Endothelial cell surface expression of protein disulfide isomerase activates β1 and β3 integrins and facilitates dengue virus infection

Shu Wen Wan, Chiou Feng Lin, Yi Tien Lu, Huan Yao Lei, Robert Anderson, Yee Shin Lin

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Infection with dengue virus (DENV) causes diseases ranging from mild dengue fever to severe hemorrhage or shock syndrome. DENV infection of endothelial cells may cause cell apoptosis or vascular leakage and result in clinical illness of hemorrhage. However, the endothelial cell molecules involved in DENV infection and the mechanisms governing virus-cell interactions are still uncertain. Since protein disulfide isomerase (PDI) reducing function at the cell surface was shown to be required for entry of certain viruses and bacteria, we explored the role of PDI expressed on endothelial cell surface in DENV infection. Using siRNA to knock down PDI, DENV infection was reduced which could be reversed by treating cells with a reducing agent Tris(2-carboxyethyl) phosphine hydrochloride (TCEP). DENV-induced PDI surface expression was mediated through the Lys-Asp-Glu-Leu (KDEL) receptor-Src family kinase signal pathway. Furthermore, cell surface PDI colocalized with β1 and β3 integrins after DENV infection, and the activation of integrins was blocked by PDI inhibition. Finally, blockade of PDI inhibited DENV entry into endothelial cells. Our findings suggest a novel mechanism whereby surface PDI which causes integrin activation is involved in DENV entry, and DENV infection further increases PDI surface expression at later time points. These findings may have implications for anti-DENV drug design.

Original languageEnglish
Pages (from-to)1681-1691
Number of pages11
JournalJournal of Cellular Biochemistry
Volume113
Issue number5
DOIs
Publication statusPublished - May 2012
Externally publishedYes

Fingerprint

Protein Disulfide-Isomerases
Dengue Virus
Endothelial cells
Virus Diseases
Viruses
Integrins
Membrane Proteins
Endothelial Cells
Virus Internalization
lysyl-aspartyl-glutamyl-leucine
Hemorrhage
Virus Activation
Chemical activation
src-Family Kinases
Dengue
Drug Design
Reducing Agents
Cell Communication
Small Interfering RNA
Blood Vessels

Keywords

  • INTEGRINS
  • PROTEIN DISULFIDE ISOMERASE
  • VIRAL INFECTION

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Endothelial cell surface expression of protein disulfide isomerase activates β1 and β3 integrins and facilitates dengue virus infection. / Wan, Shu Wen; Lin, Chiou Feng; Lu, Yi Tien; Lei, Huan Yao; Anderson, Robert; Lin, Yee Shin.

In: Journal of Cellular Biochemistry, Vol. 113, No. 5, 05.2012, p. 1681-1691.

Research output: Contribution to journalArticle

Wan, Shu Wen ; Lin, Chiou Feng ; Lu, Yi Tien ; Lei, Huan Yao ; Anderson, Robert ; Lin, Yee Shin. / Endothelial cell surface expression of protein disulfide isomerase activates β1 and β3 integrins and facilitates dengue virus infection. In: Journal of Cellular Biochemistry. 2012 ; Vol. 113, No. 5. pp. 1681-1691.
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