Endoplasmic reticulum ribosome-binding protein 1 (RRBP1) overexpression is frequently found in lung cancer patients and alleviates intracellular stress-induced apoptosis through the enhancement of GRP78

H. Y. Tsai, Y. F. Yang, A. T. Wu, C. J. Yang, Y. P. Liu, Y. H. Jan, C. H. Lee, Y. W. Hsiao, Chi-Tai Yeh, C. N. Shen, P. J. Lu, M. S. Huang, M. Hsiao

Research output: Contribution to journalArticle

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Abstract

Lung cancer is the leading cause of cancer deaths and is the most occurring malignancy worldwide. Unraveling the molecular mechanisms involved in lung tumorigenesis will greatly improve therapy. During early tumorigenesis, rapid proliferating tumor cells require increased activity of endoplasmic reticulum (ER) for protein synthesis, folding and secretion, thereby are subjected to ER stress. Ribosome-binding protein 1 (RRBP1) was originally identified as a ribosome-binding protein located on the rough ER and associated with unfolding protein response (UPR). In this report, we investigated the role of RRBP1 in lung cancer. RRBP1 was highly expressed in lung cancer tissue, as compared with adjacent normal tissues as assessed by immunohistochemistry (IHC) using lung cancer tissue array (n=87). Knockdown of RRBP1 by short-hairpin RNAs caused ER stress and significantly reduced cell viability and tumorigenicity. This effect was associated with a significant reduction in the expression of glucose-regulated protein 78 (GRP78). UPR regulator GRP78, an anti-apoptotic protein that is widely upregulated in cancer, has a critical role in chemotherapy resistance in some cancers. According to our results, cells with a higher level of RRBP1 were more resistant to ER stress. Ectopic expression of RRBP1 alleviated apoptosis that was induced by the ER-stress agent tunicamycin, 2-deoxy-D-glucose (2DG) or doxorubicin via enhancing GRP78 protein expression. A strong correlation was observed between the expression of RRBP1 and GRP78 in tumor biopsies using the database GSE10072. Our results also indicated that RRBP1 may involve in the regulation of mRNA stability of UPR components including ATF6 and GRP78. Taken together, RRBP1 could alleviate ER stress and help cancer cell survive. RRBP1 is critical for tumor cell survival, which may make it a useful target in lung cancer treatment and a candidate for the development of new targeted therapeutics.

Original languageEnglish
Pages (from-to)4921-4931
Number of pages11
JournalOncogene
Volume32
Issue number41
DOIs
Publication statusPublished - Oct 10 2013

Fingerprint

Reticulum
Ribosomes
Endoplasmic Reticulum
Lung Neoplasms
Carrier Proteins
Apoptosis
Endoplasmic Reticulum Stress
Unfolded Protein Response
Neoplasms
glucose-regulated proteins
Cell Survival
Carcinogenesis
Tunicamycin
Apoptosis Regulatory Proteins
Rough Endoplasmic Reticulum
Protein Folding
RNA Stability
Deoxyglucose
Doxorubicin
Small Interfering RNA

Keywords

  • apoptosis
  • GRP78
  • lung cancer
  • RRBP1
  • UPR

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Endoplasmic reticulum ribosome-binding protein 1 (RRBP1) overexpression is frequently found in lung cancer patients and alleviates intracellular stress-induced apoptosis through the enhancement of GRP78. / Tsai, H. Y.; Yang, Y. F.; Wu, A. T.; Yang, C. J.; Liu, Y. P.; Jan, Y. H.; Lee, C. H.; Hsiao, Y. W.; Yeh, Chi-Tai; Shen, C. N.; Lu, P. J.; Huang, M. S.; Hsiao, M.

In: Oncogene, Vol. 32, No. 41, 10.10.2013, p. 4921-4931.

Research output: Contribution to journalArticle

Tsai, H. Y. ; Yang, Y. F. ; Wu, A. T. ; Yang, C. J. ; Liu, Y. P. ; Jan, Y. H. ; Lee, C. H. ; Hsiao, Y. W. ; Yeh, Chi-Tai ; Shen, C. N. ; Lu, P. J. ; Huang, M. S. ; Hsiao, M. / Endoplasmic reticulum ribosome-binding protein 1 (RRBP1) overexpression is frequently found in lung cancer patients and alleviates intracellular stress-induced apoptosis through the enhancement of GRP78. In: Oncogene. 2013 ; Vol. 32, No. 41. pp. 4921-4931.
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