Endoplasmic reticulum stress response promotes cytotoxic phenotype of CD8αβ + intraepithelial lymphocytes in a mouse model for Crohn's disease-like ileitis

Jung Su Chang, Soeren Ocvirk, Emanuel Berger, Sigrid Kisling, Uli Binder, Arne Skerra, Amy S. Lee, Dirk Haller

Research output: Contribution to journalArticle

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Abstract

Endoplasmic reticulum (ER) unfolded protein responses (UPR) are implicated in the pathogenesis of inflammatory bowel disease. Cytotoxic CD8αβ + intraepithelial lymphocytes (IEL) contribute to the development of Crohn's disease-like ileitis in TNF ΔARE/+mice. In this study, we characterized the role of ER-UPR mechanisms in contributing to the disease-associated phenotype of cytotoxic IEL under conditions of chronic inflammation. Inflamed TNF ΔARE/+ mice exhibited increased expression of Grp78, ATF6, ATF4, and spliced XBP1 in CD8αβ + IEL but not in CD8αα + IEL or in lamina propria lymphocytes. Chromatin immunoprecipitation analysis in CD8αβ + T cells showed selective recruitment of ER-UPR transducers to the granzyme B gene promoter. Heterozygous Grp78 -/+ mice exhibited an attenuated granzyme B-dependent cytotoxicity of CD8αβ + T cells against intestinal epithelial cells, suggesting a critical activity of this ER-associated chaperone in maintaining a cytotoxic T cell phenotype. Granzyme B-deficient CD8αβ + T cells showed a defect in IL-2-mediated proliferation in Grp78 -/+ mice. Adoptively transferred Grp78 -/+ CD8αβ + T cells had a decreased frequency of accumulation in the intestine of RAG2 -/- recipient mice. The tissue pathology in TNF ΔARE/+ x Grp78 -/+ mice was similar to TNF ΔARE/+ mice, even though the cytotoxic effector functions of CD8αβ +T cells were significantly reduced. In conclusion, ER stress-associated UPR mechanisms promote the development and maintenance of the pathogenic cytotoxic CD8αβ + IEL phenotype in the mouse model of Crohn's disease-like ileitis.

Original languageEnglish
Pages (from-to)1510-1520
Number of pages11
JournalJournal of Immunology
Volume189
Issue number3
DOIs
Publication statusPublished - Aug 1 2012

Fingerprint

Ileitis
Endoplasmic Reticulum Stress
Crohn Disease
Lymphocytes
Phenotype
Unfolded Protein Response
T-Lymphocytes
Granzymes
Endoplasmic Reticulum
Chromatin Immunoprecipitation
Transducers
Inflammatory Bowel Diseases
Intestines
Interleukin-2
Mucous Membrane
Epithelial Cells
Maintenance
Pathology
Inflammation

ASJC Scopus subject areas

  • Immunology

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Endoplasmic reticulum stress response promotes cytotoxic phenotype of CD8αβ + intraepithelial lymphocytes in a mouse model for Crohn's disease-like ileitis. / Chang, Jung Su; Ocvirk, Soeren; Berger, Emanuel; Kisling, Sigrid; Binder, Uli; Skerra, Arne; Lee, Amy S.; Haller, Dirk.

In: Journal of Immunology, Vol. 189, No. 3, 01.08.2012, p. 1510-1520.

Research output: Contribution to journalArticle

Chang, Jung Su ; Ocvirk, Soeren ; Berger, Emanuel ; Kisling, Sigrid ; Binder, Uli ; Skerra, Arne ; Lee, Amy S. ; Haller, Dirk. / Endoplasmic reticulum stress response promotes cytotoxic phenotype of CD8αβ + intraepithelial lymphocytes in a mouse model for Crohn's disease-like ileitis. In: Journal of Immunology. 2012 ; Vol. 189, No. 3. pp. 1510-1520.
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