Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels

Zhengda Sun, Devon A. Lawson, Elizabeth Sinclair, Chih Yang Wang, Ming Derg Lai, Steven W. Hetts, Randall T. Higashida, Christopher F. Dowd, Van V. Halbach, Zena Werb, Hua Su, Daniel L. Cooke

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose To develop a strategy of achieving targeted collection of endothelial cells (ECs) by endovascular methods and analyzing the gene expression profiles of collected single ECs. Methods and results 134 ECs and 37 leukocytes were collected from four patients' intra-iliac artery endovascular guide wires by fluorescence activated cell sorting (FACS) and analyzed by single-cell quantitative RT-PCR for expression profile of 48 genes. Compared to CD45+ leukocytes, the ECs expressed higher levels (p < 0.05) of EC surface markers used on FACS and other EC related genes. The gene expression profile showed that these isolated ECs fell into two clusters, A and B, that differentially expressed 19 genes related to angiogenesis, inflammation and extracellular matrix remodeling, with cluster B ECs have demonstrating similarities to senescent or aging ECs. Conclusion Combination of endovascular device sampling, FACS and single-cell quantitative RT-PCR is a feasible method for analyzing EC gene expression profile in vascular lesions.

Original languageEnglish
Article number106
Pages (from-to)157-165
Number of pages9
JournalBiotechnology Reports
Volume7
DOIs
Publication statusPublished - Sep 1 2015
Externally publishedYes

Keywords

  • Gene expression of artery endothelial cells
  • Single cell quantitative RT-PCR
  • Targeted endothelial cell sampling

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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    Sun, Z., Lawson, D. A., Sinclair, E., Wang, C. Y., Lai, M. D., Hetts, S. W., Higashida, R. T., Dowd, C. F., Halbach, V. V., Werb, Z., Su, H., & Cooke, D. L. (2015). Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels. Biotechnology Reports, 7, 157-165. [106]. https://doi.org/10.1016/j.btre.2015.07.001