Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective

May Jywan Tsai, Yi Ming Arthur Chen, Ching Feng Weng, Dann Ying Liou, Hsin Chun Yang, Chien Hung Chen, Roanna I.Hsin Liao, Fu Shan Kuo, Chiuan Wen Chiu, Huai Sheng Kuo, Ming Chao Huang, Yi Lo Lin, Meng Jen Lee, Wen Chun Kuo, Wen Cheng Huang, Henrich Cheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

Glycine N-methyltransferase (GNMT) is the most abundant hepatic methyltransferase and plays important roles in regulating methyl group metabolism. In the central nervous system, GNMT expression is low and its function has not been revealed. The present study examines the effect of GNMT overexpression by adenovirus-mediated transfer in cortical mixed neuron-glial cultures. Infection of adenovirus encoding green fluorescence protein to cultures demonstrates high preference for non-neuronal cells. Optimal GNMT overexpression in cultures by adenoviral GNMT (Ad-GNMT) infection not only induces protein kinase C phosphorylation, but also increases neuronaloligodendroglial survival. Furthermore, these Ad-GNMT-infected cultures are significantly resistant to H2O2 toxicity and lipopolysaccharide stimulation. Conditioned media from Ad-GNMT-infected microglia also significantly enhance neuronal survival. Taken together, enhanced GNMT expression in mixed neuronal-glial cultures is neuroprotective, most likely mediated through non-neuronal cells.

Original languageEnglish
Title of host publicationNeuroprotective Agents
Subtitle of host publicationNinth International Conference
PublisherBlackwell Publishing Inc.
Pages194-203
Number of pages10
ISBN (Print)9781573317771
DOIs
Publication statusPublished - Jan 1 2010
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1199
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

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Keywords

  • Adenovirus
  • GNMT
  • Lipopolysaccharide
  • Neuron-glia interaction

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Tsai, M. J., Chen, Y. M. A., Weng, C. F., Liou, D. Y., Yang, H. C., Chen, C. H., Liao, R. I. H., Kuo, F. S., Chiu, C. W., Kuo, H. S., Huang, M. C., Lin, Y. L., Lee, M. J., Kuo, W. C., Huang, W. C., & Cheng, H. (2010). Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective. In Neuroprotective Agents: Ninth International Conference (pp. 194-203). (Annals of the New York Academy of Sciences; Vol. 1199). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.05169.x