Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions

Ya Chen Ko, Hsiung Fei Chien, Ya Fen Jiang-Shieh, Chiu Yun Chang, Man Hui Pai, Jian Pei Huang, Hui Min Chen, Ching Hsiang Wu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

CD200 is a highly glycosylated cell surface protein containing two immunoglobulin superfamily domains in the extracellular region and performs immunosuppressive activities. It is widely distributed in various tissues including the vascular endothelium. We report here the distribution of CD200 in rat endothelia from different vascular beds. Endothelial CD200 immunoreactivity was weakly expressed in most arteries but was intensely expressed in the arterioles, most veins and venules, as well as continuous and fenestrated capillaries. The distribution of CD200 in the sinusoidal and lymphatic endothelia was variable. Immunoelectron microscopic studies revealed that endothelial CD200 varied considerably not only in different microvasculatures but also in the membrane domains at the subcellular level. Endothelial CD200 expression was differentially regulated by lipopolysaccharide in cell types both in vivo and in vitro. Functional assessments of endothelial CD200 suggested that the physical binding between CD200 and CD200 receptor (CD200R) was involved in T-cell adhesion to the endothelium but not in macrophage-endothelium interaction. In the latter, however, CD200 agonist, a synthetic peptide from complementarity-determining region 3 of mouse CD200, may trigger CD200R signaling in macrophages to suppress their adhesion to the endothelium. Our findings demonstrate that the distribution, subcellular localization, and lipopolysaccharide-regulation of endothelial CD200 are heterogeneous, and provide evidence elucidating the functional roles of endothelial CD200 during tissue inflammation.

Original languageEnglish
Pages (from-to)183-195
Number of pages13
JournalJournal of Anatomy
Volume214
Issue number1
DOIs
Publication statusPublished - 2009

Fingerprint

endothelium
Cell Communication
Endothelium
Vascular Endothelium
adhesion
Lipopolysaccharides
Lymphatic Endothelium
Macrophages
Complementarity Determining Regions
functional role
Venules
complementarity
Arterioles
cells
Immunosuppressive Agents
Microvessels
Cell Adhesion
peptide
lipopolysaccharides
Veins

Keywords

  • CD200
  • Endothelial cells
  • Heterogeneity
  • Immune cell adhesion
  • Inflammation
  • Lipopolysaccharide
  • Subcellular localization

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Developmental Biology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Cell Biology

Cite this

Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions. / Ko, Ya Chen; Chien, Hsiung Fei; Jiang-Shieh, Ya Fen; Chang, Chiu Yun; Pai, Man Hui; Huang, Jian Pei; Chen, Hui Min; Wu, Ching Hsiang.

In: Journal of Anatomy, Vol. 214, No. 1, 2009, p. 183-195.

Research output: Contribution to journalArticle

Ko, Ya Chen ; Chien, Hsiung Fei ; Jiang-Shieh, Ya Fen ; Chang, Chiu Yun ; Pai, Man Hui ; Huang, Jian Pei ; Chen, Hui Min ; Wu, Ching Hsiang. / Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions. In: Journal of Anatomy. 2009 ; Vol. 214, No. 1. pp. 183-195.
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