrial myocytes and left atrial diameter in the MR patients (p = 0.041; r = 0.440). Moreover, the ratio of phosphorylated myosin-binding subunit of myosin light chain phosphatase (pMBS)/total MBS of left atrial tissues was significantly higher in the MR AF group (p <0.04) and the MR sinus group (p <0.04) compared with the normal control group. Conclusions: The enhanced expression of ROCKs might be involved in the myolysis of the left atrial myocytes of MR patients.

KW - Mitral regurgitation

KW - Myocyte

KW - Myolysis

KW - Rho-associated kinase

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