Endothelial Cell Proliferation Assays

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Angiogenesis is the generation of new blood vessels which occurs when a vascular bed is extended, such as in tissue growth, wound healing and tumour formation. In experimental studies, a useful, indirect estimation of angiogenesis activity is obtained by assessing the intensity of proliferation of the endothelial cell component of vascular tissue. Such endothelial cells are often isolated and maintained using common cell culture procedures, so that the effects of various mitogens (growth promoters) on cellular proliferation may be observed in vitro. A number of techniques for assessing cell proliferation are now in common use. In this chapter, we describe the basic methods and relevant principles. The most frequently used in vitro model for studying endothelial cell proliferation employs cultured human umbilical vein endothelial cells (HUVEC), which can be easily obtained. Each of the methods entails some advantages and some intrinsic weaknesses. To achieve the most reliable results, the combined use of two or more methods is to be recommended.

Original languageEnglish
Title of host publicationAngiogenesis Assays: A Critical Appraisal of Current Techniques
PublisherJohn Wiley & Sons, Ltd
Pages39-50
Number of pages12
ISBN (Print)0470016000, 9780470016008
DOIs
Publication statusPublished - Sep 27 2007

Fingerprint

Endothelial cells
Cell proliferation
Assays
Endothelial Cells
Cell Proliferation
Blood Vessels
Tissue
Human Umbilical Vein Endothelial Cells
Blood vessels
Cellular Structures
Growth
Mitogens
Cell culture
Wound Healing
Tumors
Cell Culture Techniques
Neoplasms
In Vitro Techniques

Keywords

  • Cell counting - measuring cell number changes
  • Cell proliferation assays
  • Coulter counter
  • Endothelial cell proliferation
  • Flow cytometry
  • Human umbilical vein endothelial cells (HUVEC)
  • Proliferative activity assessment
  • Vascular tissue endothelial cell component

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lee, W. S. (2007). Endothelial Cell Proliferation Assays. In Angiogenesis Assays: A Critical Appraisal of Current Techniques (pp. 39-50). John Wiley & Sons, Ltd. https://doi.org/10.1002/9780470029350.ch2

Endothelial Cell Proliferation Assays. / Lee, Wen Sen.

Angiogenesis Assays: A Critical Appraisal of Current Techniques. John Wiley & Sons, Ltd, 2007. p. 39-50.

Research output: Chapter in Book/Report/Conference proceedingChapter

Lee, WS 2007, Endothelial Cell Proliferation Assays. in Angiogenesis Assays: A Critical Appraisal of Current Techniques. John Wiley & Sons, Ltd, pp. 39-50. https://doi.org/10.1002/9780470029350.ch2
Lee WS. Endothelial Cell Proliferation Assays. In Angiogenesis Assays: A Critical Appraisal of Current Techniques. John Wiley & Sons, Ltd. 2007. p. 39-50 https://doi.org/10.1002/9780470029350.ch2
Lee, Wen Sen. / Endothelial Cell Proliferation Assays. Angiogenesis Assays: A Critical Appraisal of Current Techniques. John Wiley & Sons, Ltd, 2007. pp. 39-50
@inbook{1cc13a9d3a764317baca28a2b9940f1f,
title = "Endothelial Cell Proliferation Assays",
abstract = "Angiogenesis is the generation of new blood vessels which occurs when a vascular bed is extended, such as in tissue growth, wound healing and tumour formation. In experimental studies, a useful, indirect estimation of angiogenesis activity is obtained by assessing the intensity of proliferation of the endothelial cell component of vascular tissue. Such endothelial cells are often isolated and maintained using common cell culture procedures, so that the effects of various mitogens (growth promoters) on cellular proliferation may be observed in vitro. A number of techniques for assessing cell proliferation are now in common use. In this chapter, we describe the basic methods and relevant principles. The most frequently used in vitro model for studying endothelial cell proliferation employs cultured human umbilical vein endothelial cells (HUVEC), which can be easily obtained. Each of the methods entails some advantages and some intrinsic weaknesses. To achieve the most reliable results, the combined use of two or more methods is to be recommended.",
keywords = "Cell counting - measuring cell number changes, Cell proliferation assays, Coulter counter, Endothelial cell proliferation, Flow cytometry, Human umbilical vein endothelial cells (HUVEC), Proliferative activity assessment, Vascular tissue endothelial cell component",
author = "Lee, {Wen Sen}",
year = "2007",
month = "9",
day = "27",
doi = "10.1002/9780470029350.ch2",
language = "English",
isbn = "0470016000",
pages = "39--50",
booktitle = "Angiogenesis Assays: A Critical Appraisal of Current Techniques",
publisher = "John Wiley & Sons, Ltd",

}

TY - CHAP

T1 - Endothelial Cell Proliferation Assays

AU - Lee, Wen Sen

PY - 2007/9/27

Y1 - 2007/9/27

N2 - Angiogenesis is the generation of new blood vessels which occurs when a vascular bed is extended, such as in tissue growth, wound healing and tumour formation. In experimental studies, a useful, indirect estimation of angiogenesis activity is obtained by assessing the intensity of proliferation of the endothelial cell component of vascular tissue. Such endothelial cells are often isolated and maintained using common cell culture procedures, so that the effects of various mitogens (growth promoters) on cellular proliferation may be observed in vitro. A number of techniques for assessing cell proliferation are now in common use. In this chapter, we describe the basic methods and relevant principles. The most frequently used in vitro model for studying endothelial cell proliferation employs cultured human umbilical vein endothelial cells (HUVEC), which can be easily obtained. Each of the methods entails some advantages and some intrinsic weaknesses. To achieve the most reliable results, the combined use of two or more methods is to be recommended.

AB - Angiogenesis is the generation of new blood vessels which occurs when a vascular bed is extended, such as in tissue growth, wound healing and tumour formation. In experimental studies, a useful, indirect estimation of angiogenesis activity is obtained by assessing the intensity of proliferation of the endothelial cell component of vascular tissue. Such endothelial cells are often isolated and maintained using common cell culture procedures, so that the effects of various mitogens (growth promoters) on cellular proliferation may be observed in vitro. A number of techniques for assessing cell proliferation are now in common use. In this chapter, we describe the basic methods and relevant principles. The most frequently used in vitro model for studying endothelial cell proliferation employs cultured human umbilical vein endothelial cells (HUVEC), which can be easily obtained. Each of the methods entails some advantages and some intrinsic weaknesses. To achieve the most reliable results, the combined use of two or more methods is to be recommended.

KW - Cell counting - measuring cell number changes

KW - Cell proliferation assays

KW - Coulter counter

KW - Endothelial cell proliferation

KW - Flow cytometry

KW - Human umbilical vein endothelial cells (HUVEC)

KW - Proliferative activity assessment

KW - Vascular tissue endothelial cell component

UR - http://www.scopus.com/inward/record.url?scp=84889323156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84889323156&partnerID=8YFLogxK

U2 - 10.1002/9780470029350.ch2

DO - 10.1002/9780470029350.ch2

M3 - Chapter

SN - 0470016000

SN - 9780470016008

SP - 39

EP - 50

BT - Angiogenesis Assays: A Critical Appraisal of Current Techniques

PB - John Wiley & Sons, Ltd

ER -