Enhanced Chemosensitivity by Targeting Nanog in Head and Neck Squamous Cell Carcinomas

Chuan En Huang, Cheng Chia Yu, Fang Wei Hu, Ming Yung Chou, Lo Lin Tsai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Chemo-resistance is the major cause of high mortality in head and neck squamous cell carcinomas (HNSCC) in which HNSCC-derived cancer stem cells (CSCs) may be involved. Previously, we enriched a subpopulation of HNSCC-derived spheroid cells (SC) (HNSCC-SC) and identified Nanog as a CSCs marker. The aim of this study was to determine the role of Nanog in the chemosensitivity of HNSCC. The functional and clinicopathological studies of Nanog were investigated in HNSCC cells and specimens. Nanog expression was increased in HNSCC cell lines as compared to a normal oral epithelial cell line. Nanog upregulation in clinical tissues from HNSCC patients with recurrent and metastatic specimens relative to the mRNA levels in the samples from normal or primary tissues were examined. Targeting Nanog in HNSCC-SC significantly inhibited their tumorigenic and CSCs-like abilities and effectively increased the sensitivity of HNSCC-SC to chemotherapeutic drug cisplatin treatment. Targeting Nanog in HNSCC-SC showed a synergistic therapeutic effect with cisplatin. Our results suggest that targeting Nanog may have promising therapeutic potential for HNSCC.

Original languageEnglish
Pages (from-to)14935-14948
Number of pages14
JournalInternational Journal of Molecular Sciences
Volume15
Issue number9
DOIs
Publication statusPublished - Aug 25 2014
Externally publishedYes

Fingerprint

cancer
spheroids
Neoplastic Stem Cells
Stem cells
stem cells
cells
Cisplatin
Carcinoma, squamous cell of head and neck
Epithelial Cells
cultured cells
Tissue
Cell Line
Drug therapy
Therapeutic Uses
mortality
Up-Regulation
markers
Cells
drugs
Messenger RNA

Keywords

  • Cancer stem cells
  • Chemosensitivity
  • Head and neck squamous cell carcinomas
  • Nanog

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

Cite this

Enhanced Chemosensitivity by Targeting Nanog in Head and Neck Squamous Cell Carcinomas. / Huang, Chuan En; Yu, Cheng Chia; Hu, Fang Wei; Chou, Ming Yung; Tsai, Lo Lin.

In: International Journal of Molecular Sciences, Vol. 15, No. 9, 25.08.2014, p. 14935-14948.

Research output: Contribution to journalArticle

Huang, Chuan En ; Yu, Cheng Chia ; Hu, Fang Wei ; Chou, Ming Yung ; Tsai, Lo Lin. / Enhanced Chemosensitivity by Targeting Nanog in Head and Neck Squamous Cell Carcinomas. In: International Journal of Molecular Sciences. 2014 ; Vol. 15, No. 9. pp. 14935-14948.
@article{2861fe28c1724ddaa7aa5df5f07a794d,
title = "Enhanced Chemosensitivity by Targeting Nanog in Head and Neck Squamous Cell Carcinomas",
abstract = "Chemo-resistance is the major cause of high mortality in head and neck squamous cell carcinomas (HNSCC) in which HNSCC-derived cancer stem cells (CSCs) may be involved. Previously, we enriched a subpopulation of HNSCC-derived spheroid cells (SC) (HNSCC-SC) and identified Nanog as a CSCs marker. The aim of this study was to determine the role of Nanog in the chemosensitivity of HNSCC. The functional and clinicopathological studies of Nanog were investigated in HNSCC cells and specimens. Nanog expression was increased in HNSCC cell lines as compared to a normal oral epithelial cell line. Nanog upregulation in clinical tissues from HNSCC patients with recurrent and metastatic specimens relative to the mRNA levels in the samples from normal or primary tissues were examined. Targeting Nanog in HNSCC-SC significantly inhibited their tumorigenic and CSCs-like abilities and effectively increased the sensitivity of HNSCC-SC to chemotherapeutic drug cisplatin treatment. Targeting Nanog in HNSCC-SC showed a synergistic therapeutic effect with cisplatin. Our results suggest that targeting Nanog may have promising therapeutic potential for HNSCC.",
keywords = "Cancer stem cells, Chemosensitivity, Head and neck squamous cell carcinomas, Nanog",
author = "Huang, {Chuan En} and Yu, {Cheng Chia} and Hu, {Fang Wei} and Chou, {Ming Yung} and Tsai, {Lo Lin}",
year = "2014",
month = "8",
day = "25",
doi = "10.3390/ijms150914935",
language = "English",
volume = "15",
pages = "14935--14948",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "MDPI AG",
number = "9",

}

TY - JOUR

T1 - Enhanced Chemosensitivity by Targeting Nanog in Head and Neck Squamous Cell Carcinomas

AU - Huang, Chuan En

AU - Yu, Cheng Chia

AU - Hu, Fang Wei

AU - Chou, Ming Yung

AU - Tsai, Lo Lin

PY - 2014/8/25

Y1 - 2014/8/25

N2 - Chemo-resistance is the major cause of high mortality in head and neck squamous cell carcinomas (HNSCC) in which HNSCC-derived cancer stem cells (CSCs) may be involved. Previously, we enriched a subpopulation of HNSCC-derived spheroid cells (SC) (HNSCC-SC) and identified Nanog as a CSCs marker. The aim of this study was to determine the role of Nanog in the chemosensitivity of HNSCC. The functional and clinicopathological studies of Nanog were investigated in HNSCC cells and specimens. Nanog expression was increased in HNSCC cell lines as compared to a normal oral epithelial cell line. Nanog upregulation in clinical tissues from HNSCC patients with recurrent and metastatic specimens relative to the mRNA levels in the samples from normal or primary tissues were examined. Targeting Nanog in HNSCC-SC significantly inhibited their tumorigenic and CSCs-like abilities and effectively increased the sensitivity of HNSCC-SC to chemotherapeutic drug cisplatin treatment. Targeting Nanog in HNSCC-SC showed a synergistic therapeutic effect with cisplatin. Our results suggest that targeting Nanog may have promising therapeutic potential for HNSCC.

AB - Chemo-resistance is the major cause of high mortality in head and neck squamous cell carcinomas (HNSCC) in which HNSCC-derived cancer stem cells (CSCs) may be involved. Previously, we enriched a subpopulation of HNSCC-derived spheroid cells (SC) (HNSCC-SC) and identified Nanog as a CSCs marker. The aim of this study was to determine the role of Nanog in the chemosensitivity of HNSCC. The functional and clinicopathological studies of Nanog were investigated in HNSCC cells and specimens. Nanog expression was increased in HNSCC cell lines as compared to a normal oral epithelial cell line. Nanog upregulation in clinical tissues from HNSCC patients with recurrent and metastatic specimens relative to the mRNA levels in the samples from normal or primary tissues were examined. Targeting Nanog in HNSCC-SC significantly inhibited their tumorigenic and CSCs-like abilities and effectively increased the sensitivity of HNSCC-SC to chemotherapeutic drug cisplatin treatment. Targeting Nanog in HNSCC-SC showed a synergistic therapeutic effect with cisplatin. Our results suggest that targeting Nanog may have promising therapeutic potential for HNSCC.

KW - Cancer stem cells

KW - Chemosensitivity

KW - Head and neck squamous cell carcinomas

KW - Nanog

UR - http://www.scopus.com/inward/record.url?scp=84906827572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906827572&partnerID=8YFLogxK

U2 - 10.3390/ijms150914935

DO - 10.3390/ijms150914935

M3 - Article

C2 - 25158233

AN - SCOPUS:84906827572

VL - 15

SP - 14935

EP - 14948

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 9

ER -